中国农业科技导报 ›› 2021, Vol. 23 ›› Issue (12): 66-75.DOI: 10.13304/j.nykjdb.2020.0618

• 生物技术 生命科学 • 上一篇    下一篇

甘草PRR基因家族鉴定及表达分析

关思静1,王楠2,徐蓉蓉1,葛甜甜1,高静1*,颜永刚1,张岗1,陈莹1,张明英1   

  1. 1.陕西中医药大学药学院,陕西省秦岭中草药应用开发工程技术研究中心,西安712046;
    2.陕西中医药大学,陕西中药资源产业化省部共建协同创新中心,陕西 咸阳 712083
  • 收稿日期:2020-07-14 接受日期:2020-10-16 出版日期:2021-12-15 发布日期:2021-12-22
  • 通讯作者: 高静E-mail: gaojing@sntcm.edu.cn
  • 作者简介:关思静E-mail:gsj127810@163.com
  • 基金资助:

    国家自然科学基金项目(31600320);陕西省社会发展科技攻关项目(2018SF-338);

    陕西省高校科协人才托举项目(20170208);

    陕西中医药大学“秦药”品质评价及资源开发学科创新团队项目(2019-QN01)

GUAN Sijing1, WANG Nan2, XU Rongrong1, GE Tiantian1, GAO Jing1*, YAN Yonggang1, ZHANG Gang1, CHEN Ying1, ZHANG Mingying1#br#   

  1. 1.Shaanxi Qinling Chinese Herbal Medicine Application Development Engineering Technology Research Center, College of Pharmacy, Shaanxi University of Chinese Medicine, Xi’an 712046,China;
    2.Co-constrion Collabprative Innovation Center for Chinese Medicine Resources Industria Lization by Shaanxi & Education Ministry, 
    Shaanxi University of Chinese Medicine, Shaanxi Xianyang 712083, China
  • Received:2020-07-14 Accepted:2020-10-16 Online:2021-12-15 Published:2021-12-22

摘要: 伪应答调控(PRR)蛋白作为植物生物钟中央振荡器的重要组成部分,参与植物昼夜节律的调节,在植物生长发育、逆境胁迫响应等生命活动中发挥重要作用。利用生物信息学方法鉴定了甘草PRR蛋白编码基因,并结合转录组数据分析其在盐胁迫、干旱胁迫和低磷胁迫下的表达模式。结果表明:甘草基因组中共鉴定出7个GuPRR基因;根据系统发育进化分析将甘草PRR蛋白分为3个分支。在GuPRR启动子区还发现了大量的低温、干旱、光、茉莉酸甲酯、脱落酸和水杨酸响应的顺式元件。转录组数据分析结果表明,7个GuPRR基因在不同的组织都存在表达且响应不同的非生物胁迫。这些结果为甘草PRR基因家族的研究提供了理论的基础资料,将有助于深入了解甘草PRR基因家族的功能。

关键词: 甘草, PRR基因家族, 生物信息学分析, 基因表达

Abstract: The pseudo-response regulator (PRR) proteins  are important part of the central oscillator of plant biological clocks. PRRs participate in the regulation of plant circadian rhythm. In addition, PRRs also play important roles in growth, development and stress adaptation. In this study, the PRR family members were identified in licorice (Glycyrrhiza uralensis Fisch.) through bioinformatics methods, and its expression patterns were analyzed under salt, low phosphorus and drought stresses in virtue of transcriptome data. The results showed that: a total of seven GurPRR members were identified in the licorice plant genome and divided into three clades based on the phylogenetic results. Moreover, numerous cold-, drought-, light-, methyl jasmonate-, abscisic acid- and salicylic acid-responsive cis-elements were observed in the GuPRR promoter regions. Transcriptome data showed that the expressions patterns of PRR family genes in Glycyrrhiza uralensis were different in different tissues and responded to different stresses. The results provide abundant information about PRR family genes in Glycyrrhiza uralensis that would benefit for  further understanding of functional roles in licorice plants.

Key words: Glycyrrhiza uralensis, PRR gene family, bioinformatics analysis, gene expression