Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (7): 77-86.DOI: 10.13304/j.nykjdb.2022.0170
• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles Next Articles
Yunsheng WANG(), Yincui CHEN, Zai CHENG, Jin ZHANG, Chuanbo ZHANG(
)
Received:
2022-03-07
Accepted:
2022-04-20
Online:
2023-07-15
Published:
2023-08-25
Contact:
Chuanbo ZHANG
通讯作者:
张传博
作者简介:
王云胜 E-mail:1710818668@qq.com;
基金资助:
CLC Number:
Yunsheng WANG, Yincui CHEN, Zai CHENG, Jin ZHANG, Chuanbo ZHANG. Effects of Overexpression of veA Gene on Secondary Metabolism of Eurotium cristatus[J]. Journal of Agricultural Science and Technology, 2023, 25(7): 77-86.
王云胜, 陈银翠, 程在, 张锦, 张传博. 过表达veA基因对冠突散囊菌次级代谢的影响[J]. 中国农业科技导报, 2023, 25(7): 77-86.
引物 Primer | 序列 Sequence (5’-3’) |
---|---|
veAF | AGACATCACCATGGTAGATCTATGGCGACGCGAATGCCC |
veAR | GGGGAAATTCGAGCTGGTCACCTTAACTAGAAAAAGCCGGCGG |
GAPDHF | CTGCCGTATCGAGAAGGGTG |
GAPDHR | GATGAAGTTGGGGTTGAGGG |
QveAF | CGACATCACCTTTGCCTAC |
QveAR | TCGGGAAAGATGAAGTAGC |
Table 1 Primers used in this study
引物 Primer | 序列 Sequence (5’-3’) |
---|---|
veAF | AGACATCACCATGGTAGATCTATGGCGACGCGAATGCCC |
veAR | GGGGAAATTCGAGCTGGTCACCTTAACTAGAAAAAGCCGGCGG |
GAPDHF | CTGCCGTATCGAGAAGGGTG |
GAPDHR | GATGAAGTTGGGGTTGAGGG |
QveAF | CGACATCACCTTTGCCTAC |
QveAR | TCGGGAAAGATGAAGTAGC |
样品 Sample | 原始数据 Raw reads | 质控数据 Clean reads | 错配率 Error rate/% | Q20/% | Q30/% |
---|---|---|---|---|---|
OE::veA1_1 | 53 215 236 | 52 880 844 | 0.023 9 | 98.44 | 95.28 |
OE::veA1_2 | 58 759 294 | 58 355 148 | 0.024 0 | 98.4 | 95.21 |
OE::veA1_3 | 56 979 242 | 56 535 564 | 0.024 1 | 98.36 | 95.11 |
WT1 | 52 014 002 | 51 659 080 | 0.024 0 | 98.41 | 95.24 |
WT2 | 57 594 222 | 57 246 210 | 0.023 9 | 98.43 | 95.26 |
WT3 | 58 672 768 | 58 282 756 | 0.023 9 | 98.42 | 95.25 |
Table 2 Transcriptome sequencing data statistics
样品 Sample | 原始数据 Raw reads | 质控数据 Clean reads | 错配率 Error rate/% | Q20/% | Q30/% |
---|---|---|---|---|---|
OE::veA1_1 | 53 215 236 | 52 880 844 | 0.023 9 | 98.44 | 95.28 |
OE::veA1_2 | 58 759 294 | 58 355 148 | 0.024 0 | 98.4 | 95.21 |
OE::veA1_3 | 56 979 242 | 56 535 564 | 0.024 1 | 98.36 | 95.11 |
WT1 | 52 014 002 | 51 659 080 | 0.024 0 | 98.41 | 95.24 |
WT2 | 57 594 222 | 57 246 210 | 0.023 9 | 98.43 | 95.26 |
WT3 | 58 672 768 | 58 282 756 | 0.023 9 | 98.42 | 95.25 |
Fig. 3 Differentially expressed genes between WT and OE::veA1A: Histogram of differentially expressed genes; B: Volcano map of differentially expressed genes
基因编号 Gene ID | 功能注释 Function annotation | 变化倍数 Fold change | 调控 Regulate |
---|---|---|---|
SI65_05759 | 氧化还原酶 Flavoprotein-ubiquinone oxidoreductase | 1.01ns | 上调Up |
SI65_05760 | 反转录酶 Reverse transcriptase | 0.61ns | 下调Down |
SI65_05761 | TATA框结合蛋白 TATA-box-binding protein | 0.72ns | 下调Down |
SI65_05762 | L-甲基哌啶氧化酶 L-pipecolate oxidase | 1.50* | 上调Up |
SI65_05763 | 甲基转移酶 Methyltransferase | 1.05ns | 上调Up |
SI65_05764 | 假设蛋白 Hypothetical protein | 1.45ns | 上调Up |
SI65_05765 | C6指域转录因子 C6 finger domain transcription factor | 1.25ns | 上调Up |
SI65_05766 | NADH-生化色素b5还原酶 NADH-cytochrome b5 reductase | 1.27ns | 上调 Up |
SI65_05767 | 甲基转移酶 Methyltransferase | 1.72* | 上调Up |
SI65_05768 | 羧基酸合成酶 Carboxylic acid synthase | 2.02* | 上调Up |
SI65_05769 | 金属化合物-β-内酰胺酶 Metallo-beta-lactamase | 2.31* | 上调Up |
SI65_05770 | 细胞色素P450单加氧酶 Cytochrome P450 monooxygenase | 1.92ns | 上调Up |
SI65_05771 | 细胞色素P450单加氧酶 Cytochrome P450 monooxygenase | 2.47* | 上调 Up |
SI65_05772 | 氧化还原酶 Oxidoreductase | 2.67* | Up 上调 |
SI65_05773 | 乙酰基转移酶 Acetyltransferase | 2.74* | Up 上调 |
SI65_05774 | 假定蛋白 Hypothetical protein | NA | NA |
SI65_05775 | 假定蛋白 Hypothetical protein | 0.55* | Down 下调 |
SI65_05776 | 转录因子 Transcription factor | 0.64* | Down 下调 |
Table 3 Gene expression and functional annotation in secondary metabolite gene cluster 32
基因编号 Gene ID | 功能注释 Function annotation | 变化倍数 Fold change | 调控 Regulate |
---|---|---|---|
SI65_05759 | 氧化还原酶 Flavoprotein-ubiquinone oxidoreductase | 1.01ns | 上调Up |
SI65_05760 | 反转录酶 Reverse transcriptase | 0.61ns | 下调Down |
SI65_05761 | TATA框结合蛋白 TATA-box-binding protein | 0.72ns | 下调Down |
SI65_05762 | L-甲基哌啶氧化酶 L-pipecolate oxidase | 1.50* | 上调Up |
SI65_05763 | 甲基转移酶 Methyltransferase | 1.05ns | 上调Up |
SI65_05764 | 假设蛋白 Hypothetical protein | 1.45ns | 上调Up |
SI65_05765 | C6指域转录因子 C6 finger domain transcription factor | 1.25ns | 上调Up |
SI65_05766 | NADH-生化色素b5还原酶 NADH-cytochrome b5 reductase | 1.27ns | 上调 Up |
SI65_05767 | 甲基转移酶 Methyltransferase | 1.72* | 上调Up |
SI65_05768 | 羧基酸合成酶 Carboxylic acid synthase | 2.02* | 上调Up |
SI65_05769 | 金属化合物-β-内酰胺酶 Metallo-beta-lactamase | 2.31* | 上调Up |
SI65_05770 | 细胞色素P450单加氧酶 Cytochrome P450 monooxygenase | 1.92ns | 上调Up |
SI65_05771 | 细胞色素P450单加氧酶 Cytochrome P450 monooxygenase | 2.47* | 上调 Up |
SI65_05772 | 氧化还原酶 Oxidoreductase | 2.67* | Up 上调 |
SI65_05773 | 乙酰基转移酶 Acetyltransferase | 2.74* | Up 上调 |
SI65_05774 | 假定蛋白 Hypothetical protein | NA | NA |
SI65_05775 | 假定蛋白 Hypothetical protein | 0.55* | Down 下调 |
SI65_05776 | 转录因子 Transcription factor | 0.64* | Down 下调 |
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