铁皮石斛原球茎富硒悬浮培养条件优化

doi:10.13304/j.nykjdb.2020.1102

摘要/Abstract

摘要：

通过对铁皮石斛原球茎富硒悬浮培养的生长指标及多糖、生物碱、总蛋白质、硒含量等指标进行评价，优化铁皮石斛富硒悬浮培养条件，为开发富硒铁皮石斛产品提供理论依据与物质基础。研究结果表明，优化的铁皮石斛原球茎富硒悬浮培养基为MS+2.0 mg·L^-1 6-BA+ 0.50 mg·L^-1 NAA+30 g·L^-1蔗糖+0.05 mg·L^-1 Na₂SeO₃，接种量为100 g·L^-1，pH为5.8，继代周期为30 d，此时原球茎的硒含量最高，可达4.01 mg· kg^-1，多糖含量为34.79%、生物碱含量为0.026%、总蛋白质含量为28.25%。试验成功建立了富硒铁皮石斛原球茎悬浮培养体系。

关键词: 铁皮石斛, 富硒, 原球茎, 悬浮培养

Abstract:

In this study， the growth index， polysaccharide， alkaloid， total protein， selenium content and other indexes of Dendrobiumcandidum Procorms Se-enriched suspension culture were evaluated to optimize the conditions of Se-enriched suspension culture of Dendrobium candidum， providing theoretical basis and material basis for the development of Se-enriched Dendrobium candidum products. The results showed that the optimal Se-enriched suspension medium for protocorms of Dendrobium officinale was MS+2.0 mg·L^-1 6-BA+ 0.50 mg·L^-1 NAA+30g·L^-1 sucrose +0.05 mg·L^-1 Na₂SeO₃， inoculation amount was 100 g·L^-1， pH was 5.8. When the subculture period was 30 days， the selenium content of protocorms was up to 4.01 mg·kg^-1， polysaccharide content was 34.79%， alkaloid content was 0.026%， and total protein content was 28.25%. The suspension culture system of selenium-enriched suspension culture for protocorms of Dendrobium officinale was established successfully.

Key words: Dendrobium officinale, selenium-enriched, protocorm, suspension culture

中图分类号:

R282

韩素菊, 梅闯. 铁皮石斛原球茎富硒悬浮培养条件优化[J]. 中国农业科技导报, 2022, 24(4): 210-217.

Suju HAN, Chuang MEI. Conditions Optimization of Selenium-enriched Suspension Culture for Protocorms of Dendrobium officinale[J]. Journal of Agricultural Science and Technology, 2022, 24(4): 210-217.

图/表 9

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编号No.	6-BA/（mg·L^-1）	NAA/（mg·L^-1）	长势Growth	颜色Colour	疏松程度Looseness
1	0.0	0.00	-	逐渐褐化Go brown gradually	—
2	1.0	0.25	+	绿色Green	较紧密Dense
3	1.0	0.50	+	绿色Green	较紧密Dense
4	1.0	1.00	++	深黄绿色Dark chartreuse	较疏松Loose
5	2.0	0.25	+	绿色Green	较疏松Loose
6	2.0	0.50	+++	浅黄绿色Aqua	疏松Looser
7	2.0	1.00	++	黄绿色Chartreuse	较疏松Loose
8	2.5	0.25	+	绿色Green	较紧密Dense
9	2.5	0.50	+	绿色Green	较紧密Dense
10	2.5	1.00	+	深绿色Dark green	紧密Denser

编号No.	6-BA/（mg·L^-1）	NAA/（mg·L^-1）	长势Growth	颜色Colour	疏松程度Looseness
1	0.0	0.00	-	逐渐褐化Go brown gradually	—
2	1.0	0.25	+	绿色Green	较紧密Dense
3	1.0	0.50	+	绿色Green	较紧密Dense
4	1.0	1.00	++	深黄绿色Dark chartreuse	较疏松Loose
5	2.0	0.25	+	绿色Green	较疏松Loose
6	2.0	0.50	+++	浅黄绿色Aqua	疏松Looser
7	2.0	1.00	++	黄绿色Chartreuse	较疏松Loose
8	2.5	0.25	+	绿色Green	较紧密Dense
9	2.5	0.50	+	绿色Green	较紧密Dense
10	2.5	1.00	+	深绿色Dark green	紧密Denser

接种量 Inoculating quantity/g	鲜重 Fresh weight/g	干重 Dry weight/g	增殖系数 Multiplication
1	1.42±0.62	0.18±0.06	0.42±0.36 d
2	3.19±0.36	0.38±0.02	0.59±0.86 b
3	5.07±0.83	0.49±0.11	0.69±0.13 a
4	6.54±1.31	0.59±0.31	0.63±0.45 b
5	7.65±1.43	0.62±0.43	0.53±0.06 c

接种量 Inoculating quantity/g	鲜重 Fresh weight/g	干重 Dry weight/g	增殖系数 Multiplication
1	1.42±0.62	0.18±0.06	0.42±0.36 d
2	3.19±0.36	0.38±0.02	0.59±0.86 b
3	5.07±0.83	0.49±0.11	0.69±0.13 a
4	6.54±1.31	0.59±0.31	0.63±0.45 b
5	7.65±1.43	0.62±0.43	0.53±0.06 c

培养天数 Cultivated days/d	鲜重 Fresh weight/g	干重 Dry weight/g	增殖系数 Multiplication
6	5.66±0.15	0.51±0.17	0.89±0.13 i
9	7.64±0.32	0.65±0.21	1.55±0.24 h
12	9.38±0.05	0.69±0.13	2.13±0.09 g
15	10.13±1.00	0.71±0.15	2.38±0.54 e
18	10.33±1.13	0.72±0.08	2.44±0.31 d
21	10.76±1.00	0.79±0.18	2.59±0.41 c
24	10.95±2.55	0.83±0.14	2.65±0.22 b
27	11.06±1.88	0.85±0.26	2.69±0.18 b
30	11.26±1.67	0.88±0.08	2.75±0.41 a
33	11.59±1.52	0.89±0.11	2.86±0.08 a
36	11.52±1.77	0.89±0.09	2.84±0.54 a
39	10.84±1.20	0.89±0.66	2.61±0.41 b
42	10.70±1.41	0.88±0.29	2.57±0.33 c
45	10.58±1.31	0.88±0.43	2.53±0.71 c
48	10.55±1.53	0.87±0.44	2.52±0.27 c
51	9.84±1.41	0.87±0.12	2.28±0.11 f