Research of High Resistance to Turnip Mosaic Virus Mediated by Coat Protein Gene Segment

doi:doi:10.3969/j.issn.1008-0864.2013.02.13

›› 2013, Vol. 15 ›› Issue (2): 83-88.DOI: doi:10.3969/j.issn.1008-0864.2013.02.13

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Research of High Resistance to Turnip Mosaic Virus Mediated by Coat Protein Gene Segment

ZENG Gang1,2, YE Yan-ying1, CAO Ming-qing1, MA Rong-cai1, TANG Le-chen2, YAO Lei1*

(1.Beijing Ago-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097|2.Fujian Agriculture and Forestry University, Fuzhou 350002, China)

Received:2012-09-13 Revised:2012-10-25 Online:2013-04-15 Published:2013-04-16

外壳蛋白基因片段介导的高抗TuMV研究

曾钢1,2,叶艳英1,曹鸣庆1,马荣才1,唐乐尘2,姚磊1*

(1.北京市农林科学院农业生物技术研究中心, 北京 100097|2.福建农林大学, 福州 350002)

通讯作者: 姚磊,副研究员,博士,研究方向为植物生物学与生物技术。E-mail:yaolei@baafs.net.cn
作者简介:曾钢,硕士研究生,研究方向为植物病理学与植物生理学。E-mail:unique133@126.com。
基金资助:
国家863计划项目(2008AA10Z154);北京市自然科学基金(6092010)资助。

Abstract

Abstract:

In order to get stable high resistance to turnip mosaic virus (TuMV), a 377-nucleotide (nt) segment near 3′ conserved region of TuMV coat protein (CP) gene was chosen to construct hairpin RNAi vector, and transformed into Arabidopsis thaliana. The transgenic plants were inoculated with TuMV isolate BJ-C4, which was of high pathogenicity and mainly epidemic in Beijing area. Three of the 8 transgenic lines showed hyper-resistance to TuMV. Quantitative PCR results revealed that the accumulation of virus was hardly to detect in highly resistant transgenic plants.

Key words: turnip mosaic virus, coat protein gene, hairpin structure, RNAi, resistance

摘要：

为了获得对芜菁花叶病毒（TuMV）高度稳定的抗性，选取TuMV的外壳蛋白（CP）基因近3′端保守区共377 bp的核苷酸序列，构建了抗TuMV的发卡结构RNAi载体，并转化了TuMV的天然宿主拟南芥。转基因植株用北京地区TuMV主要流行的强致病株系BJ-C4人工接种。鉴定的8个转基因株系中有3个株系表现出对TuMV的高度抗性。定量PCR结果显示，高抗转基因株系体内几乎检测不到病毒的积累。

关键词: 芜菁花叶病毒;外壳蛋白基因;发卡结构;RNA干扰;抗性

CLC Number:

ZENG Gang1,2, YE Yan-ying1, CAO Ming-qing1, MA Rong-cai1, TANG Le-chen2, YAO Lei. Research of High Resistance to Turnip Mosaic Virus Mediated by Coat Protein Gene Segment[J]. , 2013, 15(2): 83-88.

曾钢1,2,叶艳英1,曹鸣庆1,马荣才1,唐乐尘2,姚磊1*. 外壳蛋白基因片段介导的高抗TuMV研究[J]. , 2013, 15(2): 83-88.

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Research of High Resistance to Turnip Mosaic Virus Mediated by Coat Protein Gene Segment

外壳蛋白基因片段介导的高抗TuMV研究

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