Abstract:

55 strains of lactic acid bacteria were isolated from traditional homemade fermented milk products in 5 different regions of Mongolia. All strains were identified on basis of restriction fragment length polymorphism (RFLP) analysis and 16S rDNA sequences compared with 17 standard strains. RFLP analysis used a set of restriction enzymes, AluⅠ, HaeⅢ, BsmaⅠ, TspRⅠand HinfⅠ. The results showed that 49 Lactobacilli strains and 2 Pdeiococcus strains were identified to species level, while the other 4 cocci strains were identified at genus level. 16S rDNARFLP method was of accuracy, reliability and efficiency for identifying lactic acid bacteria.

Key words: lactic acid bacteria, rapid identification, restriction fragment length polymorphism (RFLP), 16S rDNA sequence analysis

摘要：

以17株标准菌株为参照，采用限制性片段长度多态性（RFLP）分析和16S rDNA序列分析相结合的技术，对分离自蒙古国5个地区传统乳制品中的55株乳酸菌进行了快速鉴定。结果表明,通过限制性内切酶AluⅠ、HaeⅢ、BsmaⅠ、TspRⅠ和HinfⅠ的指纹图谱分析，将49株乳杆菌和2株片球菌准确鉴定到种，其他4株肠球菌鉴定到属。采用16S rDNA-RFLP方法鉴定乳酸菌具有准确性，可靠性和高效性。

关键词: 乳酸菌；快速鉴定；限制性片段长度多态性(RFLP)；16S rDNA序列分析