关键词: 黄曲霉毒素, 抑毒活性成分, 花生

Abstract: In order to develop biological preservatives that can effectively improve the resistant ability of peanut and other agricultural products to aflatoxin contamination, an isolated biocontrol strain was identified with 16S rDNA and its anti-mold and anti-aflatoxin characteristics were studied. The results showed that it was a Bacillus subtilis, the ability to produce anti-aflatoxigenic effective components was not affected by subculture quantity. The fungi strain could produce antia-flatoxigenic effective components in wide fermentation temperature range. The initial pH value of the optimal culture medium for the strain was 6, and the optimal culture time for the generation of anti-aflatoxigenic effective components was 6 d. The yield of anti-aflatoxigenic effective components extracted by methanol from strain fermentation supernatant was the highest and the activity was the strongest. The mold infection of peanuts indicated that the inhibition rate for norsolorinic acid (NA) accumulation reached 84% or more when 0.2～3.2 mg/mL anti-aflatoxigenic effective components were added, which was higher than that of sodium diacetate (SDA) used. The anti-aflatoxigenic effective components palyed  good roles in the anti-mildew and anti-virus treatment of peanut. The results showed that the anti-aflatoxigenic effective components exacted from B. subtilis Hitwh-B05  improved anti-mildew and anti-virus abilities of agricultural products, and prevented the mildew and virus infection from the source. Its use can decrease the harm of chemical preservatives to environment and human.

Key words: aflatoxin, antiaflatoxigenic effective components, peanut