Transcriptome Analysis of Watermelon Responses to Low Nitrogen Stress

doi:10.13304/j.nykjdb.2024.0156

Abstract

Abstract:

Nitrogen is one of the essential elements for plants， which is very important for the growth and development of watermelon. In order to elucidate the mechanism of watermelon responses to low nitrogen stress， the phenotypic changes of watermelon under continuous low nitrogen stress were analyzed， and transcriptome sequencing was performed on watermelon leaves treated with different nitrogen levels （CK， N1， N3） for 15 d using Illumina Novaseq platform. The results showed that the low nitrogen treatment promoted the underground root elongation of watermelon， but significantly inhibited the above ground plant growth. Transcriptome sequencing results showed that 121， 1 184 and 516 differentially expressed genes （DEGs） were identified in CK/N1， N1/N3 and CK/N3 comparisons， respectively. A total of 12 DEGs were identified in the 3 comparisons， among which the numbers of up-regulated and down-regulated genes were 1 and 11， respectively. The down-regulated genes responded to low nitrogen stress mainly through nicotinamide synthase， bHLH transcription factor， sugar transporter， chlorophyll metabolism and other pathways， while the up-regulated gene is related to tyrosine metabolism. In conclusion， the response of watermelon growth to low nitrogen stress was a complex process， and the key genes related to nitrogen stress identified by transcripome might play an important role in improving watermelon tolerance to low nitrogen stress and improving nitrogen use ef?ciency of watermelon. Above results laid a foundation for further research on the mechanism of watermelon response to low nitrogen stress.

Key words: watermelon, respond, low nitrogen stress, transcriptome analysis

摘要：

氮是植物必需的大量元素之一，对于西瓜生长发育至关重要。为阐明西瓜响应低氮胁迫的作用机制，对连续低氮胁迫下西瓜的表型变化进行分析，并利用Illumina Novaseq平台对3个氮素水平（CK、N1、N3）处理下第15天的西瓜叶片进行转录组测序。结果表明，低氮处理促进了西瓜地下根系伸长，但对地上植株长度和地上鲜重有明显抑制作用，处理后植株表现出地上部矮小的症状。转录组测序结果显示，在CK/N1、N1/N3、CK/N3对比中，分别鉴定出121、1 184、516个差异表达基因（differentially expressed genes，DEGs），其中共有的DEGs有12个，上调1个和下调11个，下调表达基因主要通过烟酰胺合成酶、bHLH转录因子、糖转运蛋白、叶绿素代谢等通路来响应低氮胁迫，而上调表达基因与酪氨酸代谢有关。综上，西瓜响应低氮胁迫是个复杂的过程，经转录组鉴定的与氮胁迫相关的关键基因在提高西瓜对低氮胁迫的耐受性以及提高西瓜的氮利用效率方面有重要作用。以上研究结果为深入研究西瓜低氮胁迫响应机理奠定了基础。

关键词: 西瓜, 响应, 低氮胁迫, 转录组分析

CLC Number:

S651

Zhan ZHAO, Xiaoting WANG, Lifeng ZHANG, Jinhe ZHAO, Yuhong YU, Junhua LI, Zhanqing WU. Transcriptome Analysis of Watermelon Responses to Low Nitrogen Stress[J]. Journal of Agricultural Science and Technology, 2024, 26(12): 30-38.

赵展, 王晓婷, 张黎凤, 赵津禾, 于玉红, 李军华, 吴占清. 西瓜对低氮胁迫响应的转录组分析[J]. 中国农业科技导报, 2024, 26(12): 30-38.

Figures/Tables 7

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样品 Sample	差异表达基因数量 Number of DEGs
样品 Sample	总计 Total	上调 Up-regulated	下调 Down-regulated
CK/N1	121	34	87
N1/N3	1 184	843	341
CK/N3	516	327	189

样品 Sample	差异表达基因数量 Number of DEGs
样品 Sample	总计 Total	上调 Up-regulated	下调 Down-regulated
CK/N1	121	34	87
N1/N3	1 184	843	341
CK/N3	516	327	189

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