关键词: 杂交兰, 根状茎, 组织培养, 增殖, 分化

Abstract: Taking rhizome formed by crossing Cymbidium hybridum and Zhushalan as material, this experiment was carried out using KT, 6-BA and NAA 3 different exogenous hormones on the hybrid orchidrhizomes to compare the effects of different hormones on the proliferation and differentiation of rhizomes. In single factor experiment, along with the increase of hormone KT and 6-BA concentration, the proliferation and differentiation rates of hybrid orchid rhizomes were increased gradually, but not a directly proportional relation was formed. When NAA concentration was constant, 6-BA concentration reached 3 mg/L, the average proliferation rate of hybrid orchid rhizomes was up to 290%. When 6-BA concentration was 4 mg/L, its average differentiation rate was up to 85.64%. When KT concentration was 2.5 mg/L, the average proliferation rate was 300%, and KT concentration was 3.0 mg/L, its average differentiation rate was up to 87.89%. The combined addition of 3 hormones had the best effect on the proliferation and differentiation of hybrid orchid rhizomes. The best proliferation medium was 1/2 MS + 6-BA 3.0 mg/L + KT 2.0 mg/L + NAA 0.2 mg/L + agar 7.0 g/L + banana 80 g/L + sucrose 30 g/L + activated carbon 1 g/L. The rhizome thickness and length were larger and longer, and proliferation rate was higher. The best medium for differentiation effect was 1/2 MS + 6-BA 4.0mg/L + KT 2.5 mg/L + NAA 0.2mg/L + agar 7.0 g/L + banana 80 g/L + sucrose 30 g/L + activated carbon 1 g/L. When the differentiation seedlings grew well with dark green leaves. This study has laid certain theoretical basis for industrialization of breeding superior hybrid orchid strains.

Key words: hybrid cymbidium, rhizome, tissue culture, proliferation, differentiation