Research Progress on Plant RNA-dependant RNA Polymerase

doi:10.3969/j.issn.1008-0864.2011.06.08

›› 2011, Vol. 13 ›› Issue (6): 46-53.DOI: 10.3969/j.issn.1008-0864.2011.06.08

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Research Progress on Plant RNA-dependant RNA Polymerase

XU Tao, WANG Lei

(Biotechnology Research Institution, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:2011-05-09 Revised:2011-05-26 Online:2011-12-15 Published:2011-10-20

植物中依赖于RNA的RNA聚合酶研究进展

徐涛,王磊

(中国农业科学院生物技术研究所, 北京 100081)

通讯作者: 王磊,研究员,博士,主要从事植物基因工程研究。Tel:010-82106134;E-mail:wanglei@caas.net.cn
作者简介:徐涛,硕士研究生,研究方向为植物基因沉默。
基金资助:
国家自然科学基金(31000576);国家973计划项目(2007CB109004);国家新品种培育转基因重大专项(2009ZX08009-16B)资助。

Abstract

Abstract:

RNA-dependant RNA polymerase (RDR) could synthesize RNA strand complementary using single-stranded RNA as template to produce double-stranded RNA. Then the double-stranded RNAs are processed into 20~24 nt small interfering RNAs (siRNA) by DCL similar to cellular RNaseⅢ-like, which could transcriptionally or posttranscriptionally repress the expression of target genes. Through gene silencing pathway, RDR participates in multiple biological processes, such as plant developmental regulation, stress response and epigenetic modification. Thorough understanding about the expression profile, biochemical property and biological function of plant RDR will benefit the development and utilization of plant gene silencing.

Key words: gene silencing, RDR-dependant RNA polymerase, small RNA, developmental regulation, stress response, DNA ethylation

摘要：

依赖于RNA的RNA聚合酶(RDR)能够以单链RNA为模版合成互补RNA链,产生双链RNA。双链RNA在细胞内被类似RNaseⅢ的酶DCL加工成20～24 nt的小分子干扰RNA(siRNA)。siRNA可以在转录水平或转录后水平抑制靶基因的表达。RDR通过基因沉默途径参与植物的生长发育调节、逆境应答以及表观遗传修饰等许多生物学过程。加深对植物RDR表达模式、生化活性及生物学功能等方面的理解将有利于植物基因沉默的发展和运用。

关键词: 基因沉默;依赖于RNA的RNA聚合酶;小RNA;发育调节;逆境应答;DNA甲基化

CLC Number:

Q756

XU Tao, WANG Lei. Research Progress on Plant RNA-dependant RNA Polymerase[J]. , 2011, 13(6): 46-53.

徐涛,王磊. 植物中依赖于RNA的RNA聚合酶研究进展[J]. , 2011, 13(6): 46-53.

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Research Progress on Plant RNA-dependant RNA Polymerase

植物中依赖于RNA的RNA聚合酶研究进展

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