Abstract:

Using saltingout method, combined with Sephacryl S-200HR gel filtration chromatography and DEAE Sepharose F.F. ion exchange chromatography, linoleate isomerase was isolated and purified from P. freudenreichii ssp. shermanii and L. plantarum, respectively. linoleate isomerase from different sources showed different elution characteristics in the purification process. The molecular weight of linoleate isomerase from P. freudenreichii ssp. shermanii was 56 kDa, and that from L. plantarum was 50.65 kDa. The result indicated that big differences existed in the purification method of linoleate isomerase due to their different sources.

Key words: conjugated linoleic acid (CLA);P. freudenreichii ssp. shermanii; , L. plantarum, linoleate isomerase

摘要：

采用盐析、Sephacryl S-200HR凝胶过滤层析、DEAE Sepharose F.F.离子交换层析相结合的方法，分别建立了来源于费氏丙酸杆菌谢氏亚种（P. feudenreichii ssp. shermanii）和植物乳杆菌（L. plantarum）的亚油酸异构酶的分离提取步骤。结果表明不同来源的亚油酸异构酶在纯化的过程中表现出不同的洗脱特性，P. freudenreichii ssp. shermanii亚油酸异构酶的分子质量约为56 kDa, L. plantarum亚油酸异构酶的分子质量为50.65 kDa。不同来源亚油酸异构酶的分离纯化方法存在差异。

关键词: 共轭亚油酸（CLA）；费氏丙酸杆菌费氏亚种（P. freudenreichii ssp. shermanii）；植物乳杆菌（L. plantarum）；亚油酸异构酶