Abstract: Acer Palmatum cv. ‘Sango kaku’ is a precious ornamental tree with colorful foliage in growing season and red stems in winter. In order to establish the tissue culture regeneration system, taking the bud stem segment of ‘Sango kaku’  as explant,   the sterilization and medium for induction, proliferation and rooting were selected, and the transplanting techniques were established in vitro plants. The results showed that, June was the best timimg of the whole year for explant collection. The explant samples were sterilized with 75% ethanol for 30 s plus 0.1%  mercury for 10 min after immersion in 400× Carbendazim, which was the best sterilization way. The optimum medium for each stage of the regeneration system was: ① induction culture: MS+0.5 mg·L-1 IAA; ② proliferation culture: MS+2.0 mg·L-1 6-BA+0.5 mg·L-1 IAA; ③ rooting culture: 1/2 MS+0.2 mg·L-1 NAA. The tissue culture seedlings were kept in closed bottle for 6 d, in opening bottle for 3 d, and transplanted to the matrix with charcoal∶vermiculite∶perlite = 1∶1∶1. The survival rate of the transplanted tissue culture plants could reach 90%.

Key words: Acer palmatum, ‘Sango kaku’, tissue culture, regeneration system

摘要： 鸡爪槭品种‘赤枫’是稀有的生长季观叶，冬季观枝的园林彩化树种。为了建立合理的组培再生体系，在短时期内获得大量种苗，以‘赤枫’带芽茎段为外植体，对其灭菌方式及启动、增殖、生根培养基进行了筛选，并对所获组培苗移栽技术进行了探讨。结果显示：全年中6月份为最佳外植体采集时间，外植体经50%多菌灵400倍液浸1 h后，以75％乙醇30 s+0.1％升汞10 min浸泡处理的灭菌效果最佳。再生体系各阶段最适培养基为：①启动培养：MS+0.5 mg·L-1 IAA；②增殖培养：MS+2.0 mg·L-1 6-BA+0.5 mg·L-1 IAA；③生根培养：1/2MS+0.3 mg·L-1 NAA。组培苗闭瓶炼苗6 d，开瓶炼苗3 d后，移栽到草炭∶蛭石∶珍珠岩=1∶1∶1的基质中，炼苗成活率可达90%。

关键词: 鸡爪槭；&lsquo, 赤枫&rsquo, ；组培；再生体系