Abstract: The nutrient contents of Auricularia auricula produced at different areas are different. Therefore, it has practical value to identify the producing areas of A. auricula. This paper selected 8 kinds of A. auricula from different producing areas; analyzed them by Fourier transform infrared spectroscopy (FT-IR) two-dimensional correlation infrared (2D-IR) spectroscopy; and identified them with hierarchical cluster analysis (HCA). The results showed that fourier transformed infrared (FT-IR) spectra of 8 samples resembled extremely in holistic spectral profile, and their partial correlation coefficient was no less than 0.955, which could not be used to distinguish their origin. In their 2D-IR spectra, the numbers of auto-peaks were different in the range of 1 730~1 400 cm-1, 1 350~1 040 cm-1 and 1 030~900 cm-1. Meanwhile, there were more differences in the intensities of auto-peaks and cross-peaks. Hierarchical cluster analysis was conducted on A. auricula with FT-IR spectra in the range of 1 700~950 cm-1, and all samples from different areas were distinguished correctly. These results demonstrated that A. auricula produced at different areas could be simply and rapidly identified by 2D-IR combined with hierarchical cluster analysis without prejudice.

Key words: Fourier transformed infrared (FT-IR) spectroscopy, two-dimensional correlation infrared (2D-IR) spectroscopy, hierarchical cluster analysis (HCA), identification, different producing area, Auricularia auricula

摘要： 不同产地黑木耳的营养成分含量存在差异，因此对黑木耳的产地鉴别具有实用价值。选取8个不同产地的黑木耳，采用傅里叶变换红外光谱（Fourier transform infrared spectroscopy，FT-IR）和二维相关红外光谱（two-dimensional correlation infrared spectroscopy，2D-IR)，对不同样本的2D-IR谱图进行处理和分析，并结合系统聚类分析以鉴别黑木耳的不同产地。结果显示，不同产地黑木耳的傅里叶变换红外光谱整体特征极为相似，偏相关系数的最小值为0.955，不能用于区分黑木耳的产地。不同产地黑木耳在1 730~1 400 cm-1、1 350~1 040 cm-1以及1 030~900 cm-1范围内的二维相关红外光谱中自动峰数目不同，它们之间的自动峰和交叉峰的强度也有较大差异；对1 700~950 cm-1范围内的FT-IR光谱进行系统聚类分析，不同产地黑木耳都得到正确归类。结果表明，二维相关红外光谱分析，结合系统聚类分析能够简单、快速、无损地鉴别不同产地黑木耳。

关键词: 傅里叶变换红外光谱, 二维相关红外光谱, 系统聚类分析, 鉴别, 不同产地, 黑木耳