关键词: Biolog-ECO, 土壤调理剂, 碳代谢指纹, 植烟土壤

Abstract: In order to explore the effects of soil conditioner on soil microbial functional diversity in tobacco planting fields, this study took flue-cured tobacco variety Yunyan 87 as material to explore the differences of microbial carbon metabolism fingerprints in tobacco planting soil by Biolog-Eco detection method under 4 different levels of soil conditioner including CK(0 kg/hm2), T1(1 500 kg/hm2), T2(3 000 kg/hm2), T3（4 500 kg/hm2）. The results showed that: ① When the application amount of soil conditioner was 3 000 kg/hm2, flue-cured tobacco had the strongest ability to use the 4 kinds of carbon sources, including carboxylic acids, amines, polymers and other types during tobacco maturing period. ② Principal component analysis showed that there were 4 kinds of sensitive carbon sources to distinguish soil conditioner, including carbohydrate, carboxylic acids, amino acids and amines; while sensitive carbon sources polymers and other types were to distinguish soil conditioner application amount. ③ Soil microbial community functional diversity index showed that T2 had the highest of mcIntosh index, 82.85% higher than that of the CK. Moreover, there were no significant difference in Richness index and Simpson index among the 4 treatments. Thus, it could be seen that the application of soil conditioner had significantly improved carbon metabolism intensity and community functional diversity of the tobacco planting soil. 3 000 kg/hm2 application rate had the best performance. The above results provided certain theoretical basis for applying soil conditioner in tobacco planting fields.

Key words: Biolog-ECO, soil conditioner, carbon metabolism fingerprint, tobacco planting soil