Molecular Mechanisms of TachyplesinⅠRole on Genomic DNA and RNA of Escherichia coli

doi:10.3969/j.issn.10080864.2013.01.12

›› 2013, Vol. 15 ›› Issue (1): 71-75.DOI: 10.3969/j.issn.10080864.2013.01.12

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Molecular Mechanisms of TachyplesinⅠRole on Genomic DNA and RNA of Escherichia coli

HONG Jun, HU Jianye, WANG Fumei, CHEN Lanying*, WANG Zhe

(Department of Bioengineering, Henan University of Urban Construction, Henan Pingdingshan 467036, China)

Online:2013-02-15 Published:2013-02-26

鲎源抗菌肽对大肠杆菌基因组DNA和RNA作用的分子机制

洪军,胡建业,王福梅,陈兰英*,王者

(河南城建学院生物工程系, 河南平顶山 467036)

通讯作者: 陈兰英,教授,主要从事分子生物学研究。 Email:lychen666@163.com
作者简介:洪军,讲师,博士,主要从事多肽作用机理研究。Email:hongjun@hncj.edu.cn。
基金资助:
河南城建学院博士启动基金（2012JBS002)资助。

Abstract

Abstract:

In order to investigate the molecular mechanism of tachyplesinⅠrole on bacterial genome, the genomic DNA and RNA binding ability of tachyplesinⅠwas examined by agarose gel retardation electrophoresis, fluorescence and UV spectroscopy in this experiment. The results indicated that tachyplesinⅠhad effect on genomic DNA in a concentrationdependent manner, and the high concentrations of tachyplesinⅠ(above or equal to 80 μg/mL) could lead to genomic DNA breaking. TachyplesinⅠcould combine with genomic DNA and RNA, and inhibit their migration. The mode of combining tachyplesinⅠand genomic DNA needs further study. The results provided a theoretical basis for further understanding the sterilization mechanism of tachyplesinⅠat molecular level.

Key words: tachyplesinⅠ, Escherichia coli, genomic DNA, RNA, interaction

摘要：

为了探讨鲎素对细菌基因组DNA和RNA作用的分子机制，采用琼脂糖凝胶阻滞电泳、荧光和紫外光谱的方法研究了鲎素对细菌基因组DNA和RNA的影响，结果表明鲎素能够与大肠杆菌基因组DNA发生作用，并呈浓度依赖关系，浓度越高对细菌基因组DNA损伤越大；鲎素也能与基因组DNA和RNA结合，抑制其迁移。鲎素与基因组DNA的结合方式有待进一步研究，此结果为在分子水平上深入了解鲎素的杀菌机制提供重要的理论依据。

关键词: 鲎素;大肠杆菌;基因组DNA;RNA;相互作用

CLC Number:

Q819

HONG Jun, HU Jianye, WANG Fumei, CHEN Lanying*, WANG Zhe. Molecular Mechanisms of TachyplesinⅠRole on Genomic DNA and RNA of Escherichia coli[J]. , 2013, 15(1): 71-75.

洪军,胡建业,王福梅,陈兰英*,王者. 鲎源抗菌肽对大肠杆菌基因组DNA和RNA作用的分子机制[J]. , 2013, 15(1): 71-75.

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Molecular Mechanisms of TachyplesinⅠRole on Genomic DNA and RNA of Escherichia coli

鲎源抗菌肽对大肠杆菌基因组DNA和RNA作用的分子机制

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