Journal of Agricultural Science and Technology ›› 2022, Vol. 24 ›› Issue (10): 35-43.DOI: 10.13304/j.nykjdb.2022.0198
• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles Next Articles
Ruifeng GUO1(), Yuemei REN1(
), Zhong YANG1, Guishan LIU2, Guangbing REN1, Shou ZHANG1, Wenjuan ZHU1
Received:
2022-03-15
Accepted:
2022-05-28
Online:
2022-10-15
Published:
2022-10-25
Contact:
Yuemei REN
郭瑞锋1(), 任月梅1(
), 杨忠1, 刘贵山2, 任广兵1, 张绶1, 朱文娟1
通讯作者:
任月梅
作者简介:
郭瑞锋 E-mail:guoruifeng229@126.com;
基金资助:
CLC Number:
Ruifeng GUO, Yuemei REN, Zhong YANG, Guishan LIU, Guangbing REN, Shou ZHANG, Wenjuan ZHU. Transcriptomic Analysis of Mechanism of Foxtail Millet Male Infertility Induced by Glyphosate Ammonium Salt[J]. Journal of Agricultural Science and Technology, 2022, 24(10): 35-43.
郭瑞锋, 任月梅, 杨忠, 刘贵山, 任广兵, 张绶, 朱文娟. 草甘膦铵盐诱导谷子雄性不育的转录组分析[J]. 中国农业科技导报, 2022, 24(10): 35-43.
样品编号 Sample code | 高质量序列 Clean read | 高质量序列碱基数 Base number of clean read | Q20/% | Q30/% | 比对序列 Total mapped read | |||
---|---|---|---|---|---|---|---|---|
数量 Number | 占比 Percentage/% | 数量 Number | 占比 Percentage/% | |||||
数量 Number | 占比 Percentage/% | |||||||
S1 | 42 672 248 | 98.99 | 6 424 467 638 | 98.69 | 96.12 | 90.79 | 36 768 433 | 86.16 |
S2 | 41 691 824 | 98.98 | 6 276 055 582 | 98.67 | 96.07 | 90.71 | 34 558 553 | 82.89 |
P1 | 44 638 924 | 99.18 | 6 719 144 472 | 98.87 | 96.36 | 91.24 | 35 018 847 | 78.45 |
P2 | 44 681 598 | 98.87 | 6 729 889 484 | 98.62 | 95.96 | 90.52 | 37 171 009 | 83.19 |
CK1 | 45 815 562 | 98.94 | 6 896 999 720 | 98.63 | 95.96 | 90.48 | 33 550 009 | 73.23 |
CK2 | 45 088 252 | 99.07 | 6 786 443 052 | 98.75 | 96.02 | 90.66 | 35 095 278 | 77.84 |
Table 1 Raw data statistics
样品编号 Sample code | 高质量序列 Clean read | 高质量序列碱基数 Base number of clean read | Q20/% | Q30/% | 比对序列 Total mapped read | |||
---|---|---|---|---|---|---|---|---|
数量 Number | 占比 Percentage/% | 数量 Number | 占比 Percentage/% | |||||
数量 Number | 占比 Percentage/% | |||||||
S1 | 42 672 248 | 98.99 | 6 424 467 638 | 98.69 | 96.12 | 90.79 | 36 768 433 | 86.16 |
S2 | 41 691 824 | 98.98 | 6 276 055 582 | 98.67 | 96.07 | 90.71 | 34 558 553 | 82.89 |
P1 | 44 638 924 | 99.18 | 6 719 144 472 | 98.87 | 96.36 | 91.24 | 35 018 847 | 78.45 |
P2 | 44 681 598 | 98.87 | 6 729 889 484 | 98.62 | 95.96 | 90.52 | 37 171 009 | 83.19 |
CK1 | 45 815 562 | 98.94 | 6 896 999 720 | 98.63 | 95.96 | 90.48 | 33 550 009 | 73.23 |
CK2 | 45 088 252 | 99.07 | 6 786 443 052 | 98.75 | 96.02 | 90.66 | 35 095 278 | 77.84 |
样本对 Paired samples | 差异表达基因数量 Number of differentially expressed genes | ||
---|---|---|---|
上调Up | 下调Down | 总计Total | |
S1/CK1 | 296 | 501 | 797 |
S1/P1 | 56 | 113 | 169 |
P1/CK1 | 1 513 | 1 559 | 3 072 |
Table 2 Number of differentially expressed genes
样本对 Paired samples | 差异表达基因数量 Number of differentially expressed genes | ||
---|---|---|---|
上调Up | 下调Down | 总计Total | |
S1/CK1 | 296 | 501 | 797 |
S1/P1 | 56 | 113 | 169 |
P1/CK1 | 1 513 | 1 559 | 3 072 |
类别 Catergory | GO条目 GO term | 代谢节点编号 Metabolic node number | 差异表达基因数量 Number of DEGs | P值(校正后) P-value (corrected) | ||
---|---|---|---|---|---|---|
上调 Up-regulated | 下调 Down-regulated | 总计 Total | ||||
分子功能 Molecular function | 水解酶活性,作用于糖基键 Hydrolase activity, acting on glycosyl bonds | GO∶0016798 | 6 | 31 | 37 | 0.000 25 |
水解酶活性,水解O糖基化合物 Hydrolase activity, hydrolyzing O-glycosyl compounds | GO∶0004553 | 5 | 29 | 34 | 0.000 37 | |
氧化还原酶活性 Oxidoreductase activity | GO∶0016705 | 11 | 18 | 29 | 0.025 41 | |
铁离子结合 Iron ion binding | GO∶0005506 | 11 | 19 | 30 | 0.033 03 | |
糖跨膜转运蛋白活性 Sugar transmembrane transporter activity | GO∶0051119 | 6 | 4 | 10 | 0.036 38 | |
双加氧酶活性 Dioxygenase activity | GO∶0051213 | 6 | 9 | 15 | 0.044 21 | |
半乳糖苷酶活性Galactosidase activity | GO∶0015925 | 1 | 5 | 6 | 0.044 90 | |
生物过程 Biological process | 二糖生物合成过程 Disaccharide biosynthetic process | GO∶0046351 | 0 | 8 | 8 | 0.000 99 |
海藻糖生物合成过程 Trehalose biosynthetic process | GO∶0005992 | 0 | 7 | 7 | 0.002 67 | |
海藻糖代谢Trehalose metabolic process | GO∶0005991 | 0 | 7 | 7 | 0.003 73 | |
低聚糖生物合成过程 Oligosaccharide biosynthetic process | GO∶0009312 | 0 | 8 | 8 | 0.006 66 | |
激素代谢过程 Hormone metabolic process | GO∶0042445 | 2 | 11 | 13 | 0.011 38 | |
激素生物合成过程 Hormone biosynthetic process | GO∶0042446 | 2 | 2 | 4 | 0.032 92 | |
细胞组分 Cellular component | 细胞壁Cell wall | GO∶0005618 | 6 | 20 | 26 | 0.008 61 |
外部封装结构 External encapsulating structure | GO∶0030312 | 6 | 20 | 26 | 0.011 89 | |
植物型细胞壁Plant-type cell wall | GO∶0009505 | 2 | 9 | 11 | 0.358 56 |
Table 3 GO classification for the significant enrichment of differentially expressed genes
类别 Catergory | GO条目 GO term | 代谢节点编号 Metabolic node number | 差异表达基因数量 Number of DEGs | P值(校正后) P-value (corrected) | ||
---|---|---|---|---|---|---|
上调 Up-regulated | 下调 Down-regulated | 总计 Total | ||||
分子功能 Molecular function | 水解酶活性,作用于糖基键 Hydrolase activity, acting on glycosyl bonds | GO∶0016798 | 6 | 31 | 37 | 0.000 25 |
水解酶活性,水解O糖基化合物 Hydrolase activity, hydrolyzing O-glycosyl compounds | GO∶0004553 | 5 | 29 | 34 | 0.000 37 | |
氧化还原酶活性 Oxidoreductase activity | GO∶0016705 | 11 | 18 | 29 | 0.025 41 | |
铁离子结合 Iron ion binding | GO∶0005506 | 11 | 19 | 30 | 0.033 03 | |
糖跨膜转运蛋白活性 Sugar transmembrane transporter activity | GO∶0051119 | 6 | 4 | 10 | 0.036 38 | |
双加氧酶活性 Dioxygenase activity | GO∶0051213 | 6 | 9 | 15 | 0.044 21 | |
半乳糖苷酶活性Galactosidase activity | GO∶0015925 | 1 | 5 | 6 | 0.044 90 | |
生物过程 Biological process | 二糖生物合成过程 Disaccharide biosynthetic process | GO∶0046351 | 0 | 8 | 8 | 0.000 99 |
海藻糖生物合成过程 Trehalose biosynthetic process | GO∶0005992 | 0 | 7 | 7 | 0.002 67 | |
海藻糖代谢Trehalose metabolic process | GO∶0005991 | 0 | 7 | 7 | 0.003 73 | |
低聚糖生物合成过程 Oligosaccharide biosynthetic process | GO∶0009312 | 0 | 8 | 8 | 0.006 66 | |
激素代谢过程 Hormone metabolic process | GO∶0042445 | 2 | 11 | 13 | 0.011 38 | |
激素生物合成过程 Hormone biosynthetic process | GO∶0042446 | 2 | 2 | 4 | 0.032 92 | |
细胞组分 Cellular component | 细胞壁Cell wall | GO∶0005618 | 6 | 20 | 26 | 0.008 61 |
外部封装结构 External encapsulating structure | GO∶0030312 | 6 | 20 | 26 | 0.011 89 | |
植物型细胞壁Plant-type cell wall | GO∶0009505 | 2 | 9 | 11 | 0.358 56 |
Fig. 3 Contents of spike starch and soluble sugar after glyphosate ammonium treatmentNote: Different lowercase letters indicate significant differences between different groups of same variety at P<0.05 level.
Fig. 4 Contents of GA and IAA in grain ears after glyphosate ammonium salt treatmentNote: Different lowercase letters indicate significant differences between different groups of same variety at P<0.05 level.
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