Journal of Agricultural Science and Technology ›› 2020, Vol. 22 ›› Issue (2): 50-57.DOI: 10.13304/j.nykjdb.2019.0037

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Effects of Exogenous Melatonin on Physiological Mechanism of Drought Resistance of Tobacco Seedlings

HE Jiahao1, CHEN Jianzhong2, XU Jianqiang3, XIANG Jinyou4, YANG Yide4, ZHANG Xuewei5, NING Shanghui3, WANG Lin6, LIU Yuan7, YANG Yang4, JING Yanqiu1*, CHENG Yuyuan7*   

  1. 1.College of Tobacco, Henan Agricultural University, Zhengzhou 450002, China; 2.Nanyang Cigarette Factory, Henan China Tobacco Industry Co., Ltd., Henan Nanyang 473000, China; 3.Changde City Company of Hunan Tobacco Company, Hunan Changde 415000, China; 4.Yibin City Company of Sichuan Tobacco Company, Sichuan Yibin 644002, China; 5.Guangdong China Tobacco Industry Co., Ltd., Guangzhou 510310, China; 6.China Tobacco Hubei Industrial LLC, Wuhan 430051, China; 7.Nanyang Company of Henan Tobacco Company, Henan Nanyang 473000, China
  • Received:2019-01-04 Online:2020-02-15 Published:2019-03-28

外源褪黑素对烟草幼苗抗旱性生理机制的影响

贺嘉豪1,陈建中2,徐坚强3,向金友4,杨懿德4,张学伟5,宁尚辉3,王林6,刘园7,杨洋4,景延秋1*,程玉渊7*   

  1. 1.河南农业大学烟草学院, 郑州 450002; 2.河南中烟工业有限责任公司南阳卷烟厂, 河南 南阳 473000; 3.湖南省烟草公司常德市公司, 湖南 常德 415000; 4.四川省烟草公司宜宾市公司, 四川 宜宾 644002; 5.广东中烟工业有限责任公司, 广州 510310; 6.湖北中烟工业有限责任公司技术中心, 武汉 430051; 7.河南省烟草公司南阳市公司, 河南 南阳 473000
  • 通讯作者: *通信作者:景延秋 E-mail: jingyanqiu72t@163.com;程玉渊 E-mail: nyyyjszx@163.com
  • 作者简介:贺嘉豪 E-mail: 624749501@qq.com
  • 基金资助:
    南阳市公司重大专项(2018411301240127);湖南省烟草公司常德市公司技术开发研究项目(2018430700240157);四川省烟草公司宜宾市公司科技攻关项目(201851150024089);广东中烟工业有限责任公司项目(2019440000340016);中国烟草总公司四川省烟草公司项目(2019410102000453);湖北中烟工业有限责任公司项目(2018420000340277)。

Abstract: In order to study the effects of exogenous melatonin on tobacco seedlings under different drought stress levels, the growth, photosynthetic characteristics, antioxidant enzymes activities and osmotic regulatory substances contents of tobacco seedlings under drought stress were investigated after sprayed 100 μmol·L-1 melatonin. The results showed that plant growth was inhibited by drought stress; spraying melatonin alleviated the growth of tobacco plants inhibited by drought stress. Melatonin spraying improved the chlorophyll content and net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr) of tobacco plant leaves, decreased the intercellular CO2 concentration (Ci). Moreover, melatonin application enhanced the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), reduced O-2 and malondialdehyde (MDA) content, and increased the accumulation of osmotic regulation substances significantly. Therefore melatonin application can improve drought stress resistance of tobacco seedling. The present research provided reference for studying the mechanism of effects of melatonin on drought resistance in tobacco seedlings.

Key words: melatonin, tobacco seedlings, drought stress, physiological mechanism

摘要: 为研究喷施外源褪黑素对烟草幼苗不同程度干旱胁迫条件下的影响效应。以烟草幼苗为试验材料,探究喷施100 μmol·L-1外源褪黑素对干旱胁迫下幼苗的生长、光合特性、抗氧化酶活性以及渗透调节物质含量的影响。结果表明,干旱严重影响植物生长,喷施褪黑素缓解了干旱胁迫造成的植株生长抑制,并通过提高叶片叶绿素含量和净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr),降低胞间CO2浓度(Ci),同时增强超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性,减少O-2和丙二醛(MDA)的含量,显著增加渗透调节物质的积累,以提高烟草幼苗的抗干旱胁迫能力,结果为褪黑素对烟草幼苗的抗旱性影响机理提供依据。

关键词: 褪黑素, 烟草幼苗, 干旱胁迫, 生理机制