Journal of Agricultural Science and Technology ›› 2025, Vol. 27 ›› Issue (1): 25-34.DOI: 10.13304/j.nykjdb.2023.0244

• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles     Next Articles

Research Progress and Application of Lactococcus lactis Expression System

Zhehan FENG(), Deping HUA, Xuefeng MEI, Baiping ZHAO, Lei ZHANG()   

  1. School of Life Sciences,Tianjin University,Tianjin 300027,China
  • Received:2023-03-30 Accepted:2023-08-25 Online:2025-01-15 Published:2025-01-21
  • Contact: Lei ZHANG

乳酸乳球菌表达系统的研究进展及其应用

冯哲瀚(), 华德平, 梅雪锋, 赵柏萍, 张蕾()   

  1. 天津大学生命科学学院,天津 300072

Abstract:

Lactococcus lactis is an important model strain in application and research of lactic acid bacteria in heterologous expression of proteins. L. lactis is an ideal host for heterologous protein expression due to its generally recognized as safe (GRAS) status, probiotic properties, the absence of inclusion bodies and endotoxins, surface display, and extracellular secretion. In view of the properties of L. lactis stimulating mucosal immunity, it has been widely used in presenting viruses and bacterial antigens.The recent research of L. lactis expression system was reviewed, with emphasis on the secretion and anchoring strategies of alloprotein expression of L. lactis, and the application of L. lactis as a microbial cell factory in food, medicine, especially antigen presentation were summarized. Finally, an outlook on the future research direction of the L. lactis expression system were provided.

Key words: Lactococcus lactis, expression system, heterologous proteins, secretion, surface display

摘要:

乳酸乳球菌是乳酸菌在异源表达蛋白方面的应用与研究中的一种重要模式菌,由于其公认的安全性、益生特性、无包涵体和内毒素、易于表面显示和细胞外分泌使其成为蛋白异源表达的理想宿主。由于乳酸乳球菌刺激黏膜免疫的特性,其在递呈病毒、细菌抗原等方面得到了广泛应用。阐述了乳酸乳球菌表达系统的相关研究进展,着重介绍了乳酸乳球菌表达异源蛋白的分泌和锚定策略,并总结了对乳酸乳球菌作为细胞工厂在食品、医药,特别是抗原呈递方面的应用,最后对乳酸乳球菌表达系统未来的研究方向进行了展望。

关键词: 乳酸乳球菌, 表达系统, 异源蛋白, 分泌表达, 表面展示

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