免疫亲和净化-光化学衍生液相色谱检测不同样品中的黄曲霉毒素

doi:10.13304/j.nykjdb.2019.0393

中国农业科技导报 ›› 2020, Vol. 22 ›› Issue (7): 181-187.DOI: 10.13304/j.nykjdb.2019.0393

• 方法与技术创新 • 上一篇

免疫亲和净化-光化学衍生液相色谱检测不同样品中的黄曲霉毒素

华丽霞,曾华兰,蒋秋平,何炼,叶鹏盛*,张小军,韦树谷,张小红,张敏,王明娟,何晓敏,陈超

四川省农业科学院经济作物育种栽培研究所, 成都 610300

收稿日期:2019-05-13 出版日期:2020-07-15 发布日期:2019-06-10
通讯作者: *通信作者叶鹏盛 E-mail: yeps18@163.com
作者简介:华丽霞 E-mail: newpage@stu.scau.edu.cn;
基金资助:
四川省应用基础研究项目（2017JY0288）；四川省重点研发项目（2018NZ0011）；四川省财政创新能力提升工程青年基金项目（2018QNJJ-002）。

Detection of Aflatoxin in Different Samples by Immunoaffinity Purification Combined with Photochemical Derivatization High-performance Liquid Chromatography

HUA Lixia, ZENG Hualan, JIANG Qiuping, HE Lian, YE Pengsheng*, ZHANG Xiaojun, WEI Shugu, ZHANG Xiaohong, ZHANG Min, WANG Mingjuan, HE Xiaomin, CHEN Chao

Industrial Crops Breeding and Cultivation Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610300, China

Received:2019-05-13 Online:2020-07-15 Published:2019-06-10

摘要/Abstract

摘要： 黄曲霉毒素（aflatoxin，AFT）是一类具有高毒性的真菌毒素，常见的有AFTB1、AFTB2、AFTG1、AFTG2。对黄曲霉毒素进行快速、准确的检测是保障食品安全的重要技术手段。利用免疫亲和净化结合光化学柱后衍生液相色谱的方法对油脂类、坚果类及黄曲霉菌株培养物3种类型样品中的AFTB1、AFTB2、AFTG1及AFTG2进行定性定量分析，探索该方法对不同类型样品的净化及检测效果。结果表明：免疫亲和柱对3种类型样品的净化效果良好，能特异性对黄曲霉毒素进行吸附，没有明显的干扰峰。经过光化学衍生反应装置衍生后，4种黄曲霉毒素在25 min内得到良好的基线分离，AFTB1、AFTG1在0.1~40.0 ng·mL-1的范围内有良好的线性关系，AFTB2、AFTG2在0.03~12.0 ng·mL-1的范围内有良好的线性关系。利用免疫亲和净化-光化学衍生液相色谱法不仅能准确检测出油脂类及坚果类样品中的黄曲霉毒素，还能用于监测黄曲霉菌株的产毒情况及毒素积累规律，可为黄曲霉毒素的研究与防控提供重要技术支撑。

关键词: 黄曲霉毒素, 免疫亲和, 光化学衍生, 液相色谱, 食品安全

Abstract: Aflatoxin （AFT） is a type of mycotoxin that is considered to be a human carcinogen, which commonly includes AFTB1, AFTB2, AFTG1 and AFTG2. Rapid and accurate detection of AFT is important for food safety security. In this paper, a high-performance liquid chromatography (HPLC) method combined immunoaffinity column (IAC) with post-column derivation by photochemical reactor was carried out for sensitive detection of AFTB1, AFTB2, AFTG1, AFTG2 from 3 types of samples, including oil, nut and Aspergillus flavus cultures. The results showed that: IAC had good purification effect on the 3 types of samples, and could specifically adsorb AFT without obvious interference peak. After photochemical derivatization, AFTB1, AFTB2, AFTG1 and AFTG2 could be separated clearly within 25 min. AFTB1 and AFTG1 had a good linear relationship in the range of 0.1~40.0 ng·mL-1, and AFTB2 and AFTG2 had a good linear relationship in the range of 0.03~12.0 ng·mL-1. The application of immunoaffinity purification combined with photochemical derivatization high-performance liquid chromatography could not only accurately detect AFT in oil and nut samples, but also monitor the toxin production and toxin accumulation of AFT strains, which would provide important technical support for AFT research and prevention and control.

Key words: aflatoxin, immunoaffinity, photochemical derivatization, liquid chromatography, food safety

华丽霞,曾华兰,蒋秋平,何炼,叶鹏盛*,张小军,韦树谷,张小红,张敏,王明娟,何晓敏,陈超. 免疫亲和净化-光化学衍生液相色谱检测不同样品中的黄曲霉毒素[J]. 中国农业科技导报, 2020, 22(7): 181-187.

HUA Lixia, ZENG Hualan, JIANG Qiuping, HE Lian, YE Pengsheng*, ZHANG Xiaojun, WEI Shugu, ZHANG Xiaohong, ZHANG Min, WANG Mingjuan, HE Xiaomin, CHEN Chao. Detection of Aflatoxin in Different Samples by Immunoaffinity Purification Combined with Photochemical Derivatization High-performance Liquid Chromatography[J]. Journal of Agricultural Science and Technology, 2020, 22(7): 181-187.

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免疫亲和净化-光化学衍生液相色谱检测不同样品中的黄曲霉毒素

Detection of Aflatoxin in Different Samples by Immunoaffinity Purification Combined with Photochemical Derivatization High-performance Liquid Chromatography

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