Abstract: Orobanche aegyptiaca is a root parasitic plant, which has a serious impact on the yield of processed tomatoes. In order to reduce its harm, firstly, through the germ tube growth test in a petri dish, it was determined that Bacillus subtilis DNKAS had a significant inhibitory effect on the growth of germ tube of O. aegyptiaca; and by drip irrigation with water in field plot,  the control effect and yield-increasing effect of 8  treatments were  compared, including water (CK), 3 kg·hm^-2 DNKAS bacteria agent (D3), 4.5 kg·hm^-2 DNKAS bacteria agent (D4.5), 6 kg·hm^-2 DNKAS bacteria agent (D6), 9 kg·hm^-2 DNKAS bacteria agent (D9), 12 kg·hm^-2 DNKAS bacteria agent (D12), 7.5 kg·hm^-2 fulvic acid potassium (FA-K), 4.5 kg·hm^-2 DNKAS bacteria agent + 7.5 kg·hm^-2 fulvic acid potassium (D4.5+FA-K). Then, Biolog-ECO microplate detection method was used to analyze the changes of ecological and metabolic diversity of soil microbial community after microbial inoculum application, and to clarify the impact of microbial inoculum on crop and environment. The results showed that under the condition of pure bacteria application, the control effect of D12 treatment was the best, with the control effect  52.33%. The parasitic number and parasitic degree of O. aegyptiaca was the lowest, and the yield of tomato was 133 455 kg·hm^-2, with an increase of 56.48%. The effects of mixed application of potassium fulvate and bacteria agent was obviously superior to that of single application with potassium fulvate, the control effect was improved by 14%, the parasitism degree and parasitism number were decreased by about 10%, and the tomato yield was increased by 14%. The metabolic activity of microorganisms in different treatments was D4.5+FA-K > FA-K > D12> D9. There was no significant difference in the utilization rate of carbon sources among different treatments, but significant difference in Shannon diversity index, which indicated that the biocontrol bacteria DNKAS could increase the richness of soil community, and the application of biocontrol bacteria KNKAS and its combination with fulvic acid could improve the control effect, which was suitable for the control of O. aegyptiaca.

Key words: Bacillus subtilis, Orobanche aegyptiaca, control, processing tomato

摘要： 分枝列当（Orobanche aegyptiaca）是一种根寄生植物，严重影响加工番茄的产量。为减轻其危害，首先通过皿内芽管生长试验，明确了枯草芽孢杆菌DNKAS对分枝列当芽管生长有显著抑制作用；并通过田间小区随水滴灌方式，设置清水对照（CK）、3 kg·hm^-2 DNKAS菌剂（D3）、4.5 kg·hm^-2 DNKAS菌剂（D4.5）、6 kg·hm^-2 DNKAS菌剂（D6）、9 kg·hm^-2 DNKAS菌剂（D9）、12 kg·hm^-2 DNKAS菌剂（D12）、7.5 kg·hm^-2黄腐酸钾（FA-K）、4.5 kg·hm^-2 DNKAS菌剂+7.5 kg·hm^-2黄腐酸钾(D4.5+FA-K) 共8个处理，研究不同处理对分枝列当的防治效果和增产效果；随后采用 Biolog-ECO微平板检测法，分析菌剂施用后土壤微生物群落的生态及代谢多样性变化，明确菌剂对作物及环境的影响。结果表明，纯菌剂施用条件下，D12处理的防治效果最佳，防效为52.33%，列当寄生数量最低为493个，寄生度为1.30，番茄产量为133 455 kg·hm^-2，增产56.48%。黄腐酸钾与菌剂混合施用效果明显优异于其单独施用，防治效果提高了14%，寄生度和寄生数降低约10%，番茄产量提高14%。不同处理的微生物代谢活性表现为：D4.5+FA-K > FA-K > D12> D9，不同处理间的碳源利用率差异不明显，Shannon多样性指数差异显著，表明生防菌DNKAS可增加土壤群落丰富度，生防菌KNKAS及其与黄腐酸复配可提高防效，适用于分枝列当防治。

关键词: 生防菌, 分枝列当, 防治, 加工番茄