›› 2008, Vol. 10 ›› Issue (3): 31-39.

• 综述 • 上一篇    下一篇

商品化转基因植物的外源基因及其检测技术

李俊1,郑秀丽2,邓平建3,刘国振1,2   

  1. (1.河北农业大学生命科学学院, 河北 保定 071000; 2.中国科学院北京基因组研究所, 北京 101300, 3.深圳市疾病预防控制中心, |深圳 518020)
  • 收稿日期:2008-03-03 修回日期:2008-05-14 出版日期:2008-06-15 发布日期:2009-07-29
  • 通讯作者: 刘国振,教授,主要从事植物分子生物学研究。Tel: 010-80485460; E-mail: gzhliu@genomics.org.cn
  • 作者简介:李俊,硕士研究生,主要从事转基因植物检测芯片的制备及其应用技术研究。
  • 基金资助:

    国家973项目(2007CB109201)资助。

Heterogenic Genes for Commercialized Genetically Modified
Plants and Their Detection Techniques


LI Jun1, ZHENG Xiu-li2, DENG Ping-jian3, LIU Guo-zhen1,2   

  1. (1.College of Life Sciences, Agricultural University of Hebei, Baoding 071000|2.Beijing Institute of Genomics, Chinese Academy
    of Sciences, Beijing 101300|3. Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China)
  • Received:2008-03-03 Revised:2008-05-14 Online:2008-06-15 Published:2009-07-29

摘要:

随着多种生物基因组的测序、分子生物学及转基因技术的发展,被批准大田释放的转基因植物越来越多。本文总结了转基因植物涉及的外源基因

,包括辅助转化的基因、改良植物性状的目的基因及调控目的基因表达的序列等3大类44个基因;列举了美国和欧盟批准大田释放的转基因植物

及品系;我国农业部批准的可进口用作加工原料的转基因植物。对转基因植物及加工产品的定性、定量检测技术进行了介绍,包括普通、定量及

多重PCR技术、多重PCR结合芯片或肽核酸检测技术,以及基于表达蛋白质的检测技术等,最后对转基因植物及检测技术的发展进行了总结和展望

关键词: 转基因植物;外源基因;遗传标记;PCR

Abstract:

With the development of genome sequencing, molecular biology and transgenic techniques, the number of transgenic plants

authorized for release was increased rapidly. Genetic elements involved in transgenic plants, including selectable markers,

target genes and regulatory genes belongs to three categories (44 genes in total) were summarized in this paper. Plant

species and transgenic events authorized for release by US and European Union, genetically modified materials approved for

import by the Ministry of Agriculture of China were also summarized. The progress for the qualitative and quantitative

detection techniques, including PCR, microarray and combined techniques were discussed in the paper. The development of

transgenic plants and their detection technigues were summarized and outlooked at the end.

Key words: genetic modified organisms, foreign DNA, genetic marker, PCR

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