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Table of Content

    15 January 2019, Volume 21 Issue 1
    Application of Big Data in Precision Agriculture
    QI Haixia1,2, LIN Zhenxin1,2, LAN Yubin1,2*
    2019, 21(1):  1-10.  DOI: 10.13304/j.nykjdb.2018.0342
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    Precision agriculture is the future way for agricultural modernization. The rapid expansion of agricultural data and the development of big data technology provide a new method for the development of precision agriculture, and become an important force leading to the development of precision agriculture. Firstly, this paper introduced the definition and acquisition methods of big data. At the same time, the concept of precision agriculture were introduced for some problems existing in the current agricultural field, and its source and development conditions. Combining the industrial chain of agriculture with the application of big data, this paper analyzed the process of big data to achieve precision agriculture, and the problems in the application of Chinas agricultural big data.
    Impact of African Swine Fever on the Development of Pig Industry in China
    NIE Yunbin, QIAO Juan*
    2019, 21(1):  11-17.  DOI: 10.13304/j.nykjdb.2018.0601
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    African swine fever has occurred in many provinces or regions in China, which has a great impact on the development of pig industry. This paper summarized the occurrence of African swine fever, elaborated the prevention and control measures adopted by China since the occurrence of African swine fever and the existing difficulties in prevention and control, and then analyzed the impact of African swine fever on the pig industry in the affected areas and the pork market in China. In order to prevent and control African swine fever and other similar major epidemics, it was necessary to establish and improve the monitoring and prevention system and pork traceability system, improve consumer awareness, protect the interests of farmers, focus on the integration and extension of the industrial chain, and strengthen the construction of grassroots teams etc. coutermeasures.
    Research Advances of Plant Tissue Specific Promoters
    JIAO Yong1,2, LIU Xiaoqing2, JIANG Haiyang1*, CHEN Rumei2*
    2019, 21(1):  18-28.  DOI: 10.13304/j.nykjdb.2018.0088
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    During the different development stages and interaction with environment, plant structure and morphology will change dramatically. These changes are controlled by a set of genes expressed in specific spatial and temporal profile. The precise “switch on” is plant tissue specific promoter. Due to the tissue specificity of these promoters, there are currently a great deal of researches on model plants (Arabidopsis thaliana, Nicotiana tabacum), food crops (Zea mays, Oryza sativa, Triticum aestivum, etc.) and economic crops (Anemone vitifolia Buch, Brassica napus, Arachis hypogaea, etc.). This paper reviewed the advances of plant tissue specific promoters and prospected innovative approaches for isolation and application of tissue specific promoters.
    Dynamic Analysis of Enzyme Activities and Expression of Genes Related to Sugar Metabolism During the Development of Tobacco
    LI Zheng1, PAN Feilong1, FANG Ming2, TAN Fangli2, WU Wenxin2, LIU Xiaobin2, LI Hongguang2, HE Fan1*
    2019, 21(1):  29-36.  DOI: 10.13304/j.nykjdb.2018.0249
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    In order to explore the relationship between sugar metabolism with related enzyme activities and gene expression during the development of tobacco leaves, the contents of glucose, fructose, sucrose, reducing sugar and total soluble sugar in the development of tobacco leaves were measured; and the key enzyme activities and gene expression levels related were analyzed with the tobacco variety Qinyan 96 as material. The results showed that the contents of glucose, fructose, reducing sugar and total soluble sugar increased first and then decreased during the development of tobacco from 65 d to 115 d after transplanting. When the tobacco leaves matured (95 d after transplanting), the contents of soluble sugar (except sucrose) reached to peak value. The content of sucrose showed a bimodal fluctuation and the tobacco had the lowest sucrose content at maturity. During the topping to proper maturity of tobacco leaves, SPS and AI enzyme activities contributed the most to the accumulation of soluble sugar in tobacco leaves. When tobacco leaves were from ripe to overripe, AI was mainly involved in the regulation of sugar metabolism in tobacco leaves, while SS and SPS played important roles in the accumulation of sucrose. NtINV had a greater regulation on tobacco sugar metabolism in the stage from topping to maturity, while NtSS and NtSPS played major roles in regulating sugar metabolism in tobacco leaves from proper maturity to overripe. Sugar metabolism related genes regulated the activity of related enzymes through the molecular level and then regulated the sugar metabolism of tobacco leaves.
    Influencing Factors of Breeding Pig Living Performance Detection by B-mode Equipment
    XIA Xin, ZHAO Shuhong, HU Junyong, LIU Wanghong, NI Debin*
    2019, 21(1):  37-42.  DOI: 10.13304/j.nykjdb.2018.0105
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    In order to investigate the factors influencing pig live performance, this paper took Duroc, Landrace and Large Yorkshire as the objects and tested the back fat thickness, eye muscle depth and eye muscle area of each experimental pig by B-mode equipment. The data were analyzed by correlation analysis and variance analysis, and regression equation was built. The results showed that measurement of pig live performance by B-mode equipment was affected by breed type, final weight; related to operation skill of the tester, and also limited by the test equipment. There were no significant differences between back-fat thickness testing results by parallel viewing and cross viewing. So these 2 methods could replace each other. Above results provided scientific basis for standardizing the technical regulations for production performance measurement of breeding pigs.
    Research Progress of cfr Gene Mediated Bacteria Resistance
    XUAN Huiyong, CAI Jianxing, XIA Lining*
    2019, 21(1):  43-50.  DOI: 10.13304/j.nykjdb.2017.0903
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    With the wide use of antibiotics in veterinary clinic, the number of drug-resistant strains is increasing year by year, so the research on the mechanism of drug resistance has become a hot spot. Resistance gene cfr (chloramphenicol-florfenicol resistance) can encode to synthesize 23S rRNA methylation enzyme of bacteria. This enzyme can affect the combination of antimicrobial agents with transpeptidase and make bacteria resistant. The strains carrying cfr gene were resistant to chloramphenicol, linamide, truncated pleurodin, streptavidin A and oxazolidinone, which resulted in multidrug resistance to bacteria. Therefore, the study on the mechanism of action and transmission of drug resistance gene cfr is the key point for the backerial resistance research. In this paper, the discovery, harm, drug resistance mechanism, transmission mechanism and prevalence of multidrug resistance gene cfr in different types of bacteria were reviewed. The problems existing in present research, and suggestions for future development prospects were discussed. It provided reference and basis for the further establishment of cfr gene detection network, the development of new drugs and blocking the transmission of cfr gene.
    Research Progress on Crop Nitrogen Content Estimation Based on Remote Sensing Spectrum
    LIANG Xueying, FAN Fenglei*
    2019, 21(1):  51-61.  DOI: 10.13304/j.nykjdb.2017.0893
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    Nitrogen is one of the essential nutrients for crop growth. The deficiency or surplus of nitrogen can seriously affect crop yield and economic benefit. Timely, accurate and damage-free monitoring crop nitrogen content is of great significance for crop yield increase, rational fertilization and reduction of environmental pollution. This paper summarized the remote sensing estimation principle of crop nitrogen content, concluded the estimation methods and research progress at home and abroad. On this basis, the paper analyzed the problems existing in crop nitrogen content estimation based on remote sensing spectral data. The key points for future research was put forward, such as the improvement of model universality, the use of multi-source remote sensing data, and the monitoring of crop nitrogen content under influence of multi-factor.
    Grain Combine Harvester Cutter and Whole Machine Smoothness Simulation Analysis Based on ADAMS
    LI Jinfeng, LIU Xianxi, ZHANG Kaixing*
    2019, 21(1):  62-69.  DOI: 10.13304/j.nykjdb.2018.0075
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    In order to resolve the problems of long design cycle, low efficiency, poor product performance of grain combine-harvester cutter, and better evaluate the flatness of grain-combine harvester,this paper put forward a simulation analysis method based on ADAMS for smoothness of grain combine-harvester cutter and whole machine as well. Based on ADAMS, the paper built a drive mechanism model for the cutter and carried out simulation analysis. The time changing curve of key parameters of the cutter was obtained, thus providing references for driving mechanism improvement. At the same time, the paper also established the model of grain combine-harvester based on ADAMS, and conducted dynamic simulation on the complex obstacles roadway, thus providing favorable theoretical support for the smooth improvement of the harvester. Simulation results indicated that this method could better optimize the grain combine-harvester cutter design and complete simulation analysis on the whole machine smoothness. The above mentioned endeavors could effectively support the application of advanced design methods in the field of agricultural machinery design.
    Study on Image Segmentation Algorithm of Rice Surface Root
    LIANG Lixiu1,2, YE Junli1, WU Dan1, YANG Wanneng1*
    2019, 21(1):  70-79.  DOI: 10.13304/j.nykjdb.2018.0079
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    The quantitative studies on morphological characteristics of rice root system are of great significance for improving farmland management, rice selective breeding and genetic improvement. In recent years, along with the rapid development of phenotype technology, image processing technology could be used to measure and analyze the growth of rice root system. At the same time, combined with fertilization, irrigation, light, temperature control and environmental monitoring technology, it has become a new technological means for rice breeding and functional genomics research. And the root system image segmentation technology is one of the key foundations for the subsequent phenotypic group analysis. The segmentation is very difficult, because the rice root system images have the characteristics of low contrast, low SNR and complex texture. Aiming at this problem, the paper firstly studied the main-branch connection algorithm, local threshold segmentation algorithm based on the morphological characteristics, and adaptive threshold segmentation based on morphological characteristics, and then performed segmentation and comparison on rice root system images. The results showed that although the main-branch connection algorithm retained a large number of root details, its structure was slightly chaotic and burr phenomenon was serious; the local threshold segmentation algorithm based on morphological features could retain more root details, but the contour fracture was more severe; adaptive threshold segmentation algorithm could improve image continuity, burr phenomenon was controlled, fine roots would not be preserved. These 2 algorithms were combined into a integrated algorithm suitable for image segmentation of rice surface root system. Thus, relatively ideal segmentation results could be obtained, which would provide important basis for follow-up studies on phenotypic traits of rice root system.
    Key Area Analysis of Rice Blast in Heilongjiang Province Based on Spatial Analysis
    ZHOU Heng1, SONG Xiandong2, WANG Chunrong2, HU Yajun2,ZHANG Qifeng2, WANG Zhen2, ZHOU Dingyang1*
    2019, 21(1):  80-91.  DOI: 10.13304/j.nykjdb.2017.0900
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    Heilongjiang Province is an important rice-growing area in northern China. Rice blast is one of the major diseases affecting rice production. According to the rules of investigation and forecast of the rice blast, this paper analyzed the key occurrence counties and core occurrence areas of rice blast, and extracted key rural monitoring points of rice blast in Heilongjiang Province based on the survey data of the rice blast disease rural monitoring point during the past 5 years in Heilongjiang Province using spatial analysis. The results showed that in the past 5 years the incidence area of rice blast in Heilongjiang Province accounted for 7.3 % of the surveyed area, the onset grade was mainly level 1 and overall occurrence was lighter. The occurrence areas of rice blast were mainly distributed in Acheng, Shangzhi, Fangzheng, Yanshou, Tonghe, Yilan of Harbin City in the upper reaches of the Songhua River, the area of Fujin and Tongjiang in Jiamusi City in the lower reaches of Songhua River, the area of Zhaoyuan County of Daqing City, Nehe of Qiqihar City in Nenjiang River Basin and Beilin District of Suihua City in Hulan River basin. This paper selected several key monitoring points, including 98 leaf blast and 47 spike neck blast key monitoring points. These results provided important basis for distribution optimization of rice blast monitoring area and monitoring points in Heilongjiang Province, and also provided important support for monitoring, prevention and control of rice blast.
    Measurement and Analysis of Seed Cotton Restitution Coefficient in Point-to-plate Collision Model
    KONG Fanting, SHI Lei*, ZHANG Yutong, CHEN Changlin, SUN Yongfei, XIE Qing
    2019, 21(1):  92-99.  DOI: 10.13304/j.nykjdb.2017.0886
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    In order to establish a collision model between seed cotton and working parts of harvester and processing machine, this paper measured and analyzed restitution coefficient of seed cotton by recovery coefficient measurement device based on kinematics equation. The restitution coefficient of seed cotton was determined for different drop numbers using a drop test apparatus with drop heights ranged from 200~400 mm, and the material thickness was ranged from 2~6 mm. The collision materials were Q235 steel, aluminum alloy, organic glass and rubber. A complex orthogonal design of L8(41×24) was conducted to systematically investigate the above factors effected on restitution coefficient of ‘Zhongmian 6913’ seed cotton, harvested by brush-roller type stripper cotton harvester. The single-factor experiments were carried out under the condition of different collision materials, material thicknesses and drop heights. The results showed that the significance of above factors to restitution coefficient of seed cotton were collision material > drop height > material thickness > seed cotton number. Among them, the seed cotton number had non-significant effect on restitution coefficient. The results of single-factor experiment indicated that the order of recovery coefficient between seed cotton and collided materials was Q235 steel> aluminum alloy> organic glass> rubber. In the test scope, the restitution coefficient of seed cotton decreases almost linearly with the increase of drop height, and firstly increase significantly then slowly with the increasing thickness of collided material. The above results might provide theoretical references for rational design and simulation of seed cotton harvester and related important working parts.
    Determination and Validation of Main Parameters of Potato Tuber Morphological Structure Model
    CHEN Yuli1, YANG Ping1*, GONG Fajiang1, BI Haibin1, YUE Ting1, GAO Minghui1, CAO Hongxin2*
    2019, 21(1):  100-107.  DOI: 10.13304/j.nykjdb.2018.0028
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    Tuber morphological structure model is an important content for studying ‘function-structure potato’ model. Taking potato variety ‘Favorita’ as material, this paper carried out a field fertilizer test in potato growing seasons of 2016 and 2017. On the basis of tuber morphological-structure parameters and organ biomass data collected in the experiment of different nitrogen and potassium levels and different additional nitrogen levels, the morphological-structure models of potato tuber were built through dissecting the quantitative relationship parameters among tuber morphological-structure, organ biomass and physiological development time, and based on the internal connections of morphological-structure parameters. These models were then validated with an independent data set collected in the experiment of different based nitrogen levels. Except for index of tuber shape, tuber length, tuber width, tuber thickness, and specific tuber weight of potato had higher accuracy. These models could be used to simulate tuber structure under different fertilizer application levels.
    Evaluation on Amino Acid Nutritional Value of Xinjiang Jun Jujube
    WANG Cheng1,2, WU Dongfeng2, HE Weizhong2, WU Wenliang1*
    2019, 21(1):  108-116.  DOI: 10.13304/j.nykjdb.2017.0805
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    Amino acid variation and nutritional value of Xinjiang Jun jujube from 11 counties of Xinjiang were preliminarily verified, which provided theoretical basis for better utilization of jujube resources. Using Jiaocheng Jun jujube from Shanxi province as a control, the amino acid data of Xinjiang Jun jujube were analyzed by fuzzy recognition method, variability, and system clustering method. The results suggested the total content of amino acid in Xinjiang Jun jujube was superior to that of Jiaocheng Jun jujube. There is a big difference in amino acid composition content of Jun jujube from 12 different production areas, especially amino acids of Val, Leu, Thr, Lys and Phe. The closeness degrees of protein amino acid respect to model amino acid of different production areas were basically the same. The scores of ratio coefficient of amino acid (SRC) of Xinjiang Jun jujube were superior to that of Jiaocheng Jun jujube. The ratio coefficient of amino acid (RC) of Val from different production areas was the lowest, which mean Val was the first limited amino acid. The Jun jujubes from different production areas could be divided into three categories by cluster analysis. The better ones after protein quality evaluation were Awat, Qira and Akesu. The amino acid composition of Jun jujube protein in 11 counties of Xinjiang have their own characteristics, and the protein composition of Jun jujube in Awat, Qira and Akesu meet the nutrition requirement for protein of human, which should be further utilized.
    Heavy Metal Pollution of Cultivated Soil in Eastern China
    ZOU Mengmeng1, ZHOU Weihong1,2, ZHANG Jingjing1, LIU Ying1,
    2019, 21(1):  117-124.  DOI: 10.13304/j.nykjdb.2018.0064
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    With the rapid development of economy and society, heavy metal contamination of cultivated soil has seriously influenced food security and human health. Based on the data of heavy metal in cultivated soil in eastern China collected from documents, the degree of heavy metal pollution of cultivated land in eastern area was evaluated, by means of semi-variogram analysis, Kriging interpolation, single factor pollution index, Nemerow comprehensive pollution index, and geoaccumulation index. The conclusions showed as follows: the pollution of Pb was the most serious one; the mean value of Hg was 2.8 times higher than background value of element Hg in Chinese soil. Almost 30% of eastern area was under the middle pollution level. The pollution of Cd was the second serious one, and results of semi-variogram analysis indicated Cd was the most influenced by human activities. In addition, the mean value of Cd was 2 times higher than the background value of Cd in Chinese soil. Over 50% of eastern area was under the firing line of Cd pollution. Cultivated soil in Guangdong province may be the key region of heavy metal integrated pollution because Nemerow comprehensive pollution index in Guangdong was the highest (0.854) and Kriging interpolation revealed high value area of As, Cd, Hg, Pb distributed in Guangdong province.
    Effect of Different Close Planting Patterns on Peanut Flowering and Fruiting
    ZHANG Jun, LIU Juan, ZANG Xiuwang, HAO Xi, TANG Fengshou, DONG Wenzhao*, ZHANG Zhongxin, MIAO Lijuan, LIU Hua
    2019, 21(1):  125-131.  DOI: 10.13304/j.nykjdb.2018.0113
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    Taking peanut variety Yuanza 6 as material and adopting a split plot experiment, this paper investigated the changes in multiple indexes of summer-planting peanuts under different planting densities and planting modes, including group flowering phenology, group pod pegs formation and pod formation, yield traits etc., and discussed the effects of rational close planting on peanut group reproductive growth and yield. The results showed that comparing to planting density of 1.80×105 holes/hm2, the first flowering of peanuts grown at 2.25×105 holes/hm2 planting density would delay 1~2 d. The number of nodes on the first pair of lateral branches within 10 cm from base decreased by 10.29%; while, effective branching ratio increased by 8.50%; full fruit ratio and kernel percentage increased by 3.81% and 2.25%, respectively. The flowering time of peanuts grown in wide/narrow-row-spacing planting mode was 2~3 d ahead than that of equal-row-spacing planted peanuts, and the full fruit ratio and kernel percentage increased 3.03% and 1.58%, respectively. Comparing to single-seed planting, double-seed planting increased the effective branching ratio, full fruit ratio and kernel percentage by 10.60%, 3.70% and 1.43%, respectively. Under the condition of double-seed planting, wide/narrow-row-spacing planting mode with plant density of 2.25×105 holes/hm2, the peanuts had the highest amount of total group flowering (1 873.31×104 flowers/hm2). The numbers of group gynophores and groupfruit were also the most, reaching 1 365×104/hm2 and 488×104/hm2, respectively. Yuanza 6 was highly tolerant to compact planting. Therefore, in summer-planting appropriately increasing the planting density could effectively improve yield and perfect pod commodity.
    Comparison Analysis of Leaf Tissue Structure,Trichome and Exudates of Different Flue-cured Tobacco Varieties (Lines)
    ZHANG Siqi, HE Jia, ZHOU Fang, XU Shixiao, WANG Yuan, LIU Chao,ZHANG Anqian, CHEN Fang, SUN Zhihao, YANG Tiezhao*
    2019, 21(1):  132-141.  DOI: 10.13304/j.nykjdb.2018.0025
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    In order to investigate the difference in leaf tissue structure, trichome development and metabolism characteristics of trichome exudates of different flue-cured tobacco varieties (lines), this paper compared leaf tissue structure, trichome densities and trichome exudates of flue-cured tobacco varieties (lines) including high-aroma 8306 and Yu11 etc., by technology combining scanning electron microscopy, digital microscope and GC-MS. The results showed that: ① The long handle trichomes was predominant trichome type. The quantity of upper glandular trichomes were more than that of lower ones. The densities of short handle trichomes in Yu11, long handle trichomes in 8306, and non-glandular trichomes in K326 were higher than that of the other flue-cured tobacco varieties. ② Cembratrien-diols were main chemical compounds in trichome exudates. Labdanoids including cis-abienol and labdenediol had not been detected in these flue-cured tobacco varieties except Yu11, 8306 and 12451. ③ There was significant positive correlation between radio of palisade tissue to spongy, radio of palisade tissue to leaf thickness, trichome density and trichome exudates. On the whole, the leaf tissue structure, trichome density and trichome exudates of high-aroma flue-cured tobacco varieties (lines) were significantly higher than those of other varieties.
    Effect of Continuous Garlic Cropping on Soil Nutrients, Microbial Structure and Enzyme Activity
    LI Fengguo1, MA Longchuan2, KONG Yong3, KONG Lei4, LI Guanxi3*, ZHOU Jing3*
    2019, 21(1):  141-147.  DOI: 10.13304/j.nykjdb.2017.0904
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    In order to explore the causes of continuous cropping obstacles in garlic, this paper selected the soils in which garlic was grown for 0 year (control), 5 years, and 10 years as research objects, and researched the changes of soils nutrients, soil microorganism quantity and enzyme activities. The results showed that with the increasing of continuous cropping years, garlic yield dropped significantly compared with the control. The production decreased by 31.1% and 40.8% in 5 years and 10 years of continuous cropping soil, respectively. Soil pH increased significantly, while soil available potassium, ammonium nitrogen, nitrate nitrogen and soil organic matter decreased under long-term continuous cropping. The quantity of bacteria and actinomyces decreased, while that of fungi increased.The activities of urease, saccharase and catalase in soil tended to be decreased within 10 years of continuous cropping, indicating that long-term continuous cropping altered the soil nutrient. In conclusion, after long-term continuous cropping, the yield of garlic and enzyme activity decreased, soil pH increased, and the microorganism composition and structure were altered. It is suggested that organic fertilizer was applied to improve the enzyme activity and balance the soil microbial community structure soil property.
    Effects of Potassium Application on Growth and Bloom of Zinnia elegans
    WEI Min, ZHU Yanlin, ZHAI Yanan, LI Haiyun*
    2019, 21(1):  148-153.  DOI: 10.13304/j.nykjdb.2018.0179
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    Zinnia elegans is widely used in landscaping. Confronting potassium (K) resource shortage in China, it is necessary to clarify the optimum amount of potassium application and improve potassium utilization efficiency. This paper studied the effects of different potassium application treatments (0 mg/kg, 100 mg/kg, 200 mg/kg, 300 mg/kg and 400 mg/kg) on growth and bloom of Zinnia elegans by pot experiments, so as to provide theoretical basis for the rational application of potassium fertilizer in the cultivation process of Zinnia elegans. The results indicated that potassium application was beneficial for increasing plant height, leaf number, shoot dry weight, root dry weight, number of flowers and flower diameter. All these indexes showed a tendency of increasing first and then decreasing with the potassium level rising. When the potassium level was 300 mg/kg, these indexes reached the highest level, then declined at 400 mg/kg, but still higher than those of the control. Under this experiment condition, 300 mg/kg was the most appropriate potassium level to Zinnia elegans for its growth and bloom.
    Discrimination of Auricularia auricula from Different Producing Areas with Two-dimensional Correlation Infrared Spectroscopy
    LIN Haojian1, LIU Gang1*, YANG Weimei1, AN Ran1, OU Quanhong1, SHI Youming2
    2019, 21(1):  154-163.  DOI: 10.13304/j.nykjdb.2018.0143
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    The nutrient contents of Auricularia auricula produced at different areas are different. Therefore, it has practical value to identify the producing areas of A. auricula. This paper selected 8 kinds of A. auricula from different producing areas; analyzed them by Fourier transform infrared spectroscopy (FT-IR) two-dimensional correlation infrared (2D-IR) spectroscopy; and identified them with hierarchical cluster analysis (HCA). The results showed that fourier transformed infrared (FT-IR) spectra of 8 samples resembled extremely in holistic spectral profile, and their partial correlation coefficient was no less than 0.955, which could not be used to distinguish their origin. In their 2D-IR spectra, the numbers of auto-peaks were different in the range of 1 730~1 400 cm-1, 1 350~1 040 cm-1 and 1 030~900 cm-1. Meanwhile, there were more differences in the intensities of auto-peaks and cross-peaks. Hierarchical cluster analysis was conducted on A. auricula with FT-IR spectra in the range of 1 700~950 cm-1, and all samples from different areas were distinguished correctly. These results demonstrated that A. auricula produced at different areas could be simply and rapidly identified by 2D-IR combined with hierarchical cluster analysis without prejudice.
    Establishment and Optimization of Tissue Culture System for Nandina domestica ‘Fire power’
    ZHOU Zhijiang1,2, CHEN Mingliang2, YANG Peijuan2
    2019, 21(1):  164-168.  DOI: 10.13304/j.nykjdb.2018.0612
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    Common nandina (Nandina domestica ‘Fire power’.) belongs to Nandina genus, Berberidaceae, and it is the evergreen shrub with important ornamental value in landscape application. Traditional cultivation depends on cottage propagation, but reproduction rate is low, reproductive rate and rooting are still major problems in tissue culture of Nandina domestica ‘Fire power’. In this research, effects of different hormones on adventitious buds induction, multiplication and rooting of Nandina domestica ‘Fire power’ were studied. The results showed that MS+0.3 mg/L 6-BA + 30 g/L sugar was the best induced medium, the induction rate was 93%;the subculture medium for differentiation was MS supplemented added with 1.0 mg/L 6-BA,0.1 mg/L NAA and 30 g/L sugar, the multiplication efficient was 3.5. The optimal rooting medium was 1/2 MS added with 0.3 mg/L IBA and 20 g/L sugar,the rooting rate of plantlets could reach 97%. The seedlings soaked in carbendazim with an effective component content of 50% diluted 1 000 times for 1 h showed high survival rate and good growth. The established propagation system of Nandina domestica ‘Fire power’ had fast propagation rate, and a large number of plants could be obtained in the short term. This propagation technique provided technical support for factory production and meets the market demand.