Abstract: Synthetic pesticides used to control plant parasitic nematodes have seriously polluted the ecological environment. Therefore it is urgent to develop natural biological pesticides. All nematode species are similar, and the difference between parasitic nematodes and model animal Caenorhabditis elegansis are less than the difference between species of parasitic nematodes. Thus the present study investigated the effect of thymol, a natural product from Thymus mongolicus Ronn., on C. elegans  at the stages of growth and development (L4 stage) and reproductive (egg stage). The lifespan, locomotion capacity and survival sub-algebraic phenotype of C. elegansis were examined. The results showed that the median lethal concentration and minimum lethal concentration was 50 mg/L and 125 mg/L, respectively, when the C. elegans at L4 stage was treated with thymol for 4 h. When C. elegans at egg stage was treated with thymol for 4 h, the median lethal concentration and minimum lethal concentration was 50 mg/L and 100 mg/L, respectively. Meanwhile thymol significantly reduced the athletic ability of C. elegans at L4 and egg stages, and the surviving progeny number of C. elegans at egg stage. Sum up, the treatment with 125 mg/L thymol on nematodes for 4 h had the best insecticidal effect and could control plant parasitic nematodes with low cost,  high efficiency and environmental friendly.

Key words: thymol, Caenorhabditis elegans, biological control

摘要： 多年来，防治植物寄生性线虫的合成农药严重污染生态环境，开发天然生物型农药迫在眉睫。基于线虫动物门所有物种都表现出相似性，且寄生性线虫与模式动物秀丽线虫(Caenorhabditis elegans)之间的差异小于不同种类寄生性线虫之间的差异，以模式生物秀丽线虫为研究对象，探究植物天然产物百里香酚对生长发育期（L4时期）和生殖时期（有卵时期）秀丽线虫的寿命、运动能力、成活子代数目表型指标的影响。结果表明，百里香酚处理L4时期的秀丽线虫4 h，其半数致死浓度为50 mg/L，最低致死浓度为125 mg/L。百里香酚处理有卵时期的秀丽线虫4 h，其半数致死浓度为50 mg/L，最低致死浓度为100 mg/L。同时，百里香酚能显著性降低上述两个时期秀丽线虫的运动能力以及有卵时期秀丽线虫的成活子代数目。综合以上结果，选取125 mg/L的百里香酚处理线虫4 h，其杀虫效果最佳，能达到低成本、高效、环保的防治植物寄生性线虫的目的。

关键词: 百里香酚, 秀丽线虫, 生物防治