Genome-wide Identification of GELP Family Genes in Cotton and Expression Analysis Under Stress

doi:10.13304/j.nykjdb.2021.0389

Abstract

Abstract:

GDSL-type esterase/lipase proteins （GELPs） belong to lipolytic enzyme， which contain conserved GDSL motif at their N-terminus. GELPs play crucial roles in growth and development， stress responses and pathogen defense. However， the identification and functional analysis of GELP genes are hardly explored in cotton. This study identified 210 GELP genes in the G. hirsutum L genome and they were divided into ten clusters （A～J） by phylogenetic analysis. The GhGELPs were universally and unevenly distributed on all 26 chromosomes， the majority of which were located on chromosome ends. Gene structure analysis revealed that more than 66.7% of GhGELP genes were interrupted by 4 introns and composed of 5 exons in their coding regions. Further， the presence of cis-elements potentially related to the hormonal signal and stresses responses were surveyed， and a total of seven types cis-elements in the promoters were predicted. Finally， RNA-seq data were used to investigate the expression profiles of GhGELP genes under different biotic and abiotic stress treatments. The expression analysis revealed that majority of GhGELPs were down-regulated in response to cold， hot， salt， PEG and Verticilliumdahliae infection. In addition， some members could be simultaneously induced by multiple abiotic stresses. This paper identified the family members of cotton GhGELP genes， which provided theoretical basis for future studies of cotton GELP genes.

Key words: Gossypiumhirsutum L．, GELP gene family, gene identification, phylogenetic analysis, stress response

摘要：

GELP型脂肪酶（GDSL-type esterase/lipase proteins，GELPs）是一类N端含有GDSL-motif的脂质水解酶，在植物生长发育、应激反应及病原菌防御中均发挥非常重要的作用。基于最新发布的陆地棉基因组数据，从全基因组水平上鉴定出210个陆地棉GELP基因，根据系统发育关系将其分为10个亚家族（A～J）。这些GhGELP基因不均匀地分布在26条染色体上，且主要分布在染色体两端。基因结构分析表明，高达66.7%的GhGELP基因被4个内含子打断，其编码区域由5个外显子组成。分析发现，GhGELP基因启动子区域共存在7种激素和逆境胁迫响应元件。最后，利用RNA-seq数据研究了GhGELP基因在黄萎病菌和多种非生物胁迫处理下的表达谱。表达分析显示，大多数GhGELP基因响应黄萎病菌诱导后表达量下调，受非生物胁迫诱导后亦呈下调表达趋势，且一些成员可同时响应多种非生物胁迫。对棉花GELP基因家族进行了鉴定和分析，为后续研究其功能提供了一定的理论依据。

关键词: 陆地棉, GELP基因家族, 基因鉴定, 系统发育分析, 胁迫响应

CLC Number:

S562

Shaojing MO, Zhicheng WANG, Xingfen WANG, Zhengwen LIU, Liqiang WU, Guiyin ZHANG, Zhiying MA, Yan ZHANG, Huijun DUAN. Genome-wide Identification of GELP Family Genes in Cotton and Expression Analysis Under Stress[J]. Journal of Agricultural Science and Technology, 2022, 24(2): 93-103.

默韶京, 王志城, 王省芬, 刘正文, 吴立强, 张桂寅, 马峙英, 张艳, 段会军. 陆地棉GELP家族基因鉴定及其响应胁迫的表达分析[J]. 中国农业科技导报, 2022, 24(2): 93-103.

Figures/Tables 6

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基因名称 Gene name	盐 Salt	PEG	热 Hot	冷 Cold	基因名称 Gene name	盐 Salt	PEG	热 Hot	冷 Cold
GhGELP1	√	√			GhGELP109		√	√
GhGELP2	√	√	√	√	GhGELP114	√	√	√	√
GhGELP5	√	√	√	√	GhGELP115		√	√
GhGELP6		√			GhGELP118	√	√	√	√
GhGELP7		√	√		GhGELP119	√	√	√	√
GhGELP9			√	√	GhGELP120			√
GhGELP10	√		√		GhGELP124		√
GhGELP15			√		GhGELP127	√	√	√
GhGELP26	√	√	√		GhGELP128				√
GhGELP29	√	√	√	√	GhGELP131		√	√
GhGELP30	√	√	√		GhGELP133	√	√	√	√
GhGELP32	√	√		√	GhGELP136			√	√
GhGELP33	√			√	GhGELP137	√	√	√	√
GhGELP37	√	√	√	√	GhGELP138			√	√
GhGELP38			√	√	GhGELP139			√	√
GhGELP41	√	√			GhGELP144	√	√		√
GhGELP42	√	√	√		GhGELP146	√	√
GhGELP43	√	√		√	GhGELP149	√	√	√	√
GhGELP46	√	√	√		GhGELP153	√	√
GhGELP50	√	√			GhGELP154	√	√
GhGELP51	√	√	√	√	GhGELP157	√
GhGELP55		√			GhGELP163		√
GhGELP59	√	√			GhGELP164	√	√
GhGELP60	√	√			GhGELP165	√	√
GhGELP69	√	√	√		GhGELP175	√	√	√
GhGELP71	√		√	√	GhGELP176	√
GhGELP72	√		√	√	GhGELP177	√	√
GhGELP79	√				GhGELP178	√	√
GhGELP81	√	√		√	GhGELP185	√	√	√	√
GhGELP84	√	√			GhGELP186	√	√		√
GhGELP85	√	√		√	GhGELP187		√		√
GhGELP86	√			√	GhGELP190	√	√
GhGELP90	√	√	√	√	GhGELP191	√	√		√
GhGELP92				√	GhGELP192			√	√
GhGELP98	√				GhGELP197	√	√
GhGELP99	√				GhGELP199				√
GhGELP104	√	√	√	√	GhGELP205	√	√		√
GhGELP107	√				GhGELP207	√	√	√
GhGELP108		√			GhGELP208				√

基因名称 Gene name	盐 Salt	PEG	热 Hot	冷 Cold	基因名称 Gene name	盐 Salt	PEG	热 Hot	冷 Cold
GhGELP1	√	√			GhGELP109		√	√
GhGELP2	√	√	√	√	GhGELP114	√	√	√	√
GhGELP5	√	√	√	√	GhGELP115		√	√
GhGELP6		√			GhGELP118	√	√	√	√
GhGELP7		√	√		GhGELP119	√	√	√	√
GhGELP9			√	√	GhGELP120			√
GhGELP10	√		√		GhGELP124		√
GhGELP15			√		GhGELP127	√	√	√
GhGELP26	√	√	√		GhGELP128				√
GhGELP29	√	√	√	√	GhGELP131		√	√
GhGELP30	√	√	√		GhGELP133	√	√	√	√
GhGELP32	√	√		√	GhGELP136			√	√
GhGELP33	√			√	GhGELP137	√	√	√	√
GhGELP37	√	√	√	√	GhGELP138			√	√
GhGELP38			√	√	GhGELP139			√	√
GhGELP41	√	√			GhGELP144	√	√		√
GhGELP42	√	√	√		GhGELP146	√	√
GhGELP43	√	√		√	GhGELP149	√	√	√	√
GhGELP46	√	√	√		GhGELP153	√	√
GhGELP50	√	√			GhGELP154	√	√
GhGELP51	√	√	√	√	GhGELP157	√
GhGELP55		√			GhGELP163		√
GhGELP59	√	√			GhGELP164	√	√
GhGELP60	√	√			GhGELP165	√	√
GhGELP69	√	√	√		GhGELP175	√	√	√
GhGELP71	√		√	√	GhGELP176	√
GhGELP72	√		√	√	GhGELP177	√	√
GhGELP79	√				GhGELP178	√	√
GhGELP81	√	√		√	GhGELP185	√	√	√	√
GhGELP84	√	√			GhGELP186	√	√		√
GhGELP85	√	√		√	GhGELP187		√		√
GhGELP86	√			√	GhGELP190	√	√
GhGELP90	√	√	√	√	GhGELP191	√	√		√
GhGELP92				√	GhGELP192			√	√
GhGELP98	√				GhGELP197	√	√
GhGELP99	√				GhGELP199				√
GhGELP104	√	√	√	√	GhGELP205	√	√		√
GhGELP107	√				GhGELP207	√	√	√
GhGELP108		√			GhGELP208				√

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