关键词: 拟南芥;电压依赖型阴离子通道;水杨酸;信号传递

Abstract:

Voltage-dependent anion channels (VDAC) proteins distribute in mitochondrial outer membrane. They participate in the formation of mitochondrial permeability transition pore (PTP) and control substances in and out of the mitochondria. To explore the possibility of VDAC in SA signaling pathway, Arabidopsis thaliana (RLD) was taken as experiment material, and transgenic plant lines of VDAC over-expression and inhibited expression were gotten. Then germination experiment of transgenic plant seeds were treated with SA. The result showed that VDAC affected the sensitivity to SA of transgenic seeds. The over-expression lines seeds were sensitive to SA, with low germination rate. In contrast, the inhibited expression lines were less sensitive and the germination rate was high. PR1, marker gene of SA signaling pathway, was used for the real-time fluorescence quantitative PCR. It was found that in over-expression lines, accompanied by VDAC high level, PR1 increased; and in the inhibition lines, VDAC lower level, PR1 declined. In summary, Arabidopsis thaliana VDAC was involved in SA signaling pathway.

Key words: Arabidopsis thaliana, voltage-dependent anion channels(VDAC), SA, signaling pathway