Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (7): 77-86.DOI: 10.13304/j.nykjdb.2022.0170

• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles     Next Articles

Effects of Overexpression of veA Gene on Secondary Metabolism of Eurotium cristatus

Yunsheng WANG(), Yincui CHEN, Zai CHENG, Jin ZHANG, Chuanbo ZHANG()   

  1. College of Life Sciences,Guizhou Normal University,Guiyang 550025,China
  • Received:2022-03-07 Accepted:2022-04-20 Online:2023-07-15 Published:2023-08-25
  • Contact: Chuanbo ZHANG


王云胜(), 陈银翠, 程在, 张锦, 张传博()   

  1. 贵州师范大学生命科学学院,贵阳 550025
  • 通讯作者: 张传博
  • 作者简介:王云胜
  • 基金资助:


Eurotium cristatus is a dominant fungus in the process of Fuzhuan tea, the extracellular enzymes and abundance of secondary metabolites affect the quality and taste of finished tea. To explore the regulation mechanism of veA on secondary metabolism of Eurotium cristatus, the veA overexpressed mutant was successfully constructed by seamless cloning and agrobacterium transformation technology, and the effect of overexpression of veA on E. cristatus was evaluated by high performance liquid chromatography (HPLC) and transcriptomic sequencing technology. The results showed that the contents of various compounds in the fermentation products of the mutant strain were significantly different from those of the wild type, 3 new compounds were detected and the absorption peak of compound 1 increased by 4.09 times. Also, there were significant differences in gene expression profiles between wild type and mutant strain. A total of 737 differentially expressed genes (DEGs) were detected, among which 282 genes were significantly up-regulated and 455 genes were significantly down-regulated. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway showed that DEGs were mainly enriched in amino acid metabolism, proteasome and terpenoid backbone biosynthesis. AntiSMASH software annotated 545 genes related to secondary metabolite synthesis, of which 10.64% genes were significantly differentially expressed between wild type and mutant strain, including polypentenyl synthase gene, isoprene diphosphate δ-isomerase gene and farniki pyrophosphate synthase gene were significantly up-regulated. It suggested that overexpression of veA activated some secondary metabolite genes, which provided new insights and methods for further mining novel compounds of E. cristatus.

Key words: Eurotiumcristatus, veA, overexpression, transcriptome, secondary metabolism


冠突散囊菌是茯砖茶加工过程中的优势益生真菌,其产生的胞外酶和丰富的次生代谢产物影响成品茶的品质和口感。为探究veA对冠突散囊菌次级代谢的调控机制,使用无缝克隆及农杆菌转化技术构建了冠突散囊菌veA基因过表达突变株,使用高效液相色谱技术(high performance liquid chromatography,HPLC)和转录组测序初步解析过表达veA对冠突散囊菌次级代谢相关基因表达的影响。结果表明,与野生型冠突散囊菌相比,突变株发酵产物中多种化合物含量具有明显变化,物质吸收峰1的相对峰面积提高了4.09倍,且检测到3种新的物质吸收峰。转录组测序结果表明,与野生型相比,突变株的基因表达谱具有较大差异,共检测出737个显著差异表达基因(differentially expressed genes,DEGs),其中,282个基因显著上调表达,455个基因显著下调表达。DEGs主要富集在氨基酸代谢、蛋白酶和萜类化合物合成等通路。使用antiSMASH软件注释到545个次级代谢产物合成基因,10.64%的基因表达存在显著差异,其中,聚戊烯基合成酶基因、异戊二烯基二磷酸δ-异构酶基因和法尼基焦磷酸合成酶基因均显著上调表达。上述结果表明VeA能够激活一些次级代谢相关基因的表达,为冠突散囊菌的发酵应用和新颖次级代谢产物的挖掘提供了新的见解和方法。

关键词: 冠突散囊菌, veA基因, 过表达, 转录组, 次级代谢

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