Abstract:

A full-length eDNA encoding the light harvesting chlorophyll a/b binding protein was cloned firstly by RT-PCR and RACE-PCR from Populus euphratica, designated PeCab （GenBank accession number： EU078170 ）. Sequence analysis showed that the length of PeCab is 992 bp, including a complete open reading frame of 792 bp, encoding a protein of 263 amino acids. It belonged to type Ⅰ of PS Ⅱ. PeCab displayed high sequence identity with previously cloned Cab family genes of plant and was clustered close to Cab family genes of dicotyledon plants. Semiquantitative RT-PCR showed that PeCab was greatly induced by light and was responded to 6-BA,GA3 and NAA. The expression of PeCab did not change at transcriptional stage under darkness and ABA solution treatment. The research is not only to enrich the gene resource of Cab gene family, but also to establish the foundation for discovering the mechanism of light and various conditional adaption of Populus euphratica.

Key words: Populus euphratica , light harvesting chlorophyll a/b binding protein , sequence analysis , semi-quantitative RT-PCR

摘要：

采用RT-PCR和RACE—PCR技术，首次从胡杨中克隆到捕光叶绿素a／b结合蛋白基因全长cDNA，命名为PeCab（GenBank序列号：EU078170）。序列分析表明：该基因全长为992bp，开放阅读框为792bp，编码263个氨基酸，属于光系统Ⅱ类型ⅠCab基因，与植物Cab家族基因具有较高同源性，并且与双子叶植物的Cab家族基因的亲缘关系更近。半定量RT—PCR分析表明，该基因受光诱导表达，对6-BA、GA3和NAA三种激素的诱导均有应答，但在黑暗和ABA激素处理下，PeCab基因在转录水平上的表达基本没有变化。本研究丰富了Cab家族基因库资源，对揭示胡杨光保护及对各种环境适应性机理的研究奠定了基础。

关键词: 胡杨 叶绿素a／b结合蛋白 序列特性分析 半定量RT-PCR