人参根系分泌物中有机酸及皂苷对人参病原菌与生防菌的化感差异研究

doi:10.13304/j.nykjdb.2021.0303

摘要/Abstract

摘要：

采用离体培养法探讨了人参根系分泌物中有机酸类物质（苯甲酸、邻苯二甲酸、己二酸、丁二酸）及皂苷类物质（人参皂苷Rb2、Rd、Rc、Rg1、Re）对人参主要病原菌及其生防菌的化感作用差异。结果显示，有机酸及皂苷对微生物的化感作用表现出受体多、阈值低（1 μg·L^-1）、作用质量浓度范围大（1 μg·L^-1~1 mg·L^-1），但无明显浓度效应的特点；有机酸及皂苷的化感作用强度介于-132.94%~31.33%，对生防菌的促进作用大于病原菌，对部分病原菌生长具有直接的抑制活性，二者对病原菌与生防菌表现出一定的偏害/偏利作用；人参皂苷Rd、Re具有抑制病原菌、促进生防菌的作用，是人参根系分泌物中重要的萜类防御物质。

关键词: 连作障碍, 病原菌, 生态功能, 偏利作用, 根际微生态

Abstract:

To investigate the allelopathic effects of ginseng root exudates on pathogenic bacteria and biocontrol bacteria of pathogenic bacteria， the allelopathic effects of organic acids （benzoic acid， phthalic acid， adipic acid， succinic acid） and saponins （ginsenoside Rb2， Rd， Rc Rg1， Re） in root exudates of Panaxginseng on these microorganisms were studied in vitro. The results showed that the allelopathic effects of organic acids and saponins on microorganisms were characterized by multiple receptors， low threshold （1 μg·L^-1）， wide concentration range （1 μg·L^-1~1 mg·L^-1）， but no obvious concentration effect. Allelopathy of organic acids and saponins ranged from -132.94% to 31.33%， the promotion effect of these substances on biocontrol bacteria was greater than that on pathogenic bacteria， and the direct inhibitory activity on the growth of some pathogenic bacteria was observed， these metabolites separated from ginseng root exudates showed certain harmful/beneficial effects on pathogenic bacteria and biocontrol bacteria. Ginsenosides Rd and Re had the function of inhibiting pathogenic bacteria and promoting biocontrol bacteria， which meant they were the important terpenoid defense substances in root exudates of ginseng.

Key words: continuous cropping obstacle, pathogen, ecological function, preferential effect, rhizosphere microecology

中图分类号:

S476

杨莉, 于俐, 孙卓, 张桐毓, 张阳, 杨利民. 人参根系分泌物中有机酸及皂苷对人参病原菌与生防菌的化感差异研究[J]. 中国农业科技导报, 2022, 24(6): 145-155.

Li YANG, Li YU, Zhuo SUN, Tongyu ZHANG, Yang ZHANG, Limin YANG. Allelopathic Effects of Organic Acids and Saponins in Ginseng Root Exudates on Pathogenic and Biocontrol Bacteria[J]. Journal of Agricultural Science and Technology, 2022, 24(6): 145-155.

图/表 6

参考文献 34

1	吴林坤, 林向民, 林文雄. 根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望[J]. 植物生态学报, 2014, 38(3): 298-310.
	WU L K, LIN X M, LIN W X. Advances and perspective in research on plant-soil-microbe interactions mediated by root exudates [J]. Chin. J. Plant Ecol., 2014,38(3): 298-310.
2	艾超, 孙静文, 王秀斌, 等. 植物根际沉积与土壤微生物关系研究进展[J]. 植物营养与肥料学报, 2015,21(5): 1343-1351.
	AI C, SUN J W, WANG X B, et al.. Advances in the study of the relationship between plant rhizodeposition and soil microorganism [J]. Plant Nutr. Fert. Sci.,2015, 21(5): 1343-1351.
3	张重义, 陈慧, 杨艳会, 等. 连作对地黄根际土壤细菌群落多样性的影响[J]. 应用生态学报, 2010, 21(11): 2843-2848.
	ZHANG C Y, CHEN H, YANG Y H, et al.. Effects of continuous cropping on bacterial community diversity in rhizosphere soil of Rehmannia glutinosa [J]. Chin. J. Appl. Ecol., 2010, 21(11): 2843-2848.
4	BULGARELLI D, SCHLAEPPI K, SPAEPEN S,et al.. Structure and function of the bacterial microbiota of plants [J]. Annu. Rev. Plant Biol., 2013, 64 (1): 807-838.
5	陈宏宇, 李晓鸣, 王敬国. 抗病性不同大豆品种根面及根际微生物区系的变化Ⅰ.非连作大豆(正茬)根面及根际微生物区系的变化[J]. 植物营养与肥料学报, 2005(6): 98-103.
	CHEN H Y, LI X M, WANG J G. Change of microflora in the rhizoplane and rhizosphere of different disease resistance soybean cultivars Ⅰ. change of microflora in the rhizoplane and rhizosphere of soybean under normal rotation cropping condition [J]. Plant Nutr. Fert. Sci., 2005(6): 98-103.
6	FONS F, AMELLAL N, LEYVAL C, et al.. Effects of gypsophila saponins on bacterial growth kinetics and selection of subterranean clover rhizosphere bacteria [J]. Can. J. Microbiol., 2003, 49(6): 367-373.
7	吴林坤, 黄伟民, 王娟英, 等. 不同连作年限野生地黄根际土壤微生物群落多样性分析[J]. 作物学报, 2015, 41(2): 308-317.
	WU L K, HUANG W M, WANG J Y, et al.. Diversity analysis of rhizosphere microflora of Wild R. glutinosa grown in monocropping for different years [J]. Acta Agron. Sin., 2015, 41(2): 308-317.
8	孔垂华, 娄永根. 化学生态学前沿[M]. 北京: 高等教育出版社,2010:1-63.
9	孙浩, 黄璐明, 黄璐琦, 等. 基于生态位理论的药用植物化感作用与连作障碍的探讨[J]. 中国中药杂志, 2008, 33(17):2197-2200.
	SUN H, HUANG L M, HUANG L Q, et al..Study on medicinal plant allelopathy and soil sickness based on ecological niche [J]. China J. Chin. Materia Medica, 2008, 33(17): 2197-2200.
10	HÄTTENSCHWILER S, VITOUSEK P M. The role of polyphenols in terrestrial ecosystem nutrient cycling [J]. Trends Ecol. Evol., 2000, 15(6): 238-243.
11	INDERJIT, DAVID A W, RICHARD K, et al.. The ecosystem and evolutionary contexts of allelopathy [J]. Trends Ecol. Evol., 2011, 26(12): 655-662.
12	陈爱国, 李明杰, 张宝, 等. 连作介导的药用植物及其根际微生态灾变机制研究展望[J]. 中国现代中药, 2016, 18(2): 239-245.
	CHEN A G, LI M J, ZHANG B, et al.. Review on catastrophe mechanism of medicinal plant and its rhizosphere microecosystem mediated by consecutive monoculture [J]. Mod. Chin. Med., 2016, 18(2): 239-245.
13	杨利民. 中药材生态种植理论与技术前沿[J].吉林农业大学学报, 2020, 42(4): 355-363.
	YANG L M. Theory and technology frontiers of ecological planting of chinese medicinal materials [J]. J. Jilin Agric. Univ., 2020, 42(4): 355-363.
14	雷锋杰, 张爱华, 张秋菊, 等. 人参、西洋参化感作用研究进展[J].中国中药杂志, 2010, 35(17): 2221-2226.
	LEI F J, ZHANG A H, ZHANG Q J, et al.. Advances in allelopathy of Panax ginseng and Panax quinquefolium [J]. China J. Chin. Materia Medica, 2010,35(17): 2221-2226.
15	孙卓. 人参病害生防细菌的筛选及其防病作用研究[D]. 长春:吉林农业大学, 2014.
	SUN Z. Screening of biocontrol bacteria against ginseng diseases [D]. Changchun: Jilin Agricultural University, 2014.
16	杨莉, 任晶, 韩梅, 等. 人参根系分泌物中酸性物质的化感活性与互作效应[J]. 吉林农业大学学报, 2017, 39(5): 570-574 .
	YANG L, REN J, HAN M, et al.. Allelopathy and interaction of acidic materials in ginseng root exudates [J]. J. Jilin Agric.Univ., 2017, 39(5): 570-574.
17	任晶. 人参根际有机酸的化感活性及环境行为研究[D].长春:吉林农业大学, 2016.
	REN J. Allelopathic activity and environmental behavior of organic acids in ginseng rhizosphere [D]. Changchun: Jilin Agricultural University, 2016.
18	LATIF S, GENEVIÈVE C, WESTON L A. Allelopathy and the role of allelochemicals in plant defence [J]. Adv. Botanical Res., 2017, 82:19-54.
19	LIU J, LI X, JIA Z, et al.. Effect of benzoic acid on soil microbial communities associated with soilborne peanut diseases [J]. Appl. Soil Ecol., 2016, 110 (Complete):34-42.
20	周柳婷, 罗扬, 李建鹃, 等. 酚酸介导下连栽木麻黄根际微生物变化[J]. 生态学杂志, 2021, 40(4): 1021-1028.
	ZHOU L T, LUO Y, LI J J, et al.. The variation of rhizosphere microorganisms of replanted Casuarina equisetifolia plantations mediated by phenolic acids [J]. Chin. J. Ecol., 2021,40(4): 1021-1028.
21	孙香荣, 李娟, 钮颜宇, 等. 基于膜技术的地黄根区土壤化感物质分离效果研究[J]. 河南农业大学学报, 2021,55(1): 35-43.
	SUN X R, LI J, NIU Y Y, et al.. Study on the separation effect of soil allelochemicals in Rehmannia glutinosa root zone based on membrane technology [J]. J. Henan Agric.Univ., 2021, 55(1): 35-43.
22	刘芳君. 无土栽培人参根系分泌物的化感作用研究[D]. 长春:吉林农业大学, 2014.
	LIU F J. Allelopathy of root exudates from soilless cultivated Ginseng [D]. Changchun: Jilin Agricultural University, 2014.
23	吴红淼, 林文雄. 药用植物连作障碍研究评述和发展透视[J]. 中国生态农业学报, 2020, 28(6): 775-793.
	WU H M, LIN W X.A commentary and development perspective on the consecutive monoculture problems of medicinal plants [J]. Chin. J. Eco-Agric., 2020, 28(6): 775-793.
24	李浩成, 左应梅, 杨绍兵, 等. 三七根系分泌物在连作障碍中的生态效应及缓解方法[J]. 中国农业科技导报, 2020, 22(8): 159-167.
	LI H C, ZUO Y M, YANG S B, et al.. Ecological effect of root exudates of panax notoginseng on continuous cropping obstacles and its alleviating methods [J]. J. Agric. Sci. Technol., 2020, 22(8): 159-167.
25	付佳, 王洋, 阎秀峰. 萜类化合物的生理生态功能及经济价值[J]. 东北林业大学学报, 2003, 31(6):59-62.
	FU J, WANG Y, YAN X F. The eco-physiological function and economic value of terpenoids [J]. J. Northeast For. Univ., 2003, 31(6): 59-62.
26	王朋, 王莹, 孔垂华. 植物挥发性单萜经土壤载体的化感作用——以三裂叶豚草(Ambrosia trifida L.)为例[J]. 生态学报, 2008(1): 62-68.
	WANG P, WANG Y, KONG C H. Allelopathy of plant volatile monoterpenes mediated by soil: a case study of Ambrosia trifida L. [J]. Acta Ecol. Sin., 2008(1): 62-68.
27	张一鸣. 人参根际土壤提取物对人参病原菌和拮抗菌的影响研究[D]. 长春:吉林农业大学, 2014.
	ZHANG Y M. Effect of ginseng rhizosphere soil extracts on pathogenic fungi and their antagonistic microorganisms [D]. Changchun: Jilin Agricultural University, 2014.
28	张爱华, 匙坤, 许永华, 等. 立枯丝核菌和菌核菌对人参总皂苷化学趋向性的响应[J]. 西北农林科技大学学报(自然科学版), 2016, 44(5): 200-204.
	ZHANG A H, CHI K, XU Y H, et al.. Chemotaxis response of Rhizoctonia solani and Sclerotinia schinsengto total ginsenosides [J]. J. Northwest Sci-Tech Univ. Agric. For.(Nat. Sci.), 2016, 44(5): 200-204.
29	ZANARDO D, LIMA R B, FERRARESE M, et al.. Soybean root growth inhibition and lignification induced by p-coumaric acid [J]. Environ. Exp. Bot., 2009, 66(1): 25-30.
30	向维. 三七根系分泌物的自毒作用及自毒物质研究[D]. 南宁:广西大学, 2016.
	XIANG W. Autotoxicity in Panax Notoginseng of root exudates [D]. Nanning: Guangxi University, 2016.
31	李培栋,王兴祥,李奕林,等.连作花生土壤中酚酸类物质的检测及其对花生的化感作用[J].生态学报,2010,30(8):2128-2134.
	LI P D, WANG X X, LI Y L, et al.. The contents of phenolic acids in continuous cropping peanut and their allelopathy [J]. Acta Ecol. Sin., 2010,30(8):2128-2134.
32	阎凤鸣. 化学生态学[M].北京: 科学出版社, 2003:1-438.
33	杨靖春, 李治平, 酒井斐子. 人参根系分泌物及其对人参根际微生物作用的研究[J]. 东北师大学报(自然科学版),1982(1): 71-77.
	YANG J C, LI Z P, FEIZI S K. Studies on Ginseng root exudates and their effects on microorganisms in Ginseng rhizosphere [J]. J. Northeast Normal Univ.(Nat. Sci.), 1982(1): 71-77.
34	赵雪淞, 刘民, 马丹丹, 等. 皂苷与植物病原真菌相互关系研究进展[J]. 中国生物防治学报, 2011, 27(3): 404-409.
	ZHAO X S, LIU M, MA D D, et al.. Research advances in interactions between Saponins and Phytopathogenic Fungi [J]. Chin. J. Biol. Control,2011, 27(3): 404-409.

病原菌 Pathogenic bacteria	处理 Treatment/ （mg·L^-1）	抑制率±RSD Inhibiting ratio±RSD/%
病原菌 Pathogenic bacteria	处理 Treatment/ （mg·L^-1）	苯甲酸 Benzoic acid	丁二酸 Succinic acid	己二酸 Adipic acid	邻苯二甲酸 Phthalic acid
强壮土赤壳菌 Ilyonectria robusta	0.001	16.52±4.88^*	-2.36±5.33	5.31±2.29^*	30.09±3.15^*
	0.010	1.06±6.51	10.62±0.72^*	4.87±2.23^*	14.38±1.96^*
	0.100	1.55±6.67	9.73±2.66^*	1.42±1.14	11.50±4.85^*
	1.000	-6.42±6.19	7.96±0.04^*	9.38±4.54^**	13.05±2.33^*
	5.000	20.53±4.65^*	-3.10±4.59	29.87±4.05^*	1.55±9.12
	CK	0.00±2.34	0.00±2.34	0.00±2.34	0.00±2.34
人参核盘菌 Sclerotinia ginseng	0.001	-5.52±4.07	-9.99±3.25^*	-17.25±1.60^*	-0.27±6.67
	0.010	-7.80±4.24^*	-19.70±2.27^*	-17.34±1.79^*	-6.05±2.78
	0.100	-13.05±6.75^*	-21.80±2.27^*	5.85±8.49	-8.15±2.27
	1.000	-14.80±1.21^*	-15.50±1.05^*	2.00±0.61	-10.77±0.61^*
	5.000	-0.27±3.88	3.14±2.33	-19.49±3.69^*	-2.20±2.19
	CK	0.00±2.16	0.00±2.16	0.00±2.16	0.00±2.16
腐皮镰孢菌 Fusarum solani	0.001	-5.60±2.68	-4.33±2.45	-9.41±3.85^*	-3.82±7.78
	0.010	-8.65±2.89^*	-4.58±0.76	-5.85±1.17^*	7.06±2.89
	0.100	-4.58±3.33	-3.31±3.61	-1.02±6.17	7.12±1.17
	1.000	-0.25±4.84	-2.29±0.02	-3.56±1.59	5.85±5.36
	5.000	-1.02±3.09	-2.29±11.90	0.76±0.77	2.80±4.96
	CK	0.00±1.53	0.00±1.53	0.00±1.53	0.00±1.53
人参链格孢菌 Alternaria panax	0.001	-5.42±5.87	-4.58±2.64	9.44±5.96^*	1.04±1.79
	0.010	-2.48±3.68	-3.11±6.75	5.08±0.61	0.95±0.61
	0.100	-8.15±1.82^*	-2.69±0.11	4.19±3.98	-1.50±2.97
	1.000	5.87±1.21^*	0.88±3.20	0.46±4.48	-2.55±0.74
	5.000	10.54±6.19^*	0.88±3.53	-1.64±4.29	-1.59±1.01
	CK	0.00±1.99	0.00±1.99	0.00±1.99	0.00±1.99

病原菌 Pathogenic bacteria	处理 Treatment/ （mg·L^-1）	抑制率±RSD Inhibiting ratio±RSD/%
病原菌 Pathogenic bacteria	处理 Treatment/ （mg·L^-1）	苯甲酸 Benzoic acid	丁二酸 Succinic acid	己二酸 Adipic acid	邻苯二甲酸 Phthalic acid
强壮土赤壳菌 Ilyonectria robusta	0.001	16.52±4.88^*	-2.36±5.33	5.31±2.29^*	30.09±3.15^*
	0.010	1.06±6.51	10.62±0.72^*	4.87±2.23^*	14.38±1.96^*
	0.100	1.55±6.67	9.73±2.66^*	1.42±1.14	11.50±4.85^*
	1.000	-6.42±6.19	7.96±0.04^*	9.38±4.54^**	13.05±2.33^*
	5.000	20.53±4.65^*	-3.10±4.59	29.87±4.05^*	1.55±9.12
	CK	0.00±2.34	0.00±2.34	0.00±2.34	0.00±2.34
人参核盘菌 Sclerotinia ginseng	0.001	-5.52±4.07	-9.99±3.25^*	-17.25±1.60^*	-0.27±6.67
	0.010	-7.80±4.24^*	-19.70±2.27^*	-17.34±1.79^*	-6.05±2.78
	0.100	-13.05±6.75^*	-21.80±2.27^*	5.85±8.49	-8.15±2.27
	1.000	-14.80±1.21^*	-15.50±1.05^*	2.00±0.61	-10.77±0.61^*
	5.000	-0.27±3.88	3.14±2.33	-19.49±3.69^*	-2.20±2.19
	CK	0.00±2.16	0.00±2.16	0.00±2.16	0.00±2.16
腐皮镰孢菌 Fusarum solani	0.001	-5.60±2.68	-4.33±2.45	-9.41±3.85^*	-3.82±7.78
	0.010	-8.65±2.89^*	-4.58±0.76	-5.85±1.17^*	7.06±2.89
	0.100	-4.58±3.33	-3.31±3.61	-1.02±6.17	7.12±1.17
	1.000	-0.25±4.84	-2.29±0.02	-3.56±1.59	5.85±5.36
	5.000	-1.02±3.09	-2.29±11.90	0.76±0.77	2.80±4.96
	CK	0.00±1.53	0.00±1.53	0.00±1.53	0.00±1.53
人参链格孢菌 Alternaria panax	0.001	-5.42±5.87	-4.58±2.64	9.44±5.96^*	1.04±1.79
	0.010	-2.48±3.68	-3.11±6.75	5.08±0.61	0.95±0.61
	0.100	-8.15±1.82^*	-2.69±0.11	4.19±3.98	-1.50±2.97
	1.000	5.87±1.21^*	0.88±3.20	0.46±4.48	-2.55±0.74
	5.000	10.54±6.19^*	0.88±3.53	-1.64±4.29	-1.59±1.01
	CK	0.00±1.99	0.00±1.99	0.00±1.99	0.00±1.99

病原菌 Pathogenic bacteria	处理 Treatment/（mg·L^-1）	抑制率±RSD Inhibiting ratio±RSD/%
病原菌 Pathogenic bacteria	处理 Treatment/（mg·L^-1）	Rb2	Rc	Rd	Re	Rg1
强壮土赤壳菌 Ilyonectria robusta	0.001	0.00±4.45	19.12±4.83^*	14.82±6.15^*	44.07±3.03^*	6.64±2.34
	0.010	12.39±5.87^*	30.09±5.04^*	19.47±1.53^*	18.23±3.52^*	-2.95±56.06
	0.100	27.08±2.91^*	7.30±3.91^*	30.80±3.42^*	15.75±0.85^*	8.85±4.40^*
	1.000	24.78±4.68^*	7.96±4.57^*	0.00±3.38	14.16±1.77^*	31.33±8.46^*
	5.000	13.86±4.09^*	-1.77±5.45	-16.81±2.17^*	23.89±5.06^*	17.70±3.15^*
	CK	0.00±2.34	0.00±2.34	0.00±2.34	0.00±2.34	0.00±2.34
人参核盘菌 Sclerotinia ginseng	0.001	-1.32±6.09	-5.26±6.61	-7.07±2.92	-2.90±3.74	-22.85±2.78^*
	0.010	-6.05±1.58^*	-3.73±8.55	-8.50±7.60	-12.09±1.99^*	13.64±0.53^*
	0.100	-9.55±0.61^*	-17.60±4.77^*	-8.67±6.09	-18.91±4.95^*	-15.85±0.61^*
	1.000	-3.95±3.09	-11.82±5.18^*	-16.20±1.21^*	-2.71±2.41	-20.75±1.21^*
	5.000	7.86±1.60^*	-22.59±1.32^*	-14.80±7.60^*	-3.42±6.67	-6.31±1.01
	CK	0.00±2.16	0.00±2.16	0.00±2.16	0.00±2.16	0.00±2.16
腐皮镰孢菌 Fusarum solani	0.001	1.78±6.49	-1.08±3.08	8.14±7.53	-7.12±4.91	-4.35±1.92
	0.010	-5.85±5.78	-8.65±2.89	4.83±3.92	-0.51±2.2	-7.38±1.92^*
	0.100	-4.83±5.36	-3.82±2.02	-3.56±1.17	0.25±0.88	3.56±3.08
	1.000	-8.91±1.17^*	-1.02±1.17	1.02±4.20	-1.27±3.18	6.62±7.33
	5.000	-5.85±0.88	-3.82±2.75	2.54±5.83	5.09±7.72	0.95±1.59
	CK	0.00±1.53	0.00±1.53	0.00±1.53	0.00±1.53	0.00±1.53
人参链格孢菌 Alternaria panax	0.001	-2.06±2.72	-2.55±3.38	-9.83±3.61^*	-0.54±4.06	-8.41±2.33^*
	0.010	-7.73±2.05^*	-3.11±3.67	-2.48±0.33	-1.85±6.47	-1.64±1.73
	0.100	-3.32±7.57	2.14±1.05	-2.27±2.42	-1.59±1.60	-3.74±4.37
	1.000	-1.75±2.72	-6.47±1.60^*	5.50±3.38	1.30±3.98	0.04±2.78
	5.000	-3.32±6.87	-3.95±3.71^*	2.00±3.21	2.00±5.78	2.00±4.24
	CK	0.00±1.99	0.00±1.99	0.00±1.99	0.00±1.99	0.00±1.99

病原菌 Pathogenic bacteria	处理 Treatment/（mg·L^-1）	抑制率±RSD Inhibiting ratio±RSD/%
病原菌 Pathogenic bacteria	处理 Treatment/（mg·L^-1）	Rb2	Rc	Rd	Re	Rg1
强壮土赤壳菌 Ilyonectria robusta	0.001	0.00±4.45	19.12±4.83^*	14.82±6.15^*	44.07±3.03^*	6.64±2.34
	0.010	12.39±5.87^*	30.09±5.04^*	19.47±1.53^*	18.23±3.52^*	-2.95±56.06
	0.100	27.08±2.91^*	7.30±3.91^*	30.80±3.42^*	15.75±0.85^*	8.85±4.40^*
	1.000	24.78±4.68^*	7.96±4.57^*	0.00±3.38	14.16±1.77^*	31.33±8.46^*
	5.000	13.86±4.09^*	-1.77±5.45	-16.81±2.17^*	23.89±5.06^*	17.70±3.15^*
	CK	0.00±2.34	0.00±2.34	0.00±2.34	0.00±2.34	0.00±2.34
人参核盘菌 Sclerotinia ginseng	0.001	-1.32±6.09	-5.26±6.61	-7.07±2.92	-2.90±3.74	-22.85±2.78^*
	0.010	-6.05±1.58^*	-3.73±8.55	-8.50±7.60	-12.09±1.99^*	13.64±0.53^*
	0.100	-9.55±0.61^*	-17.60±4.77^*	-8.67±6.09	-18.91±4.95^*	-15.85±0.61^*
	1.000	-3.95±3.09	-11.82±5.18^*	-16.20±1.21^*	-2.71±2.41	-20.75±1.21^*
	5.000	7.86±1.60^*	-22.59±1.32^*	-14.80±7.60^*	-3.42±6.67	-6.31±1.01
	CK	0.00±2.16	0.00±2.16	0.00±2.16	0.00±2.16	0.00±2.16
腐皮镰孢菌 Fusarum solani	0.001	1.78±6.49	-1.08±3.08	8.14±7.53	-7.12±4.91	-4.35±1.92
	0.010	-5.85±5.78	-8.65±2.89	4.83±3.92	-0.51±2.2	-7.38±1.92^*
	0.100	-4.83±5.36	-3.82±2.02	-3.56±1.17	0.25±0.88	3.56±3.08
	1.000	-8.91±1.17^*	-1.02±1.17	1.02±4.20	-1.27±3.18	6.62±7.33
	5.000	-5.85±0.88	-3.82±2.75	2.54±5.83	5.09±7.72	0.95±1.59
	CK	0.00±1.53	0.00±1.53	0.00±1.53	0.00±1.53	0.00±1.53
人参链格孢菌 Alternaria panax	0.001	-2.06±2.72	-2.55±3.38	-9.83±3.61^*	-0.54±4.06	-8.41±2.33^*
	0.010	-7.73±2.05^*	-3.11±3.67	-2.48±0.33	-1.85±6.47	-1.64±1.73
	0.100	-3.32±7.57	2.14±1.05	-2.27±2.42	-1.59±1.60	-3.74±4.37
	1.000	-1.75±2.72	-6.47±1.60^*	5.50±3.38	1.30±3.98	0.04±2.78
	5.000	-3.32±6.87	-3.95±3.71^*	2.00±3.21	2.00±5.78	2.00±4.24
	CK	0.00±1.99	0.00±1.99	0.00±1.99	0.00±1.99	0.00±1.99

[1]	张娜娜§, 李双民§, 温晓蕾, 冯丽娜, 王俊凤, 杨文杰, 霍佳欢, 兰淑慧, 孙伟明, 齐慧霞. 板栗红粉病病原菌鉴定及其生物学特性研究[J]. 中国农业科技导报, 2021, 23(7): 145-152.
[2]	李浩成1，2，左应梅2，杨绍兵2，杨天梅2，李纪潮2，杨维泽2，张金渝2*. 三七根系分泌物在连作障碍中的生态效应及缓解方法[J]. 中国农业科技导报, 2020, 22(8): 159-167.
[3]	温晓蕾1,2,齐慧霞1*,孙伟明1,刘一健1,冯丽娜1,孟童瑶3,韩志玲1,曹佳1,王俊凤1. 北苍术枝枯病病原菌（Fusarium equiseti）的鉴定及其生物学特性研究[J]. 中国农业科技导报, 2020, 22(5): 115-121.
[4]	李庆凯1,2,3,刘苹2,3,赵海军3,宋效宗2,林海涛2,沈玉文2,李林1,万书波1,3. 玉米根系分泌物对连作花生土壤酚酸类物质化感作用的影响[J]. 中国农业科技导报, 2020, 22(3): 119-130.
[5]	王永杰,陈红莲,程云生,王芬. 黄金鲫内脏类结节病的病原鉴定与药物敏感性试验分析[J]. 中国农业科技导报, 2017, 19(12): 104-109.
[6]	张翠绵1,李洪涛1,李晓芝2,贾楠1,胡栋1,王占武1. 链霉菌S506对设施黄瓜根际生态和生产性状的影响[J]. , 2010, 12(5): 98-102.
[7]	倪志华,李晶,廖娇,徐小彪. 果树根际微生态研究现状及展望[J]. , 2010, 12(4): 34-38.
[8]	张子龙,王文全. 药用植物连作障碍的形成机理及其防治[J]. , 2009, 11(6): 19-23.
[9]	薛泉宏,同延安. 土壤生物退化及其修复技术研究进展[J]. , 2008, 10(4): 28-35.
[10]	李花粉. 根际重金属污染[J]. , 2000, 2(4): 54-59.
[11]	张福锁,申建波. 根际微生态系统理论框架的初步构建[J]. , 1999, 1(4): 15-20.
[12]	刘永秀,张福锁,毛达如. 根际微生态系统中豆科植物-根瘤菌共生固氮及其在可持续农业发展中的作用[J]. , 1999, 1(4): 28-33.
[13]	李隆,左元梅,刘永秀,李晓林,张福锁. 根际微生态系统理论在我国农业高产高效中的作用——间套种作物种间的根际微生态效应的发现及应用前景[J]. , 1999, 1(4): 34-40.
[14]	阮维斌,王敬国,张福锁,申建波. 根际微生态系统理论在连作障碍中的应用[J]. , 1999, 1(4): 53-58.
[15]	段玉琪1,陈冬梅2,晋艳1,王海斌2,杨宇虹1,尤垂淮2,田卫霞2,林文雄2. 不同肥料对连作烟草根际土壤微生物及酶活性的影响[J]. , 1, 1(1): 122-126.