Abstract: In order to explore the feasibility of microwave-assisted procedure for  extraction polysaccharide from Evodia rutaecarpa, on the basis of single factor experiments with three factors and three levels of response surface analysis, this paper won the second equation of linear regression (P<0.01) using the software Box-Behnken experiment design principle. The response surface figure were built with polysaccharide extraction yield as response values to determine the optimum parameters of microwave extraction of Evodia rutaecarpa polysaccharide as follows: microwave power of 400 W, extraction time of 100 s, extraction times of 2, and solid-liquid ratio of 1∶100; Evodia rutaecarpa polysaccharide extraction rate was 21.7%（predictive value of 21.9%, the traditional water extraction was only 12.3%）, validation experiments showed  that the model equation can well predict the result of the experiment, the fitting was good.

Key words: microwave-assisted extraction, response surface methodology, Evodia rutaecarpa polysaccharide

摘要： 为探索微波辅助提取吴茱萸多糖工艺的可行性，在单因素实验基础上采用三因素三水平响应面分析法，利用软件Box-Behnken 实验设计原理，获得二次线性回归方程式（整体模型P<0.01）。以多糖提取率为响应值作响应面图，确定微波提取吴茱萸多糖的优化工艺条件修正为：微波功率400 W、提取时间为100S、提取次数2次、料液比为1∶100，吴茱萸多糖实际提取率为21.01%（预测值为21.9%，传统水提仅为12.3%），验证实验表明，所得模型方程能较好地预测实验结果，拟合度较好。

关键词: 微波辅助提取, 响应面法, 吴茱萸多糖