Journal of Agricultural Science and Technology ›› 2022, Vol. 24 ›› Issue (2): 210-217.DOI: 10.13304/j.nykjdb.2021.0094

• BIO-MANUFACTURING & RESOURCE AND ECOLOGY • Previous Articles    

Cold Resistance of Alfalfa Root Neck Under Different Phosphate Fertilizer Treatments and Changes of Carbohydrate Under Low Temperature Stress

Weidong CHEN1(), Yuxia ZHANG1(), Baiming CONG2, Quanchao XIA1, Yonglei TIAN3, Qingxin ZHANG1, Xiaoyan DU1   

  1. 1.Inner Mongolia Autonomous Region Forage Crop Engineering Technology Research Center,Agricultural College of Inner Mongolia Minzu University,Inner Mongolia Tongliao 028041,China
    2.Tongliao Institute of Animal Husbandry and Veterinary Science,Inner Mongolia Tongliao 028000,China
    3.Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences,Hohhot 010010,China
  • Received:2021-01-27 Accepted:2021-06-01 Online:2022-02-15 Published:2022-02-22
  • Contact: Yuxia ZHANG

不同磷肥处理下苜蓿根颈抗寒性及在低温胁迫下糖类物质变化

陈卫东1(), 张玉霞1(), 丛百明2, 夏全超1, 田永雷3, 张庆昕1, 杜晓艳1   

  1. 1.内蒙古民族大学农学院,内蒙古自治区饲用作物工程中心,内蒙古 通辽 028041
    2.内蒙古通辽市畜牧兽医科学研究所,内蒙古 通辽 028000
    3.内蒙古自治区农牧业科学院,呼和浩特 010010
  • 通讯作者: 张玉霞
  • 作者简介:陈卫东 E-mail:1416386697@qq.com
  • 基金资助:
    内蒙古自然科学研究基金项目(2020MS03081);内蒙古自治区科技储备项目(2018MDCB032);荒漠草原区草畜一体化关键技术集成与示范项目(2019GG245)

Abstract:

In order to explore the mechanism of phosphate fertilizer type and application rate regulating the cold resistance and sugar content of alfalfa, taking‘Gongnong No. 1’ alfalfa was as the material, and a two-factor (phosphorous fertilizer type and fertilizer amount) randomized block experiment design was set, which two types of phosphorous fertilizer type (heavy superphosphate and diammonium phosphate) were used and the applying amounts were set according to 0, 100, 200, 300, 400 kg·hm-2 (calculated as P2O5) in autumn. The root neck vitality and contents of soluble sugar, sucrose, fructose, starch in root neck and the tap root 1 cm below the root neck under low temperature storage (4 ℃) and low temperature freezing (?20 ℃) were measured. The results showed that, compared with 4 ℃ treatment, the root neck vitality and content of soluble sugar were higher and the content of starch was lower under ?20 ℃ treatment. The root neck vitalities and contents of fructose in heavy superphosphate treatments under ?20 ℃ treatment were higher than those in diammonium phosphate treatments, but the starch contents were conversely. And the Optimum amounts of two types of phosphate fertilizers were 300 kg·hm-2 (P2O5) with higher contents of soluble sugar and fructose in the root neck of alfalfa. In conclusion, a moderate amount of phosphorus could promote the conversion from starch to soluble sugar in alfalfa root neck and improve the cold resistance of alfalfa. Application of superphosphate with 300 kg·hm-2 (P2O5) in Horqin sandy land was conducive to the safe overwintering of alfalfa.

Key words: phosphate fertilizer, alfalfa, cold resistance, sugar substance

摘要:

为探究磷肥种类及施用量调控紫花苜蓿抗寒性及糖类物质变化的机制,以‘公农1号’ 紫花苜蓿为材料,采用二因素(磷肥种类和施肥量)随机区组试验设计,设置分别施用重过磷酸钙和磷酸二铵0、100、200、300和400 kg·hm-2(以P2O5计),于越冬前期将不同处理的根颈收获后分别进行冷藏(4 ℃)和冷冻(?20 ℃)处理,对低温处理后根颈(根颈及根颈以下主根1 cm)活力及糖类物质(可溶性糖、蔗糖、果糖、淀粉)含量进行测定。结果表明,不同磷肥处理苜蓿越冬材料冷冻处理后的根颈活力和淀粉含量均低于冷藏处理,但可溶性糖、蔗糖和果糖含量高于冷藏处理。在冷冻处理中,施用重过磷酸钙处理苜蓿的根颈活力及果糖含量显著高于磷酸二铵处理,但淀粉含量显著低于磷酸二铵处理;2种磷肥均以300 kg·hm-2处理苜蓿根颈的可溶性糖和果糖含量最高。由此表明,增施磷肥有利于苜蓿根颈中淀粉在低温时转化成蔗糖、果糖等可溶性糖类物质,提高苜蓿的抗寒性;在科尔沁沙地施用重过磷酸钙,且施用量为300 kg·hm-2(P2O5)时有利于苜蓿安全越冬。

关键词: 磷肥, 苜蓿, 抗寒性, 糖类物质

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