Abstract:

In order to manufacture low cost, selenium-rich bifidobacterium preparation with effect of biostime, this paper took pumpkin as principal raw material, optimized the best culture medium formula through response surface methodology, and inspected a variety of bifidobacterium growth in optimul culture medium and selenium-rich ability. The result indicated that when the culture medium contained pumpkin juice 16.09%, soya peptone 3.24% and cysteine hydrochloride 0.28‰, the viable number of animal live Bifidobacterium 01 could reach 9.37±0.08 lgCFU/mL, which was higher than that cultured in medium MRS, GMRS, PYG and TPY. Cultured in the above mentioned optimal pumpkin juce medium, the contents of organic selenium in B. animalis, B. infantis, B.longum, B. adolescentis, B. bifidum, B. lactic and B. pseudocatenulatum were varied from 13.20±0.39~78.11±0.42 μg/g bacteria. The conversion rates of organic selenium were 1.76%~6.41%. The obtained Bifidobacterium taking pumpkin juice and skimmed milk as protective agent could achieve 70.8%±0.5% freeze-drying viability. The results proved that pumpkin was a promising substrate as cryoprotectant for Bifidobacterium spp. This study provided theoretical basis for manufacturing selenium-rich Bifidobacterium spp. pumpkin powder.

Key words: bifidobacterium, pumpkin, response surface, selenium enrichment

摘要：

为研制成本低且具有合生元效果的富硒双歧杆菌制剂，以南瓜为主要原料，通过响应面法优化最佳培养基配方，考察多种双歧杆菌在最优培养基中的生长情况及富硒能力。研究结果表明，当培养基组成为：南瓜汁浓度16.09%、大豆蛋白胨3.24%、半胱氨酸盐酸盐0.28‰时，动物双歧杆菌01的活菌数可以达到9.37±008 lgCFU/mL，高于在MRS、GMRS、PYG和TPY培养该菌所得的活菌数量。在上述优化南瓜汁培养基中培养，动物双歧杆菌01、婴儿双歧杆菌、长双歧杆菌、青春双歧杆菌、短双歧杆菌、假小链双歧杆菌和动物双歧杆菌BB12的有机硒含量可以达到(13.20±0.39)~(78.11±0.42) μg/g菌体，有机硒转化率为1.76%~6.41%。培养获得的双歧杆菌以南瓜汁和脱脂乳为保护剂，冻干存活率可以达到70.8%±0.5%。研究证明了南瓜果肉作为双歧杆菌增殖和保护基质的潜质，并为富硒双歧杆菌南瓜粉制剂的研制提供了理论依据。

关键词: 双歧杆菌；南瓜；响应面；富硒