Abstract:

A plant expression vector pUmG2 harboring the glyphosate-tolerant mG2-epsps gene was constructed and transferred into the maize inbred line X90 by pollen-tube pathway. Among the 1 542 transformed plants of TO generation, 32 glyphosate-tolerant plants were survived after sprayed with 0.25% glyphosate, and 29 of them were con- firmed positive plants by PCR analysis. The average transformation rate was 1.88%. Southern blot and Western blot showed that the rnG2-epsps gene had been introduced into maize genome and expressed correctly.

Key words: pollen-tube pathway, glyphosate, mG2-epsps gene, transgenic maize

摘要：

构建了含有耐草甘膦基因mG2-epsps的植物表达载体pUmG2，采用花粉管通道法将其导入优良玉米自交系X90中，对Tn代1542株植株进行草甘膦喷洒实验，共得到32株能够耐受0．25％草甘膦药液的抗性植株；对这32株抗性植株的PCR检测结果表明，其中29株为PCR阳性植株，平均转化率为1．88％；Southern杂交和Western杂交检测结果证明mG2-epspps基因已整合至玉米基因组并正确表达。

关键词: 花粉管通道法 草甘膦 raG2-epsps基因 转基因玉米