小麦赤霉病生防菌的筛选及其田间防效研究

doi:10.13304/j.nykjdb.2019.0406

中国农业科技导报 ›› 2020, Vol. 22 ›› Issue (1): 67-77.DOI: 10.13304/j.nykjdb.2019.0406

小麦赤霉病生防菌的筛选及其田间防效研究

周红姿1,周方园1,赵晓燕1,吴翠霞2,张广志1,苑伟伟3,吴晓青1,谢雪迎1,范素素1,张新建1*

(1.齐鲁工业大学（山东省科学院）生态研究所, 山东省应用微生物重点实验室, 济南 250103；2.泰安市农业科学研究院, 山东泰安 271000；3.山东蔚蓝生物科技有限公司, 山东滨州 251700

收稿日期:2019-05-15 出版日期:2020-01-15 发布日期:2019-09-29
通讯作者: *通信作者张新建 E-mail：zhangxj@sdas.org
作者简介:周红姿 E-mail：zhouhz@sdas.org;
基金资助:
山东省科技发展计划项目（2017GSF21120）。

Screening of Biocontrol Agents Against Wheat Fusarium Head Blight and Its Field Control Experiment

ZHOU Hongzi1, ZHOU Fangyuan1, ZHAO Xiaoyan1, WU Cuixia2, ZHANG Guangzhi1, YUAN Weiwei3, WU Xiaoqing1, XIE Xueying1, FAN Susu1, ZHANG Xinjian1*

1.Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China; 2.Tai’an Academy of Agricultural Sciences, Shandong Tai’an 271000, China; 3.Shandong Vland Biotechnology Co., Ltd., Shandong Binzhou 251700, China

Received:2019-05-15 Online:2020-01-15 Published:2019-09-29

摘要/Abstract

摘要： 为了减少化学农药的使用并建立小麦赤霉病的高效生防技术，从各地小麦赤霉病发病穗部分离筛选到1株对小麦赤霉病具有高拮抗活性的细菌Z54，对该菌株进行菌株鉴定、室内抑菌测定、盆栽防病试验和田间小区防治试验。经分子生物学鉴定结合形态学分析，将该菌株鉴定为枯草芽胞杆菌（Bacillus subtilis）。平板对峙结果显示，Z54菌株对亚洲镰刀菌（Fusarium asiaticum）P2的菌丝抑制率达到70.13%；Z54菌株发酵液与亚洲镰刀菌P2在PDA上共培养结果显示，Z54菌株对P2的抑制率达到100%。盆栽防病试验显示，Z54菌株的菌体和菌株发酵液对小麦赤霉病的防治效果分别为78.57%和77.95%。田间小区试验显示，菌体与菌株发酵液对小麦赤霉病的防治效果分别为73.63%和75.82%。研究结果表明，枯草芽胞杆菌Z54菌株对亚洲镰刀菌引起的小麦赤霉病具有较好的防治效果，开发应用前景广阔。

关键词: 枯草芽胞杆菌, 亚洲镰刀菌, 小麦赤霉病, 生物防治

Abstract: In order to reduce the application of chemical fungicide and develop effective biological control of wheat Fusarium head blight, one bacterial strain named Z54 with high antagonistic activity against wheat Fusarium head blight was isolated and screened from the diseased wheat ear. Its strain identification, indoor bacteriostatic test and disease prevention test by pot and field experiments were carried out. Based on 16S rDNA sequencing, gyrB sequencing and morphological observation, strain Z54 was identified as a strain of Bacillus subtilis. Dual culture test showed that the inhibition rate against P2 strain of Fusarium asiaticum was 70.13%. Co-culture experiment of Z54 strain fermentation broth with F. asiaticum P2 on PDA showed that the inhibition rate of P2 was 100%. Pot test of wheat showed that the control rate of bacterial cells and fermentation broth of Z54 on wheat Fusarium head blight was 78.57% and 77.95%, respectively; the field test showed that the relative control rate was 73.63% and 75.82%, respectively. Present research showed that Z54 strain of B. subtilis had good control efficacy against wheat head blight caused by F. asiaticum, and broad prospects in development and application.

Key words: Bacillus subtilis, Fusarium asiaticum, wheat Fusarium head blight, biological control

周红姿1,周方园1,赵晓燕1,吴翠霞2,张广志1,苑伟伟3,吴晓青1,谢雪迎1,范素素1,张新建1*. 小麦赤霉病生防菌的筛选及其田间防效研究[J]. 中国农业科技导报, 2020, 22(1): 67-77.

ZHOU Hongzi1, ZHOU Fangyuan1, ZHAO Xiaoyan1, WU Cuixia2, ZHANG Guangzhi1, YUAN Weiwei3, WU Xiaoqing1, XIE Xueying1, FAN Susu1, ZHANG Xinjian1*. Screening of Biocontrol Agents Against Wheat Fusarium Head Blight and Its Field Control Experiment[J]. Journal of Agricultural Science and Technology, 2020, 22(1): 67-77.

[1]	王梦园1,杜延全2,蔡威威1,王子浩2,朱建强1. 复合菌剂防治草莓根腐病的效果研究[J]. 中国农业科技导报, 2020, 22(6): 100-110.
[2]	赵兴丽1，陶刚2,3，娄璇4，顾金刚5. 钩状木霉在辣椒根际定殖动态及其对辣椒疫病的生物防治[J]. 中国农业科技导报, 2020, 22(5): 106-114.
[3]	路露1,2,张孟丽2,狄怡琳2,朱凯1,石宝俊2. 百里香酚对不同时期秀丽线虫杀虫效果探究[J]. 中国农业科技导报, 2019, 21(9): 97-103.
[4]	吴晓青1,赵晓燕1,徐元章2,王加宁1,周方园1,周红姿1,张广志1,谢雪迎1,颜坤3,张新建1*. 植物生物防治精准化施药技术的研究进展[J]. 中国农业科技导报, 2019, 21(3): 13-21.
[5]	程亮1,2,郭青云1,2*. 燕麦镰刀菌GD\\|2菌株作为生物除草剂的潜力研究(英文)[J]. , 2014, 16(3): 70-80.
[6]	余小霞,田健,伍宁丰*. 枯草芽胞杆菌芽胞表面展示外源蛋白的研究进展[J]. , 2013, 15(5): 31-38.
[7]	邱德文. 生物农药与生物防治发展战略浅谈[J]. , 2011, 13(5): 88-92.
[8]	吕宁,王祺,宋娟,张一名,周洪友. 甜瓜采后病害生防菌遗传改良及其相关性状初步分析[J]. , 2011, 13(2): 25-30.
[9]	路雪君1,2,廖晓兰1,成飞雪2,刘勇2. 根结线虫的生物防治研究进展[J]. , 2010, 12(4): 44-48.
[10]	闫培生,曹立新,王凯,王琢. 真菌毒素生物防治研究进展[J]. , 2008, 10(6): 89-94.
[11]	宋晓妍孙彩云陈秀兰张玉忠. 木霉生防作用机制的研究进展[J]. , 2006, 8(6): 20-25.
[12]	江洪涛张红梅. 国内外水葫芦防治研究综述[J]. , 2003, 5(3): 72-75.
[13]	顾晓军田素芬. 发挥联合作用优势 \|实现害虫持续控制[J]. , 2002, 4(2): 60-65.

小麦赤霉病生防菌的筛选及其田间防效研究

Screening of Biocontrol Agents Against Wheat Fusarium Head Blight and Its Field Control Experiment

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics