Abstract:

Four types of enzymes were used to hydrolyze prepared soybean protein isolates and to study degree of hydrolysis and ACE （ angiotensin I-converting enzyme） inhibitory activity of the product. The results showed that alcalase was the optimum enzyme to produce soybean peptide. The optimum alcalase hydrolysis process for producing ACE inhibitory peptides was under 60 ℃ ,with pH 8.0, 4% substrate concentration,4% enzyme concentration, and 14.4% hydrolysis degree. After optimization, the ACE inhibitory activity can reach 84.1%.

Key words: soybean protein, angiotensin I converting enzyme inhibitory peptide, high performance liquid chromatograpy（HPLC） , enzymatic hydrolysis , degree of hydrolysis

摘要：

应用4种蛋白酶酶解大豆分离蛋白,研究其水解效果和降压活性。实验选定碱性蛋白酶为生产大豆降压肽的最适酶,并对其酶解条件进行了优化,确定生产大豆降压肽的最佳条件为：温度60℃,pH8.0,底物浓度4%,碱性蛋白酶浓度4%,水解度14.4%,优化后的ACE抑制率可达到84.1%。

关键词: 大豆蛋白 ACE抑制肽 高效液相色谱 酶解 水解度