关键词: 植物;多基因转化;农杆菌;基因枪

Abstract:

With the rapid development of genetic engineering and molecular biology, the plant genetic improvement means are innovated continuously. For transgenetic plant, single gene transformation technology is insufficient to meet the needs of plant modifications, especially for some genes related to metabolism pathways and quantity traits. So multi-gene transformation comes into being and develops quickly. This paper reviewed the progress made on multi-gene transformation mediated with Agrobacterium and particle bombardment with tobacco, rice, and corn as examples. Problems and prospects of those techniques were also discussed to provide references to multi-gene transformation of main crops such as wheat etc. and to promote transgenic breeding.

Key words: plant, multi-gene transformation, Agrobacterium tumefaciens, particle bombardment