Journal of Agricultural Science and Technology ›› 2020, Vol. 22 ›› Issue (3): 173-180.DOI: 10.13304/j.nykjdb.2018.0778
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GUO Huihui, LIN Congfa, JIANG Yuanbin, LI Zhigang
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郭慧慧,林丛发,蒋元斌,李志刚
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Abstract: In order to explore sustainable development of Dendrobium huoshanense resources, this paper used response surface method to optimize the protocorm multiplication medium. On the basis of singlefactor tests, the mathematical regression model was established about the dependent variable (proliferation rate) through BoxBenhnken center composite design and response surface analysis. The results showed that the concentration of NAA and 6benzylaminopurine (6BA) had the most significant effect on the proliferation rate, and the interaction between 6BA and potato was significant. The suitable concentrations of NAA, 6BA and potato were 0.05 mg·L-1, 0.70 mg·L-1 and 185 g·L-1, respectively. At this time, the proliferation rate was 2 989.82% +205.55%, which was consistent with the predicted value of the model. Above results indicated that the response surface methodology was viable for optimizing medium composition in improving proliferation rate.
Key words: Dendrobium huoshanense, Response surface methodology, Protocorm proliferation, Culture medium, Optimum formulation
摘要: 为可持续开发霍山石斛资源,采用响应面法优化霍山石斛原球茎增殖培养基。在单因素试验基础上,根据BoxBehnken试验设计原理,采用三因素三水平的响应面分析法,以增殖率为响应值进行回归分析。结果表明,萘乙酸(简称NAA)及6苄氨基嘌呤(简称6BA)浓度对增殖率影响最为显著,6BA与马铃薯两者间交互作用显著,适宜的NAA、6BA及马铃薯浓度分别为0.05 mg·L-1、0.70 mg·L-1和185 g·L-1,此时增殖率为2 989.82%±205.55%,与模型预测值基本符合。响应面法优化霍山石斛原球茎增殖培养基切实可行。
关键词: 霍山石斛, 响应面试验, 原球茎增殖, 培养基, 优化配方
GUO Huihui, LIN Congfa, JIANG Yuanbin, LI Zhigang. Optimization of Protocorm Proliferation Medium of Dendrobium huoshanense by Response Surface Methodology[J]. Journal of Agricultural Science and Technology, 2020, 22(3): 173-180.
郭慧慧,林丛发,蒋元斌,李志刚. 响应面试验优化霍山石斛原球茎增殖培养基[J]. 中国农业科技导报, 2020, 22(3): 173-180.
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URL: https://www.nkdb.net/EN/10.13304/j.nykjdb.2018.0778
https://www.nkdb.net/EN/Y2020/V22/I3/173