Generation of Maize Plants Transformed with ABP9 gene

doi:10.3969/j.issn.1008-0864.2010.06.18

›› 2010, Vol. 12 ›› Issue (6): 105-108.DOI: 10.3969/j.issn.1008-0864.2010.06.18

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Generation of Maize Plants Transformed with ABP9 gene

GAO Jian-qiang, ZHAO Fang, ZOU Wei-hua, LIANG Hua, ZHAO Jun

(Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China)

Received:2010-06-29 Revised:2010-08-16 Online:2010-12-15 Published:2010-12-13

玉米转ABP9基因植株的获得

高建强,赵芳,邹维华,梁华,赵军

(中国农业科学院生物技术研究所, 国家农作物基因资源与基因改良重大科学工程, 北京 100081)

通讯作者: 赵军,研究员,博士,研究方向为植物抗逆分子生物学和基因工程。Tel:010-82106139; E-mail:junzhao@caas.net.cn
作者简介:高建强,博士研究生,从事玉米抗逆转基因育种研究。Tel:010-82106137|E-mail:gaojq123@163.com。
基金资助:
国家转基因生物新品种培育重大专项(2008ZX08003-004)资助。

Abstract

Abstract:

ABP9 is involved in ABA signal transduction, control of ROS and enhancement of plant tolerance to abiotic stress. In order to obtain ABP9 transgenic maize plants to provide new material for stress-resistant breeding, a plant vector overexpressing transcription factor ABP9 driven by constitutive promoter Ubi was constructed and transformed into maize inbred Qi319 through Agrobacterium mediated transformation system. Transgenic maize plants were obtained by screening with herbicide glufosinate and PCR amplification of ABP9 with two different pairs of gene-specific primers.

Key words: maize, transgenic, stress resistance, ABP9

摘要：

ABP9参与ABA信号传导及ROS代谢调节,增强植物抵抗非生物逆境胁迫的能力,因此该基因在作物抗逆性改良上有潜在应用价值。为了获得转ABP9基因的玉米植株,向玉米抗逆育种提供新材料,构建了由组成型启动子Ubi驱动抗逆转录因子ABP9基因的植物表达载体,并通过农杆菌介导对玉米自交系齐319进行了遗传转化。经草铵膦抗性筛选和用两对不同的基因特异引物进行PCR扩增检测,证明已将ABP9基因导入玉米齐319基因组中,获得转基因植株。

关键词: 玉米;转基因;抗逆;ABP9

CLC Number:

S513
Q789

GAO Jian-qiang, ZHAO Fang, ZOU Wei-hua, LIANG Hua, ZHAO Jun. Generation of Maize Plants Transformed with ABP9 gene[J]. , 2010, 12(6): 105-108.

高建强,赵芳,邹维华,梁华,赵军. 玉米转ABP9基因植株的获得[J]. , 2010, 12(6): 105-108.

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Generation of Maize Plants Transformed with ABP9 gene

玉米转ABP9基因植株的获得

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