作物重茬连作障碍中自毒物质的研究进展

doi:10.13304/j.nykjdb.2021.0889

摘要/Abstract

摘要：

在设施农业的大背景下，连作障碍普遍存在于粮食作物、经济作物、蔬菜、林木及中药材等各种植物中，严重制约了种植业的可持续发展。大量研究表明自毒作用是导致重茬连作障碍的主要原因之一，系统介绍了自毒物质的来源和种类、作用特点及机理，概述了自毒物质的提取、活性检测、降解功能菌株分离筛选等研究方法，系统归纳了土壤改良、耕作模式改良、生物防治等消减连作障碍的措施，并提出了现有研究中存在的问题和今后发展重点，为自毒作用的深入研究提供新思路。

关键词: 连作障碍, 自毒物质, 自毒作用

Abstract:

Under the background of facility agriculture， replant disease is widespread in grain crops， such as food crops， cash crops， vegetables， trees， Chinese herbal medicines and other plants， which seriously restricts the sustainable development of planting industry. A large number of studies have confirmed that autotoxicity is one of the main causes of replant disease， so the paper systematically introduced the sources， types， characteristics and mechanism of autotoxicity， summarized research methods such as extraction of autotoxic substances， activity detection， isolation and screening of functional degradation strains， and analyzed the abatement measures such as soil improvement， cultivation mode improvement， biological control and others. As well as， the problems existing in the research and development research emphasis in the future were putted forward. It would provide reference for the in-depth study of replant disease and technical support for the green and healthy development of facility agriculture.

Key words: replant disease, autotoxins, autotoxicity

中图分类号:

陈福慧, 申乃坤, 姜明国, 王一兵. 作物重茬连作障碍中自毒物质的研究进展[J]. 中国农业科技导报, 2022, 24(10): 125-132.

Fuhui CHEN, Naikun SHEN, Mingguo JIANG, Yibing WANG. Research Progress of Autotoxic Secretions in Crops Replant Successive Cropping Obstacles[J]. Journal of Agricultural Science and Technology, 2022, 24(10): 125-132.

参考文献 59

1	BLOK W J, BOLLEN G J. The role of autotoxins from root residues of the previous crop in the replant disease of asparagus [J]. Neth. J. Plant Pathol., 1993, 99(3): 29-40.
2	邵财.人参连作障碍化感作用研究[D].北京:中国农业科学院,2009.
	SHAO C. Study on allelopathy in continuous cropping obstacle of Panax ginseng [D]. Beijing: Chinese Academy of Agricultural Sciences, 2009.
3	唐建军,项田夫,张禄源,等.植物次生代谢、离体培养条件下次生代谢物积累及其调控研究进展[J].中国野生植物资源,1998,17(4):3-8.
	TANG J J, XIANG T F, ZHANG L Y, et al.. Research progress on plant secondary metabolism, accumulation and regulation of secondary metabolites in plant culture in vitro. Chin [J]. Wild Plant Resour., 1998, 17(4): 3-8.
4	陶茸.香豆素、咖啡酸对紫花苜蓿及轮作作物幼根形态和结构的影响及其生理变化[D].兰州:甘肃农业大学,2019.
	TAO R. Effects of coumarin and caffeic acid on morphological and structure of young roots of Alfalfa and rotation crops and their physiological changes [D]. Lanzhou: Gansu Agricultural University, 2019.
5	张璐.连作甜瓜土壤中酚酸类物质的变化规律和木霉菌降解作用研究[D].沈阳:沈阳农业大学,2017.
	ZHANG L. Determination change rules of phenolic acids in the rhizosphere soil of replanted muskmelon and degradation of trichoderma [D]. Shenyang: Shenyang Agricultural University, 2017.
6	原增艳.地黄自毒物质的分离与鉴定[D].郑州:河南农业大学,2010.
	YUAN Z Y. Isolation and identification of autotoxicity substances from Rehmannia glutinosa libosch [D]. Zhengzhou: Henan Agricultural University, 2010.
7	黄玉茜,杨劲峰,梁春浩,等.香草酸对花生种子萌发、幼苗生长及根际微生物区系的影响[J].中国农业科学,2018,51(9):1735-1745.
	HUANG Y Q, YANG J F, LIANG C H, et al.. Effects of vanillic acid on seed germination, seedling growth and rhizosphere microflora of peanut [J]. Sci. Agric. Sin., 2018, 51(9):1735-1745.
8	AURORA L N, SOBEIDA S N, ANA L A, et al.. Phytotoxic effects of Sicyos deppei (Cucurbitaceae) in germinating tomato seeds [J]. Physiol. Plant., 2009, 136(2):180-192.
9	林文雄,陈婷,周明明.农业生态学的新视野[J].中国生态农业学报,2012,20(3):253-264.
	LIN W X, CHEN T, ZHOU M M. New dimensions in agroecology [J]. Chin. J. Eco-Agric., 2012, 20(3): 253-264.
10	叶素芬.黄瓜根系自毒物质对其根系病害的助长作用及其缓解机制研究[D].杭州:浙江大学,2004.
	YE S F. Research on promotive effects of fusarium wilt in cucumis sativus by cinnamic acid, an autotoxin in root exudates of Cucumis sativus L., and mitigation mechanism by grafting and cinnamic acid-degrading microbial strains. [D]. Hangzhou: Zhejiang University, 2004.
11	龚静,马海艳,郑顺林,等.连作对马铃薯酚酸类自毒物质及根际真菌群落的影响[J].西北农业学报,2021,30(3):431-438.
	GONG J, MA H Y, ZHENG S L, et al.. Effect of continuous cropping on autotoxic substances and rhizosphere fungi communities in potato [J]. Acta Agric. Bor-Occid. Sin., 2021, 30(3):431-438.
12	黄玉茜,韩立思,杨劲峰,等.花生植株和土壤水浸液自毒作用研究及土壤中自毒物质检测[J].生态学报,2012,32(19):6023-6032.
	HUANG Y Q, HAN L S, YANG J F, et al.. Autotoxicity of aqueous extracts from plant, soil of peanut and identification of autotoxic substances in rhizospheric soil [J]. Acta Ecol. Sin., 2012, 32(19):6023-6032.
13	尹玉玲,周劲松,汤泳萍,等.芦笋连作障碍中的自毒物质研究进展[J].生态科学,2019,38(5):204-209.
	YIN Y L, ZHOU J S, TANG Y P, et al.. Review on autotoxicity of asparagus (Asparagus officinalis L.) in continuous cropping system [J]. Ecol. Sci., 2019, 38(5):204-209.
14	颜仕龙,蔡波,唐华玲,等.苦瓜不同部位化感作用研究[J].中国农业科技导报,2018,20(8):100-107.
	YAN S L, CAI B, TANG H L, et al.. Allelopathy of different parts of Momordica charantia [J]. J. Agric. Sci. Technol., 2018, 20(8):100-107.
15	马祥庆,刘爱琴,黄宝龙.杉木人工林自毒作用研究[J].南京林业大学学报,2000,24 (1):15-19.
	MA X Q, LIU A Q, HUANG B L. A study on self-poisoning effects of Chinese fir plantation [J]. J. Nanjing For. Univ., 2000, 24 (1):15-19.
16	向维.三七根系分泌物的自毒作用及自毒物质研究[D].南宁:广西大学,2016.
	XIANG W. Autotoxicity in Panax notoginseng of root exudates and their allelochemicals [D]. Nanning: Guangxi University, 2016.
17	杨广超,吕卫光,朱静,等.西瓜根、茎、叶水浸提液对西瓜种子萌发及幼苗中酶活性的影响[J].西北农业学报,2005,14 (1):46-51.
	YANG G C, LYU W G, ZHU J, et al.. Effects of water abstracts of watermelon’s root, stem and leaf on seed germination and enzyme activities of seedlings [J]. Acta Agric. Bor-Occid. Sin., 2005, 14 (1):46-51.
18	李敏,闫兴富,马丽,等.酚酸类化感自毒物质对枸杞种子萌发的抑制作用[J].生态学报,2020,40(6):2072-2079.
	LI M, YAN X F, MA L, et al.. Allelopathic inhibition of phenolic acids on germination of wolfberry ( Lycium barbarum Linn.) [J]. Acta Ecol. Sin., 2020, 40(6):2072-2079.
19	王倩,李晓林.苯甲酸和肉桂酸对西瓜幼苗生长及枯萎病发生的作用[J].中国农业大学学报,2003,8(1):83-86.
	WANG Q, LI X L. Effects of benzoic and cinnamic acids on watermelon seedling growth and Fusarium wilt occurrence [J]. J. China Agric. Univ., 2003, 8 (1):83-86.
20	PENUELAS J, LLUSIA J. Effects of carbon dioxide, water supply, and seasonality on terpene content and emission by Rosmarinus officinalis [J]. J. Chem. Ecol., 1997, 23(4):979-993.
21	李丹,秦伟英,刘正坪,等.西瓜根系分泌物对西瓜枯萎病菌及其生防菌的化感作用[C]//彭友良,李向东.中国植物病理学会2017年学术年会论文集.北京:中国农业科学技术出版社, 2017:468.
	LI D, QIN W Y, LIU Z P, et al.. Allelopathic effects of watermelon root exudates on Fusarium wilt and its biocontrol bacteria [C]//PENG Y L, LI X D. Proceedings of the Ann. Sual Meeting of Chinese society of Plant Pathology. Beijing: China Agricultural Science and Technology Press, 2017:468.
22	杨航,祁娟,刘文辉,等.异龄老芒麦根际土壤中脂肪酸类物质检测及根际土壤的自毒效应研究[J].草地学报,2021,29(1):52-59.
	YANG H, QI J, LIU W H, et al.. Fatty acid substance detection and self-toxic effect in rhizosphere soil of Elymus sibiricus L. with different planting years [J]. Acta Agrestia Sin., 2021, 29(1):52-59.
23	喻景权,杜尧舜.蔬菜设施栽培可持续发展中的连作障碍问题[J].沈阳农业大学学报,2000,31(1):124-126.
	YV J Q, DU Y S. Soil-sickness problem in the sustainable development for the protected production of vegetables [J]. J. Shenyang Agric. Univ., 2000, 31 (1):124-126.
24	蒋红云,张燕宁,冯平章,等.石蒜对萝卜、黄瓜、番茄和油菜幼苗的化感效应[J].应用生态学报,2006,17(9):1655-1659.
	JIANG H Y, ZHANG Y N, FENG P Z, et al.. Allelopathic effects of Lycoris radiate on radish, cucumber, tomato and rape seedlings [J]. Chin. J. Appl. Ecol., 2006, 17(9):1655-1659.
25	王芳.茄子连作障碍机理研究[D].北京:中国农业大学,2003.
	WANG F. The mechanism of eggplant (Solanum melongena L.) replanting problem [D]. Beijing: China Agricultural University, 2003.
26	章黎黎,李兴武.玉米不同部位水浸提液对绞股蓝种子的化感作用研究[J].种子,2018,37(8):79-82, 87.
	ZHANG L L, LI X W. Study on allelopathy of different parts of maize aqueous extracts on Gynostemma pentaphyllum seeds [J]. Seed, 2018, 37(8):79-82, 87.
27	郭凯,燕志强,金辉,等.肉桂酸和咖啡酸对莴苣生长的化感作用及其机理研究[J].西北植物学报,2016,36(1):93-99.
	GUO K, YAN Z Q, JIN H, et al.. Allelopathic effect and mechanism of cinnamic acid and caffeic acid on the growth of lettuce [J]. Acta Bot. Bor-Occid. Sin., 2016, 36(1):93-99.
28	王璞,赵秀琴.几种化感物质对棉花种子萌发及幼苗生长的影响[J].中国农业大学学报,2001,6(3):26-31.
	WANG P, ZHAO X Q. Effect of allelochemicals on cotton seed germination and seedling growth [J]. J. China Agric. Univ., 2001, 6(3):26-31.
29	BLUM U, GERIG T M, WORSHAM A D, et al.. Modification of allelopathic effects of p-coumaric acid on morning-glory seedling biomass by glucose, methionine, and nitrate [J]. J. Chem. Ecol., 1993, 19(12):2791-2811.
30	BAZIRAMAKENGA R, LEROUX G D, SIMARD R R. Effects of benzoic and cinnamic acids on membrane permeability of soybean roots [J]. J. Chem. Ecol., 1995, 21(9):1271-1285.
31	刘苹,赵海军,万书波,等.花生根系分泌物自毒作用研究[J].中国油料作物学报,2010,32(3):431-435.
	LIU P, ZHAO H J, WAN S B, et al.. Autotoxic potential of peanut (Arachis hypogaea) root exudates [J]. Chin. J. Oil Crop Sci., 2010, 32(3):431-435.
32	李冠喜,吴小芹,叶建仁.杨树根际土自毒物质的积累、毒害及生物修复[J].南京林业大学学报(自然科学版),2013,37(3):71-76.
	LI G X, WU X Q, YE J R. Accumulation, toxic properties and bioremediation of autotoxic substance in poplar rhizosphere soil [J]. J. Nanjing For. Univ. (Nat. Sci.), 2013, 37(3):71-76.
33	LIU D L, LOVETT J V. Biologically active secondary metabolites of barley. Ⅱ. phytotoxicity of barley allelochemicals [J]. J. Chem. Ecol., 1993, 19(10):2231-2244.
34	CRUZ-ORTEGA R, ANAYA A L, HERNANDEZ-BAUTISTA B E, et al.. Effects of allelochemical stress produced by Sicyos deppei on seedling root ultrastructure of Phaseolus vulgaris and Cucurbita ficifolia [J]. J. Chem. Ecol., 1998, 24(12):2039-2057.
35	BARKOSKY R R, BUTLER J L, EINHELLIG F A. Mechanisms of hydroquinone-induced growth reduction in Leafy spurge [J]. J. Chem. Ecol., 1999, 25(7):1611-1621.
36	ALSAADAWI S I, AL-HADITHY M S, ARIF B M. Effects of three phenolic acids on chlorophyll content and ions uptake in cowpea seedlings [J]. J. Chem. Ecol., 1986, 12(1):221-227.
37	HOLAPPA L D, BLUM U. Effects of exogenously applied ferulic acid, a potential allelopathic compound, on leaf growth, water utilization, and endogenous abscisic acid levels of tomato, cucumber, and bean [J]. J. Chem. Ecol., 1991, 17(5):865-886.
38	李振方,齐晓辉,李奇松,等.地黄自毒物质提取及其生物指标测定[J].生态学报,2010,30(10):2576-2584.
	LI Z F, QI X H, LI Q S, et al.. Extraction, bioassay, and chemical identification of Rehmannia glutinosa L. autotoxins [J]. Acta Ecol. Sin., 2010, 30(10):2576-2584.
39	HEJI A A, EINHELLIG F A, RASMUSSEN J A. Effects of juglone on growth, photosynthesis, and respiration [J]. J. Chem. Ecol., 1993, 19(3):559-568.
40	周凯,郭维明,王智芳,等.菊花不同部位及根际土壤水浸液处理对光合作用的自毒作用研究[J].中国生态农业学报,2009,17(2):318-322.
	ZHOU K, GUO W M, WANG Z F, et al.. Autotoxicity of aquatic extracts from Chrysanthemum and rhizosphere soil on photosynthesis in the same plant species [J]. Chin. J. Eco-Agric., 2009, 17(2):318-322.
41	YANG Q M, LI Z N, ZHOU C H. Effects of three allelopathic phenolics on chlorophyll accumulation of rice (Oryza sativa) seedlings: I. inhibition of supply-orientation [J]. Bot. Bull. Acad. Sin., 2002, 43(4):299-304.
42	林茂兹,张志兴,林争春,等.太子参连作障碍蛋白差异表达分析[J].草业学报,2010,19(6):197-207.
	LIN M Z, ZHANG Z X, LIN Z C, et al.. Analysis of differential expression of proteins in replanting disease of Pseudostellaria heterophylla [J]. Acta Pratac. Sin., 2010, 19(6):197-207.
43	BAZIRAMAKENGA R, LEROUX G D, SIMARD R R, et al.. Allelopathic effects of phenolic acids on nucleic acid and protein levels in soybean seedlings [J]. NRC Res., 1997, 75(3):445-450.
44	陈长宝,刘继永,王艳艳,等.人参根际化感作用及其对种子萌发的影响[J].吉林农业大学学报,2006,28(5):534-537, 541.
	CHEN C B, LIU J Y, WANG Y Y, et al.. Allelopathy of ginseng rhizosphere and its effect on germination of seed [J]. J. Jilin Agric. Univ., 2006, 28(5):534-537, 541.
45	李勇,黄小芳,丁万隆,等.不同土壤提取物对人参种子生长的化感效应及其化学组成[J].生态环境,2008,17(3):1173-1178.
	LI Y, HUANG X F, DING W L, et al.. Allelopathic effects of soil extracts on the growth of ginseng seeds and its chemical composition [J]. Ecol. Environ., 2008, 17(3):1173-1178.
46	ASADUZZAMAN M, ASAO T. Autotoxicity in beans and their allelochemicals [J]. Sci. Hortic., 2011, 134(1):26-31.
47	周家明,崔秀明,曾鸿超,等.三七根系分泌物的化学成分研究[J].特产研究,2009,31(3):37-9.
	ZHOU J M, CUI X M, ZENG H C, et al.. Study on chemical composition of root exudates of Panax notoginseng [J]. Special Wild Econ. Anim. Plant Res., 2009, 31(3):37-39.
48	曾任森,林象联,骆世明,等.蟛蜞菊的生化他感作用及生化他感作用物的分离鉴定[J].生态学报,1996,10(1):20-27.
	ZENG R S, LIN X L, LUO S M, et al.. Allelopathic potential of Wedelia chinensis and its allelochemicals [J]. Acta Ecol. Sin., 1996, 10(1):20-27.
49	吴锦兰,魏希颖.防治草莓重茬病多功能内生菌株的筛选及鉴定[J].陕西师范大学学报(自然科学版),2021,49(1):105-111.
	WU J L, WEI X Y. Screening and identification of multifunctional endophytic strains for prevention and control of strawberry replant disease [J]. J. Shaanxi Norm. Univ. (Nat. Sci.), 2021, 49(1):105-111.
50	石瑞,张亚琪,冯玉萍,等.高粱自毒物质降解细菌的筛选[J].中国饲料,2020,7(1):28-31.
	SHI R, ZHANG Y Q, FENG Y P, et al.. Screening of bacteria for degradation of autotoxic substances in sorghum [J]. China Feed, 2020, 7(1):28-31.
51	王罗涛,杨冬英,邓琳梅,等.三七根际土壤中皂苷类自毒物质降解拮抗细菌的分离筛选[J].南方农业学报,2020,51(2):305-312.
	WANG L T, YANG D Y, DENG L M, et al.. Isolation and screening of antagonistic autotoxin-degrading bacteria in Panax notoginseng (Burk.) F.H.Chen rhizosphere soil [J]. J. South Agric., 2020, 51(2):305-312.
52	唐爽爽,张照然,马周杰,等.甜瓜自毒物质降解细菌的筛选及发酵条件优化研究[C]//彭友良,王文明,陈学伟.中国植物病理学会2019年学术年会论文集.北京: 中国农业科学技术出版社, 2019:361.
	TANG S S, ZHANG Z R, MA Z J, et al.. Screening of muskmelon autotoxic degrading bacteria and optimization of fermentation conditions [C]//PENG Y L,WANG W M, CHEN X W. Proceedings of the Annual Meeting of Chinese society of Plant Pathology. Beijing: China Agricultural Science and Technology Press, 2019:361.
53	张晓玲,潘振刚,周晓锋,等.自毒作用与连作障碍[J].土壤通报,2007,38(4):781-784.
	ZHANG X L, PAN Z G, ZHOU X F, et al.. Autotoxicity and continuous cropping obstacles: a review [J]. Chin. J. Soil Sci., 2007, 38(4):781-784.
54	王宁,张锴,郭建明,等.土壤消毒技术在国外草莓栽培中的应用综述[J].现代农业科技,2014,12(1):143-144, 146.
	WANG N, ZHANG K, GUO J M, et al.. Application summary of soil fumigation in strawberry cultivation abroad [J]. Mod. Agric. Sci. Technol., 2014, 12(1):143-144, 146.
55	CHEN B, YUAN M, QIAN L. Enhanced bioremediation of PAH-contaminated soil by immobilized bacteria with plant residue and biochar as carriers [J]. J. Soils Sediments, 2012, 12(9):1350-1359.
56	欧小宏,金航,郭兰萍,等.平衡施肥及土壤改良剂对连作条件下三七生长与产量的影响[J].中国中药杂志,2012,37(13):1905-1911.
	OU X H, JIN H, GUO L P, et al.. Effects of balanced fertilization and soil amendment on growth and yield of Sanqiin continuous cropping [J]. China J. Chin. Mater. Med., 2012, 37(13):1905-1911.
57	吴红淼,吴林坤,王娟英,等.根际调控在缓解连作障碍和提高土壤质量中的作用和机理[J]. 生态科学,2016,35(5):225-232.
	WU H M, WU L K, WANG J Y, et al.. The mechanisms of the rhizosphere management on the remission in consecutive monoculture problem and the improvement of soil quality [J]. Ecol. Sci., 2016, 35(5):225-232.
58	喻国辉,谢银华,陈燕红,等.利用微生物缓解苯丙烯酸对黄瓜生长的抑制[J].微生物学报,2006,46(6):934-938.
	YV G H, XIE Y H, CHEN Y H, et al.. Mitigating the repress of cinnamic acid to cucumber growth by microbial strain [J]. Acta Microbiol. Sin., 2006, 46(6):934-398.
59	李敏,张丽叶,张艳江,等.酚酸类自毒物质微生物降解转化研究进展[J].生态毒理学报,2019,14(3):72-78.
	LI M, ZHANG L Y, ZHANG Y J, et al.. Review on the microbial biodegradation and metabolism of autotoxic phenolic acids [J]. Asian J. Ecotoxicol., 2019, 14(3):72-78.

[1]	杨莉, 于俐, 孙卓, 张桐毓, 张阳, 杨利民. 人参根系分泌物中有机酸及皂苷对人参病原菌与生防菌的化感差异研究[J]. 中国农业科技导报, 2022, 24(6): 145-155.
[2]	李浩成1，2，左应梅2，杨绍兵2，杨天梅2，李纪潮2，杨维泽2，张金渝2*. 三七根系分泌物在连作障碍中的生态效应及缓解方法[J]. 中国农业科技导报, 2020, 22(8): 159-167.
[3]	李庆凯1,2,3,刘苹2,3,赵海军3,宋效宗2,林海涛2,沈玉文2,李林1,万书波1,3. 玉米根系分泌物对连作花生土壤酚酸类物质化感作用的影响[J]. 中国农业科技导报, 2020, 22(3): 119-130.
[4]	毛宁1,薛泉宏1,唐明2,王玲娜3,赵娟3,段春梅1. 放线菌对对羟基苯甲酸的降解作用及草莓生长的影响[J]. , 2010, 12(5): 103-108.
[5]	张翠绵1,李洪涛1,李晓芝2,贾楠1,胡栋1,王占武1. 链霉菌S506对设施黄瓜根际生态和生产性状的影响[J]. , 2010, 12(5): 98-102.
[6]	张子龙,王文全. 药用植物连作障碍的形成机理及其防治[J]. , 2009, 11(6): 19-23.
[7]	薛泉宏,同延安. 土壤生物退化及其修复技术研究进展[J]. , 2008, 10(4): 28-35.
[8]	阮维斌,王敬国,张福锁,申建波. 根际微生态系统理论在连作障碍中的应用[J]. , 1999, 1(4): 53-58.
[9]	段玉琪1,陈冬梅2,晋艳1,王海斌2,杨宇虹1,尤垂淮2,田卫霞2,林文雄2. 不同肥料对连作烟草根际土壤微生物及酶活性的影响[J]. , 1, 1(1): 122-126.