枯草芽胞杆菌芽胞表面展示外源蛋白的研究进展

doi:doi:10.3969/j.issn.10080864.2013.05.05

›› 2013, Vol. 15 ›› Issue (5): 31-38.DOI: doi:10.3969/j.issn.10080864.2013.05.05

• 农业微生物制剂与酶产品创制特稿 • 上一篇下一篇

枯草芽胞杆菌芽胞表面展示外源蛋白的研究进展

余小霞,田健,伍宁丰*

（中国农业科学院生物技术研究所, 北京 100081）

出版日期:2013-10-15 发布日期:2013-12-16
通讯作者: 伍宁丰，研究员，主要从事微生物分子生物学与基因工程研究。E\|mail：wunf@caas.net.cn
作者简介:余小霞|硕士研究生|研究方向微生物分子生物学与基因工程。E\|mail：yuxiaoxia198921@sina.com。
基金资助:
国家863计划项目（2013AA102804）资助。

Research Progress on Bacillus subtilis Spore Display of Recombinant Proteins

YU Xiao\|xia, TIAN Jian, WU Ning\|feng*

(Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Online:2013-10-15 Published:2013-12-16

摘要/Abstract

摘要：

枯草芽胞杆菌是一种生物安全的革兰氏阳性细菌，在营养匮乏的环境下，可形成具有强抗逆性的芽胞。枯草芽胞杆菌的芽胞由核心、皮层、孢子外套蛋白三部分组成，目前已成功利用枯草芽胞杆菌芽胞外套蛋白CotB、CotC、CotG、CotX和OxdD为载体，将酶蛋白、抗原蛋白或荧光标记蛋白等展示于芽胞表面。芽胞表面展示的蛋白通常具有较好的稳定性、易于纯化和安全性好等优点，可应用于医药、食品及饲料工业等领域，具有较大的应用前景。详细介绍了枯草芽胞杆菌芽胞的分子特点及芽胞表面展示系统的构建过程及其应用前景，为芽胞表面展示载体的基础及应用研究奠定基础。

关键词: 枯草芽胞杆菌;芽胞;外源蛋白;芽胞表面展示

Abstract:

Bacillus subtilis is Gram\|positive bacteria with biological safy. It can form spores with strong stress resistance in poor nutrient environment. The spore of Bacillus subtilis consists of 3 parts including the core, cortex and spore coat protein. Recently, the Bacillus subtilis spore coat proteins, such as CotB, CotC, CotG, CotX and OxdD, have been successfully used as vectors to display the antigen proteins, enzymes or reporter protein on the spore surfaces. The recombinant proteins on Bacillus subtilis spores usually have many advantages, such as good stability, easy purification and safety. Therefore, they can be used in medicine, food and feed industry, and other fields. It has a great application prospect. This review introduced in detail the molecular characteristics of Bacillus subtilis spores, the construction process of the expressive system on spore surface and its application prospect. Thus, the paper provided a foundation for the basic and applied research about spore surface display system.

Key words: Bacillus subtilis, spore, recombinant proteins, spore surface display

中图分类号:

Q939.9

余小霞,田健,伍宁丰*. 枯草芽胞杆菌芽胞表面展示外源蛋白的研究进展[J]. , 2013, 15(5): 31-38.

YU Xiao\|xia, TIAN Jian, WU Ning\|feng*. Research Progress on Bacillus subtilis Spore Display of Recombinant Proteins[J]. , 2013, 15(5): 31-38.

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枯草芽胞杆菌芽胞表面展示外源蛋白的研究进展

Research Progress on Bacillus subtilis Spore Display of Recombinant Proteins

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