关键词: 植烟土壤, 连作, 高通量测序, 细菌, 真菌, 多样性

Abstract: In order to study the effects of different fertilization treatments on soil environmental factors and microbial community diversity in continuous cropping tobacco plants, and understand the relationship between soil microbial community and environmental factors, this paper adopted IlluminaMiSeq high-throughput sequencing technique to sequence bacteria 16S rRNAV4-V5 district and fungi ITS rRNAV4-V5 district in soil of continuous cropping tobacco plants under different fertilization treatments and studied the relationship between 8 environmental factors(organic matter, available nitrogen, available phosphorus, available K, protease, catalase, phosphatase, sucrase ) and the microbes by principal component analysis and redundancy analysis. The results showed that after sequencing, 264 500 effective sequences and 22 035 operation classification units (OTUs) were obtained. Colonies showed that the fertilization treatment increased the community composition of bacteria and fungi in the soil compared with that of the contrast. There were bigger differences in bacterial and fungal diversity between fertilization treatment and the CK. The results showed that fertilization could significantly change the structure of bacteria and fungi in soil. The content of available K could affect the diversity and abundance of soil bacterial community in a certain range, and the 8 environmental factors could affect the diversity and abundance of fungi by different degree. The soil microbial information of tobacco rhizosphere obtained by high-throughput sequencing technology was rich, and provided theoretical references for different fertilization treatments affecting soil environmental factors and microbial community diversity.

Key words: tobacco planted soil, continuous cropping, high-throughput sequencing, bacteria, fungi, diversity