关键词: 海带废渣;真姬菇;抗氧化;羟自由基;超氧阴离子自由基

Abstract:

In order to develop a new pathway to utilize kelp waste, Hypsizigus marmoreus was incubated in medium mainly composed of kelp waste and products from fermentation were investigated with antioxidant assays. The results showed that mushroom could grow in this medium and produce abundant mycelia. Antioxidant assays indicated that the total antioxidant capacity (T-AOC) of the supernatant and extract of mycelia were 0.23±0.02 U/mg and 0.77±0.01 U/mg, which were 43.8% and 18.5% higher than those of the medium; and also significantly higher than that of the chemical antioxidant BHT. Furthermore, the supernatant of fermentation broth exhibited potent capacity for hydroxyl radical scavenging and superoxide anion radical scavenging, as their EC50 values were 0.43 mg/mL and 26.02 mg/mL. The study showed that the compounds with potent antioxidant activity produced during the fermentation mainly distribute inside of the mycelia, and the compounds with potent capacity for hydroxyl radical scavenging mainly distribute in the supernatant.

Key words: kelp waste, Hypsizigus marmoreus, antioxidant, hydroxyl radical, superoxide anion radical