鸡油菌转化虾副产品产血管紧张素转换酶抑制剂的条件优化

doi:10.13304/j.nykjdb.2015.295

中国农业科技导报 ›› 2016, Vol. 18 ›› Issue (1): 171-181.DOI: 10.13304/j.nykjdb.2015.295

鸡油菌转化虾副产品产血管紧张素转换酶抑制剂的条件优化

高秀君,闫培生*

(哈尔滨工业大学（威海）海洋科学与技术学院, 山东威海 264209)

收稿日期:2015-05-25 修回日期:2015-08-28 出版日期:2016-02-15 发布日期:2016-02-15
通讯作者: 闫培生，教授，博士生导师，研究方向为海洋微生物应用研究及水产加工废弃物资源化研究。E-mail:yps6@163.com
作者简介:高秀君|讲师|硕士|研究方向为工农业废弃物的资源化。E-mail:gaoxiujun@hitwh.edu.cn。

Condition Optimization of Bioconversion Shrimp Processing Wastes with Cantharellus cibarius for Angiotensin I-Converting Enzyme (ACE) Inhibitors

GAO Xiu-jun, YAN Pei-sheng*

(School of Marine Science and Technology, Harbin Institute of Technology (Weihai), Shandong Weihai 264209, China)

Received:2015-05-25 Revised:2015-08-28 Online:2016-02-15 Published:2016-02-15

摘要/Abstract

摘要：

为探索高附加值回收利用虾加工废弃物的方法，利用鸡油菌（Cantharellus cibarius）转化虾加工废弃物，以PB设计、最陡爬坡试验和响应面设计对转化条件进行优化，以血管紧张素转换酶（ACE）抑制活性为指标考察了转化产物的抗高血压活性。结果表明，鸡油菌转化虾加工废弃物最优液体发酵条件（兼顾菌丝产量和ACE抑制活性）为料液比1∶1.857，冰醋酸加入量1.25%；对该转化条件的验证实验结果与发酵条件数学模型预测值无显著性差异（P>0.05），在此条件下菌丝体产量为（27.837±1.011） g/L，较优化前提高21.231%；菌丝体水提物ACE抑制IC50为（0.394±0.071） mg/mL，显著低于优化前（P<0.05），脱腥得分为3.611±0.044。以药用价值和经济价值较高的鸡油菌转化虾加工废弃物，其产物具有较高的ACE抑制活性，可从其中分离得到较高活性的ACE抑制剂，而且可使产物脱腥。

关键词: 虾加工废弃物, 生物转化, 鸡油菌, ACE抑制剂（ACEI）

Abstract:

To explore the methods for recycling shrimp processing wastes with high added value, shrimp processing wastes were bio-converted by edible/medicinal fungus Cantharellus cibarius through submerged fermentation in this study. And then, the conditions of bioconversion were optimized through Placket-Burman (PB) experimental design, Path of Steepest Ascent and Response Surface Methodology (RSM) in sequence. Antihypertensive activity of the water extracted from converted products was determined through testing the inhibitory activity of the water extracts on Angiotensin I-Converting Enzyme (ACE). The results indicated that the optimized bioconversion model of Cantharellus cibariuss was established as 1∶1.857 of solid to liquid radio and 1.25% of concentration of glacial acetic acid, when the mycelium biomass and the ACE inhibitory activity of water extracts from mycelium were both kept in mind. Under this model, the mycelium biomass reached 27.837 g/L, improved by 21.231%. ACE inhibitory activity (IC50=0.394±0.071 mg/mL) of water extracts from mycelium was decreased significantly (P<0.05). The score of deodorization was calculated as 3.611±0.044. Bioconversion shrimp processing wastes by Cantharellus cibariuss had the effect of deodorization, and ACEI with relatively higher activity could be isolated from the bio-converted products.

Key words: shrimp processing wastes, bioconversion, Cantharellus cibarius, angiotensin I-converting enzyme inhibitor (ACEI)

中图分类号:

X714

高秀君,闫培生*. 鸡油菌转化虾副产品产血管紧张素转换酶抑制剂的条件优化[J]. 中国农业科技导报, 2016, 18(1): 171-181.

GAO Xiu-jun, YAN Pei-sheng*. Condition Optimization of Bioconversion Shrimp Processing Wastes with Cantharellus cibarius for Angiotensin I-Converting Enzyme (ACE) Inhibitors[J]. Journal of Agricultural Science and Technology, 2016, 18(1): 171-181.

[1]	蔡小雨1,闫培生1*,高秀君1,陈琪琪1,郭长禄1,刘润东2,梁浩2,张明臣2. 人参皂苷生物转化的研究进展[J]. 中国农业科技导报, 2018, 20(4): 52-60.
[2]	刘元利1,陈吉祥1*,王永刚2,李彦林1,程琳1,周永涛3. 一株产热稳定性脂肪酶莱茵海默氏菌（Rheinheimera sp.）NT-1的分离及其酶学性质分析[J]. 中国农业科技导报, 2016, 18(6): 80-89.
[3]	高翠娟. 定向突变谷氨酸脱羧酶及其生物合成γ-氨基丁酸的研究[J]. 中国农业科技导报, 2016, 18(2): 59-64.
[4]	彭亮亮1,2,王自强2,王云山2,张利平1,苏志国2. 顺式环氧琥珀酸水解酶酶活的快速测定方法[J]. , 2010, 12(1): 135-138.

鸡油菌转化虾副产品产血管紧张素转换酶抑制剂的条件优化

Condition Optimization of Bioconversion Shrimp Processing Wastes with Cantharellus cibarius for Angiotensin I-Converting Enzyme (ACE) Inhibitors

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics