Abstract:

Two kinds of technology ： artificial low temperature and natural winter temperature, have been adopted for primary inducement culture of bulb scale segment of Lilium davidii vat. unicolor （Hoog） Cotton. The optimal culture medium for inducement is MS ＋ 0. 0～1.0 mg/L BA ＋ 0.0 ～ 0.1 mg/L NAA. It shows that the cold storage is dispensable, but not necessary for inducement of bulb. The culture condition of high temperature （28 - 29℃ ） , using clumpy buds for multiplication realizes the high frequency propagation of Lilium davidii vat. unicolor （Hoog） Cotton, with subculture medium of MS ＋0.0 ～ 0.5 mg/L BA ＋ 0.0 ～ 0.1 mg/L NAA. The rate of multiplication reaches 3.0  5.0. One multiplication period only needs 16 - 18 days. The field transportation of single bulb seedling from tissue culture helps to improve the rate of survival and reduce the amount of seedlings used in this process. This technique can be used for mass production of tissue culture seedlings and seedling transportation as well.

Key words: Lilium davidii var. unicolor （Hoog） Cotton, tissue culture, high frequency propagation

摘要：

采用人工低温及自然冬季萌芽两条路线进行兰州百合（L．davidiivat．unicolor（Hoog）Cotton）鳞片外植体初代诱导培养，以MS＋0．0～1．0mg／LBA＋0．0～0．1NAA为初代培养基，成功实现了南方高温地区兰州百合组织培养初代诱导。低温冷藏有利于鳞芽诱导，但不是鳞芽诱导的必要条件。采用MS＋0．0～0．5BA＋0．0～0．1NAA继代培养基，高温（28～29℃）培养与丛芽继代方式实现了兰州百合高频繁殖，其繁殖系数达3．0～5．0，继代周期为16～18d；组培生根苗的分单株大田移栽方法提高了成活率，降低了用苗量；为工厂化生产其组培苗及炼苗移栽提供了技术支持。

关键词: 兰州百合 组织培养 高频繁殖