Abstract: To study the effect of Trichoderma harzianum on the metabolic pathways related to disease resistance of tobacco Phytophthora nicotianae, NC89 was as the test material, and four treatments were set: ① inoculation with clean water (NC89CK), ② inoculation with Phytophthora nicotianae (NC89_P), ③ inoculation of Trichoderma harzianum (NC89_T); ④ inoculation with Phytophthora nicotianae after inoculation with Trichoderma harzianum (NC89_T_P). Liquid chromatography-mass spectrometer (LC/MS) technology was used to analyze the changes of different metabolic pathways related to disease resistance of different treatments. The result showed Trichoderma harzianum had a good biocontrol effect on Phytophthora nicotianae. The results of metabolomics analysis among all treatments showed that the contents of substances, such as fatty acids and their derivatives, flavonoids, terpenoids, phenylalanine and its secondary metabolites, pantothenic acid and other substances in the metabolic pathway, were significant differences. There were 20 metabolic pathways related to disease resistance. And a number of significantly different metabolites and their enriched metabolic pathways were screened, which laid the foundation for further exploration of the  mechanism of Trichoderma harzianum against Phytophthora nicotianae.

Key words: tobacco black shank disease, Trichoderma harzianum, metabolomics, LC/MS

摘要： 为研究哈茨木霉菌对NC89接种烟草疫霉菌前后抗病相关代谢途径的影响，以烟草品种NC89为试验材料，设置四个处理：接种清水（NC89CK）、接种烟草疫霉菌谷（NC89_P）、接种哈茨木霉菌（NC89_T）、接种哈茨木霉菌后接种烟草疫霉菌谷（NC89_T_P），利用超高效液相色谱与质谱联用技术（liquid chromatography-mass spectrometer，LC/MS）比较各处理抗病相关代谢通路的变化差异。结果表明，哈茨木霉菌对烟草疫霉菌有着较好的生防作用。代谢组学分析表明，筛选到893个差异代谢产物及其富集的代谢通路，涉及抗病相关的代谢途径20条；各处理间脂肪酸及其衍生物、黄酮和类黄酮、萜类、苯丙氨酸及其次生代谢物和泛酸等物质的含量存在显著差异，为进一步探究哈茨木霉菌对烟草疫霉菌的抑制机理奠定了基础。

关键词: 烟草黑胫病, 哈茨木霉, 代谢组学, LC/MS