Abstract: In order to optimize the liquid fermentation culture of Agaricus blazei Murrill, based on single-factor experiment, taking usage of SMS extract of Pleurotus eryngii (referred to SMS) (A), yeast extract (B) and corn starch (C) as effect factors and mycelial dry weight (Y) as response value, this paper studied the parameters of liquid fermentation culture of Agaricus blazei Murrill and their interaction by 3 factors and 3 levels Box-Behnken designation combined with respond surface methodology. The results showed that parameters A, B, C and the interaction of AC on mycelial dry weight was extremely significant. The optimum composition for liquid fermentation culture were SMS (153.54 g/L), yeast extract (9.74 g/L) and corn starch (30.51 g/L). The maximum biomass of mycelial reached 1.785 g/100 mL, which was very close to the model predictive value ( 1.796 g/100 mL), and the relative error value was only 0.62%.

Key words: Agaricus blazei Murrill, SMS extract of Pleurotus eryngii, liquid medium, mycelial, biomass, response surface methodology

摘要： 为了优化巴西蘑菇（Agaricus blazei Murrill）的液体发酵培养，在单因素试验基础上，以杏鲍菇（Pleurotus Eryngii）菌渣提取液（A）、酵母浸膏（B）和玉米粉（C）用量作为影响因子，巴西蘑菇的菌丝体干重（Y）为响应值，采用3因素3水平的Box-Behnken试验设计及响应面分析法对巴西蘑菇液体培养基各参数及其交互作用进行了研究。结果表明：因素A、B、C及AC的交互作用对巴西蘑菇菌丝体干重影响均达到了极显著水平，液体发酵培养基最优组分为：杏鲍菇菌渣153.5 g/L，酵母浸膏9.7 g/L，玉米粉30.5 g/L，其菌丝体生物量最大为1.785 g/100 mL，与试验模型预测值（1.796 g/100 mL）的相对误差值仅为0.62%。

关键词: 巴西蘑菇, 杏鲍菇菌渣, 液体培养基, 菌丝体, 生物量, 响应面分析法