Determination of Adenosine in Bee Pollen by High Performance Liquid Chromatography

›› 2009, Vol. 11 ›› Issue (S1): 72-74.

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Determination of Adenosine in Bee Pollen by High Performance Liquid Chromatography

YUE Bing, XUE Xiao-feng, WU Li-ming, LI Yi, ZHAO Jing

(Institute of Apicultural Research, Chinese Academy of Agricultural Sciences|Bee Products Quality Supervision and Testing Center, Ministry of Agriculture|Apicultural Branch Center, Research and Development Center of National Agro-food Processing Technology, Beijing 100093, China)

Received:2009-08-15 Revised:2009-10-20 Online:2009-12-16 Published:2009-12-15

高效液相色谱法测定蜂花粉中的腺苷

岳兵,薛晓锋,吴黎明,李熠,赵静

(中国农业科学院蜜蜂研究所, 农业部蜂产品质量监督检验测试中心, 国家农产品加工技术研发中心蜂业分中心, 北京 100093)

通讯作者: 赵静,研究员,研究方向蜂产品质量与安全。E-mail:zhaojingjun@sina.com
作者简介:岳兵,硕士研究生,研究方向蜂产品质量与安全。E-mail:mooncake_333@126.com
基金资助:
国家蜜蜂产业技术体系建设专项经费;中央级公益性科研院所基本科研业务费专项(0032009015)资助。

Abstract

Abstract:

A high performance liquid chromatography (HPLC) method to determine adenosine in bee pollen was established. The adenosine in the sample was extracted by 10% ethanol, purified by Waters OASIS SPE cartridge and separated with reverse phase HPLC column and detected at 257 nm. The results show that the linearity is good (R2=0.999 9), the recovery is high(97%~101%) and precision is ideal. In conclusion, this method is simple, accurate and reliable.

Key words: bee pollen, adenosine, SPE, HPLC

摘要：

建立了一种测定蜂花粉中腺苷含量的高效液相色谱法。样品经10%乙醇提取,过 Waters OASIS柱固相萃取净化,液相色谱分离,257 nm波长处检测,腺苷在1~100 μg/mL范围内呈现良好的线性关系,R2=0.999 9,回收率较高,达到97%~101%,精密度好,相对标准偏差小于5％。该方法操作简单、准确、可靠。

关键词: 蜂花粉;腺苷;固相萃取;高效液相色谱

CLC Number:

S896.4

YUE Bing, XUE Xiao-feng, WU Li-ming, LI Yi, ZHAO Jing. Determination of Adenosine in Bee Pollen by High Performance Liquid Chromatography[J]. , 2009, 11(S1): 72-74.

岳兵,薛晓锋,吴黎明,李熠,赵静. 高效液相色谱法测定蜂花粉中的腺苷[J]. , 2009, 11(S1): 72-74.

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Determination of Adenosine in Bee Pollen by High Performance Liquid Chromatography

高效液相色谱法测定蜂花粉中的腺苷

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