中国农业科技导报 ›› 2021, Vol. 23 ›› Issue (3): 91-98.DOI: 10.13304/j.nykjdb.2019.0920

• 食品质量 加工储运 • 上一篇    下一篇

食品中产毒真菌核酸检测方法的研究进展

张庆1,2,赵晓美2,王娉2,刘冰1*,陈颖2*   

  1. 1.天津科技大学食品科学与工程学院, 食品营养与安全国家重点实验室, 省部共建食品营养与安全重点实验室, 天津 300457; 
    2.中国检验检疫科学研究院农产品安全研究中心, 北京 100176
  • 收稿日期:2019-11-05 出版日期:2021-03-15 发布日期:2020-03-10
  • 通讯作者: 陈颖 E-mail: chenyingcaiq@163.com; 刘冰 E-mail: ice6377@tust.edu.cn
  • 作者简介:张庆 E-mail:987666458@qq.com
  • 基金资助:
    国家重点研发计划项目(2017YFC1601205)

Advance on Detection Methods of Nucleic Acid for Toxin-Producing Fungi in Food

ZHANG Qing1,2, ZHAO Xiaomei2, WANG Ping2, LIU Bing1*, CHEN Ying2*   

  1. 1.State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education; College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China;
    2.Agro-Product Safety Research Center, Chinese Academy of Inspection and Quarantine, Beijing 100176, China
  • Received:2019-11-05 Online:2021-03-15 Published:2020-03-10

摘要: 食品中最常见的产毒真菌主要包括黄曲霉、赭曲霉、青霉属和镰刀菌属等,能够对人和动物机体造成不可逆转的伤害甚至死亡。针对产毒真菌检测可在毒素形成早期预测其产毒性能,为前置化干预毒素危害提供技术支撑,概述了近年来应用于食品中产毒真菌检测的常规PCR方法、实时荧光定量PCR方法和DNA指纹图谱方法、DNA条形码方法等,并对未来产毒真菌检测方法的发展趋势进行了展望。

关键词: 产毒真菌, 核酸, 检测方法

Abstract: The most common toxin-producing fungi in foods include Aspergillus flavus, Aspergillus ochraceus, Penicillium spp. and Fusarium spp., which can cause irreversible damage or even death to human and animal organisms. Detection of toxin-producing fungi can predict its toxin-producing performance in the early stages of toxin formation, and provide technical support for pre-treatment intervention of toxin damage. This paper summarized the conventional PCR methods, real-time fluorescent quantitative PCR method, DNA fingerprinting method and DNA barcode method for the detection of toxin-producing fungi in food, and prospected the development trend of toxin-producing fungi detection methods in the future.

Key words: toxin-producing fungi, nucleic acid, detection method