中国农业科技导报 ›› 2018, Vol. 20 ›› Issue (4): 29-35.DOI: 10.13304/j.nykjdb.2017.0391

• 生物技术 生命科学 • 上一篇    下一篇

施用有机肥对成熟期烤烟质体色素的影响

黄明月,牛德新,王立志,娄亚楠,李艳华,杨胜男,张洪映,崔红*   

  1. 河南农业大学烟草学院, 国家烟草栽培生理生化基地, 郑州 450002
  • 收稿日期:2017-06-19 出版日期:2018-04-15 发布日期:2017-08-25
  • 通讯作者: 崔红,教授,博士,研究方向为烟草生物技术。 E-mail:cuihonger_13@163.com
  • 作者简介:黄明月,硕士研究生,研究方向为烟草生物技术。E-mail:huangmy8601@163.com。
  • 基金资助:
    中国烟草总公司特色优质烟叶开发重大专项(110200902045 TS-01)资助。

Effects of Organic Fertilizer Application on the Biosynthesis of Plastid Pigment in Flue-cured Tobacco

HUANG Mingyue, NIU Dexin, WANG Lizhi, LOU Yanan, LI Yanhua,YANG Shengnan, ZHANG Hongying, CUI Hong*   

  1. National Tobacco Cultivation & Physiology & Biochemistry Research Center, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2017-06-19 Online:2018-04-15 Published:2017-08-25

摘要: 为揭示有机肥对烤烟质体色素代谢的影响机理,研究了不同有机肥施用量下,烤烟品种云烟87成熟期中部叶的叶绿体超微结构,质体色素含量和质体色素合成途径关键基因的表达模式。结果表明:随有机肥施用量的增加,叶绿体膜上的嗜锇颗粒和类囊体片层数量增多,淀粉粒的数目和体积增加;有机肥促进烟叶中叶绿素和类胡萝卜素的合成;质体色素合成基因的表达模式分析显示,烟草DXS、DXR、LCYb、LCYe和CRTISO基因是质体色素合成途径的关键基因,受有机肥的诱导表达,而IPI、PSY、PDS、ZDS、CAB和CHL基因不受有机肥的诱导表达。

关键词: 有机肥, 烤烟, 质体色素, 超微结构, 基因表达

Abstract: In order to uncover the effects of organic fertilizers on the metabolism of plastid pigment in flue-cured tobacco, this study compared the plastid ultrastructure, plastid pigment contents and pigment biosynthesis-key gene expression patterns in the middle leaves of flue-cured tobacco cultivar Yunyan 87 at the ripening stage under different organic fertilizer treatments. The results showed that the number of osmiophilic granules and thylakoid lamellas increased along with the increase of organic fertilizer application. Notably, high organic fertilizer application could markedly promoted the chloroplast (chlorophyll a and chlorophyll b) development and plastid pigment (β-carotene, violaxanthin, neoxanthin and lutein) accumulation. Analysis of pigment biosynthesis-key gene expression patterns revealed that the transcript levels of DXS, DXR, LCYb, LCYe and CRTISO genes were responded to organic fertilizers, indicating that these genes may play crucial roles in pigment synthesis, while IPI, PSY, PDS, ZDS, CAB and CHL genes were not induced by organic fertilizer.

Key words: organic fertilizer, flue-cured tobacco, plastid pigment, ultrastructure, gene expression