Abstract:

A novel bio-sensing technology based on enzyme-catalyzed depositing enlargement was proposed to detect Turnip mosaic virus (TuMV). By using this method, the alkaline phosphatase-labeled antibody (TuMV antibody) was firstly immobilized on the electrode surface through sandwich immunity reaction, then the  infusible deposition was formed on the electrode surface by using alkaline phosphatase catalyze ascorbic acid to reduce silver ions. Thus the electrochemical signal of the electrode was enlarged greatly. The effects of experimental conditions, such as the concentration of antibody and the depositing time on the performances of immunoassay were studied. The result showed a fine linear relationship between reduction wave peak current and the concentration of TuMV from 10 to 1 000-fold dilution of TuMV primary solution with the detection limit of 500 000-fold dilution of TuMV primary solution.

Key words: TuMV, immunosensor, alkaline phosphatase, anodic stripping voltammetry

摘要：

提出了一种基于酶催化沉积放大检测芜菁花叶病毒(Turnip mosaic virus, TuMV)的生物传感技术。该方法先通过夹心免疫反应,将碱性磷酸酯酶标记的芜菁花叶病毒抗体固定到电极表面,然后通过碱性磷酸酯酶催化还原银离子在电极表面形成不溶性沉积物,从而放大电化学检测信号。考察了抗体的用量和沉积时间对免疫分析的影响,结果显示传感器信号响应与芜菁花叶病毒原液的浓度在10~1 000倍稀释范围内呈良好的线性关系,检出限达到500 000倍芜菁花叶病毒原液稀释浓度。

关键词: 芜菁花叶病毒；免疫传感器；碱性磷酸酯酶；阳极溶出伏安法