有机肥和钙肥对盐碱土花生根际细菌群落结构的影响

doi:10.13304/j.nykjdb.2021.0119

摘要/Abstract

摘要：

为研究有机肥和钙肥对盐碱地花生根际微生物群落结构和功能的影响，以不施肥为对照（CK）、设置施用钙肥（C）、有机肥（M）和有机肥+钙肥（CM）处理，通过构建细菌16S rRNA基因文库和高通量测序技术对不同处理下花生根际微生物进行测序。结果表明，施用有机肥、钙肥和有机肥钙肥配施均显著影响较轻度盐碱土壤花生根际微生物的多样性和丰富度，而对较重度盐碱土花生根际细菌的多样性和丰富度无显著影响。PCoA分析表明，样本OTUs多样性与土壤盐碱程度关系密切，施用钙肥处理花生根际微生物的菌群结构与其他处理间存在较大差异。两种滨海盐碱土花生根际微生物的种群结构均受施用有机肥、钙肥及其配施的影响，不同处理根际微生物均具有相同的优势菌群，但各优势菌群的相对丰度存在较大差异。在目和科水平上，有占总细菌76.3%～82.5%的新种OTUs未能注释，说明黄河三角洲滨海盐碱土花生根际蕴含大量微生物新种资源。16S rRNA功能预测分析表明，施用钙肥和有机肥处理均可显著提高2种土壤花生根际微生物碳水化合物代谢、氨基酸代谢、能量代谢、辅助因子和维生素的代谢、核苷酸代谢、翻译和膜运输等相关功能基因丰度。由此可见，施用钙肥和有机肥有利于改良盐碱土壤根际微生态环境，提高植物胁迫耐受性。

关键词: 盐碱土, 花生根际, 微生物菌群多样性, 有机肥, 钙肥, 16S rRNA

Abstract:

To Study the effects of organic fertilizer and calcium fertilizer on the microbial community structure and function in the peanut rhizosphere of saline-alkali soils， the peanut rhizosphere soils with different salinity contents （slightly and severely saline-alkali soils） were performed experiments with control （no fertilization， CK）， calcium fertilizer （C）， organic fertilizer （M） and organic fertilizer+calcium fertilizer （CM）. Then the peanut rhizosphere soils were used to extract DNA for constructing bacterial 16S rRNA gene library， and high-throughput sequencing was performed for sequencing and bioinformatics analysis. The results showed that the application of organic fertilizer， calcium fertilizer and the combination of organic fertilizer and calcium fertilizer had significant effects on the diversity and richness of rhizosphere microbes in slightly saline-alkali soil， but had no significant effects in severely saline-alkali soil. PCoA analysis showed that the OTUs diversity was closely related to the salinity contents of soil， and the structure of the rhizosphere microbial community treated with calcium fertilizer was significantly different from those of other treatments. The rhizosphere microbial structures of the two kinds of coastal saline soils were affected by organic fertilizer， calcium fertilizer and the combination of organic fertilizer and calcium fertilizer. The dominant bacterial communities of different treatments were similar， while the relative abundances of dominant bacterial communities were difference in different treatments. On the order and family levels， OTUs of new species accounted for 76.3%~82.5% of the total bacteria， which indicated the rhizosphere in the coastal saline soil of the Yellow River Delta contained a large number of new microbial species resources. The analysis of 16S rRNA function prediction indicated that the application of organic fertilizer， calcium fertilizer and the combination of organic fertilizer and calcium fertilizer increased the abundances of genes on carbohydrate transport and metabolism， amino acid metabolism， energy production and conversion， metabolism of cofactors and vitamins， nucleotide metabolism， translation and membrane transport. In conclusion， the application of calcium fertilizer and organic fertilizer could be beneficial to improve the rhizosphere microecological environment of saline-alkali soil and enhance plant stress tolerance.

Key words: saline-alkali soil, peanut rhizosphere, microbial community diversity, organic fertilizer, calcium fertilizer, 16S rRNA

中图分类号:

S565.2

戴良香, 张冠初, 丁红, 徐扬, 张智猛. 有机肥和钙肥对盐碱土花生根际细菌群落结构的影响[J]. 中国农业科技导报, 2022, 24(5): 189-201.

Liangxiang DAI, Guanchu ZHANG, Hong DING, Yang XU, Zhimeng ZHANG. Effects of Organic Fertilizer and Calcium Fertilizer on Peanut Rhizosphere Bacterial Community Structure in Saline-alkali Soil[J]. Journal of Agricultural Science and Technology, 2022, 24(5): 189-201.

图/表 10

表1 供试土壤样品的理化性状

Table 1 Characteristics of soil samples for test

采样地点

Sampling site

含盐量

Salt content/（g·kg^-1）

有机质含量

Organic matter/ （g·kg^-1）

全氮

Total nitrogen/（g·kg^-1）

碱解氮

Available nitrogen/

（mg·kg^-1）

速效磷

Available phosphorus/（mg·kg^-1）

速效钾

Available potasium/

（mg·kg^-1）

利津毛坨

Maotuo of Lijin County

垦利青坨

Qingtuo of Kenli County

图1 花生根际土壤微生物群落测序数据A：序列长度分布；B： OTU数量维恩图

Fig. 1 Overall sequence data of bacterial communities in the peanut rhizosphereA： Length distribution of trimmed sequences； B： Venn diagram of OTUs

表2 不同处理下根际土壤微生物的Alpha多样性指数

Table 2 Alpha diversity index of rhizosphere soil samples in each treatment

处理 Treatment	丰富度 Community richness			多样性 Community diversity		覆盖度 Coverage
处理 Treatment	Sobs	Chao	Ace	Shannon	Simpson	覆盖度 Coverage
CK1	3 216.5	3 508.856	3 459.115	7.253 0	0.001 34	0.991 11
C1	3 271.0	3 513.107	3 453.484	6.792 7	0.005 44	0.995 60
M1	3 400.5	3 626.337	3 578.135	7.247 3	0.001 50	0.993 79
MC1	3 211.5	3 484.869	3 442.857	7.171 5	0.001 62	0.991 75
CK2	3 142.5	3 466.744	3 414.230	7.120 0	0.001 84	0.989 75
C2	3 292.0	3 585.756	3 524.607	6.996 1	0.003 06	0.993 03
M2	3 163.5	3 405.369	3 350.942	6.822 0	0.003 65	0.995 00
MC2	3 227.5	3 497.223	3 448.287	6.745 6	0.006 47	0.993 16

图2 不同处理花生根际土壤微生物的多样性指数注：*和**分别表示不同处理间差异在P<0.05和P<0.01水平显著。

Fig. 2 Diversity index of rhizosphere soil microorganism in different treatmentsNote： * and ** indicate significant differences among treatments at P<0.05 and P<0.01 levels， respectively.

图3 Alpha多样性分析

Fig. 3 Alpha diversity analysis

图4 花生根际细菌群落Beta多样性分析

Fig.4 Beta diversity analysis of bacterial communities in the peanut rhizosphere

图5 门和纲水平各样本的菌落结构

Fig. 5 Bacterial community at the phylum and class levels in different treatments.

图6 目和科水平各样本细菌的群落结构

Fig. 6 Bacterial community at the order and famliy levels in different treatments.

图7 属和种水平各样本细菌群落结构

Fig. 7 Bacterial community at the genus and species levels in different treatments.

图8 土壤细菌菌群功能预测

Fig. 8 The bacterial functional features analysis.

参考文献 52

1	贾敬敦,张富.依靠科技创新推进我国盐碱地资源可持续利用[J].中国农业科技导报,2014,16(5):1-7.
	JIA J D, ZHANG F. Sustainable utilization of saline-alkali land resources through scientific and technological innovation in China [J]. J. Agric. Sci. Technol., 2014, 16(5):1-7.
2	张智猛,慈敦伟,丁红,等.花生品种耐盐性指标筛选与综合评价[J].应用生态学报,2013,24(12):3487-3494.
	ZHANG Z M, CI D W, DING H, et al.. Indices selection and comprehensive evaluation of salinity tolerance for peanut varieties [J]. Chin. J. Appl. Ecol., 2013, 24(12):3487-3494.
3	慈敦伟,张智猛,丁红,等.花生苗期耐盐性评价及耐盐指标筛选[J].生态学报,2015,35(3):805-814.
	CI D W, ZHANG Z M, DING H,et al..Evaluation and selection indices of salinity tolerance in peanut seedling [J]. Acta Ecol. Sin., 2015, 35(3):805-814.
4	张旭龙,马淼,吴振振,等.不同油葵品种对盐碱地根际土壤酶活性及微生物群落功能多样性的影响[J].生态学报,2017, 37(5):1659-1666.
	ZHANG X L, MA M, WU Z Z, et al..Effects of Helianthus annuus varieties on rhizosphere soil enzyme activities and microbial community functional diversity of saline-alkali land in Xinjiang [J]. Acta Ecol. Sin., 2017, 37(5):1659-1666.
5	ZHANG Q, YU X. Allelopathy in replant problem in forest soil [J]. Allelopathy J., 2001, 8:51-64.
6	KUZYAKOV Y, RAZAVI B S. Rhizosphere size and shape: temporal dynamics and spatial stationarity [J]. Soil Biol. Biochem., 2019, 135:343-360.
7	CORREA-GALEOTE D, BEDMAR E J, FERNÁNDEZ-GONZÁLEZ A J, et al.. Bacterial communities in the rhizosphere of amilaceous maize (Zea mays L.) as assessed by pyrosequencing [J/OL]. Frontiers Plant Sci., 2016, 7:1016 [2020-12-20]. .
8	SINGH A, SARMA B K, UPADHYAY R S, et al.. Compatible rhizosphere microbes mediated alleviation of biotic stress in chickpea through enhanced antioxidant and phenylpropanoid activities [J]. Microbiol. Res., 2013, 168(1):33-40.
9	HAW-KINS H J, LEW IS O A M. Effect of NaCl salinity, nitrogen form, calcium and potassium concentration on nitrogen uptake and kinetics in Triticum aestivum L. cv. gamtoos [J]. New Phytol., 1993,124:171-177.
10	CAINES A M, SHENNAN C. Interactive effects of Ca²⁺ and NaCl salinity on the growth of two tomato genotypes differing in Ca²⁺ use efficiency [J]. Plant Physiol. Biochem., 1999, 37:569-576.
11	李银鹏,林鹏.盐度对木榄幼苗某些金属元素累积的影响及钙的效应[J].应用生态学报,2000,11(2):177-180.
	LI Y P, LIN P. Impact of salinity on accumulation of several metal elements in Bruguiera gymnorrhiza seedlings and Ca effect [J]. Chin. J. Appl. Ecol., 2000, 11(2):177-180.
12	EHRHARDT D W, WAIS R, LONG S R. Calcium spiking in plant root hairs responding to Rhizobium nodulation signals [J]. Cell, 1996, 85:673-681.
13	CHIEN C T, SHETTY K, MORTIMER M, et al.. Calcium-induced salt tolerance in Rhizobium leguminosarum biovarviciae strain C124b [J]. FEMS Microbiol. Lett., 1991, 83:219-224.
14	孟磊,丁维新,蔡祖聪.长期定量施肥对土壤有机碳储量和土壤呼吸影响[J].地球科学进展,2005,20(6):687-692.
	MENG L, DING W X, CAI Z C, et al.. Storage of soil organic C and soil respirationas effected by long-term quantitative fertilization [J]. Adv. Earth Sci., 2005, 20(6):687-692.
15	WANG X L, REN Y Y, ZHANG S Q, et al.. Applications of organic manure increased maize (Zea mays L.) yield and water productivity in a semi-arid region [J]. Agric. Water Manage., 2017, 187:88-98.
16	孙薇,钱勋,付青霞,等.生物有机肥对秦巴山区核桃园土壤微生物群落和酶活性的影响[J].植物营养与肥料学报,2013,19(5):1224-1233.
	SUN W, QIAN X, FU Q X, et al.. Effects of bio-organic fertilizer on soil microbial community and enzymes activities in walnut orchards of the Qinling-Bashan Region [J]. J. Plant Nutr. Fert., 2013, 19(5):1224-1233.
17	范丙全.我国生物肥料研究与应用进展[J].植物营养与肥料学报,2017,23(6):1602-1613.
	FAN B Q. Advances in biofertilizer research and development in China [J]. J. Plant Nutr. Fert., 2017, 23(6):1602-1613.
18	LIU B, TU C, HU S, et al.. Effect of organic, sustainable, and conventional management strategies in grower fields on soil physical, chemical, and biological factors and the incidence of southern blight [J]. Appl. Soil Ecol., 2007, 37(3):202-214.
19	WEI S, TETSUO T, LIU S K. Isolation and characterization of an alkaliphilic and halotolerant Nesterenkonia. sp from an extreme soda saline-alkali soil in the northeastern of China [J]. Mol. Soil Biol., 2011, 64(11):914-920.
20	CUI C, MA L, SHI J, et al.. Metabolic pathway for degradation of nthracene by halophilic Martelella sp. AD-3 [J]. Int. Biodeterior. Biodegrad., 2014, 89(2):67-73.
21	李凤霞,郭永忠,许兴.盐碱地土壤微生物生态特征研究进展[J].安徽农业科学,2011,39(23):14065-14067, 14174.
	LI F X, GUO Y Z, XU X. Research progress of the microbial characteristics of saline-alkali soil [J]. J. Anhui Agric. Sci., 2011, 39(23):14065-14067, 14174.
22	李瀚,杨吉顺,张冠初,等.花生品种萌发期耐盐性比较鉴定[J].花生学报,2015,44(4):48-52.
	LI H, YANG J S, ZHANG G C, et al.. Identification of salt tolerancein germination period of peanut varieties [J]. J. Peant Sci., 2015, 44(4):48-52.
23	慈敦伟,戴良香,宋文武,等.花生萌发至苗期耐盐胁迫的基因型差异[J].植物生态学报,2013, 37(11):1018-1027.
	CI D W, DAI L X, SONG W W, et al.. Genotypic differences in salt tolerance from germination to seedling stage in peanut [J]. Chin. J. Plant Ecol., 2013, 37(11):1018-1027.
24	温赛群.苗期花生品种耐盐性鉴定及生理生化评价[D].河北保定:河北农业大学,2019.
	WEN S Q. Identification of salt tolerance of peanut germplasm and physiological evaluation [D]. Hebei Baoding: Hebei Agricultural University, 2019.
25	田家明,张智猛,戴良香,等.外源钙对盐碱土与非盐碱土花生生长发育与光合特性的影响[J].华北农学报,2018,33(6):130-136.
	TIAN J M, ZHANG Z M, DAI L X, et al.. Effects of exogenous calcium on the development and photosynthetic characteristics of peanut in saline-alkali soil and normal soil [J]. Acta Agric. Boreali-Sin., 2018, 33(6):130 -136.
26	张冠初,史晓龙,慈敦伟,等.干旱和盐胁迫对花生干物质积累及光合特性的影响[J].核农学报,2019,33(5):999-1005.
	ZHANG G C, SHI X L, CI D W, et al.. Effect of drought and salt stress on accumulation of plant dry weight and photosynthetic characteristics [J]. J. Nucl. Agric. Sci., 2019, 33(5):999-1005.
27	张冠初,张智猛,慈敦伟,等.干旱和盐胁迫对花生渗透调节和抗氧化酶活性的影响[J].华北农学报,2018,33(3):176-181.
	ZHANG G C, ZHANG Z M, CI D W, et al.. Effects of drought and salt stress on osmotic regulator and antioxidase activities [J]. Acta Agric. Boreali-Sin., 2018, 33(3):176-181.
28	史晓龙,张智猛,戴良香,等.外源施钙对盐胁迫下花生营养元素吸收与分配的影响[J].应用生态报,2018,29(10):3302-3310.
	SHI X L, ZHANG Z M, DAI L X, et al.. Effects of calcium fertilizer application on absorption and distribution of nutrients in peanut under salt stress [J]. Chin. J. Appl. Ecol., 2018, 29(10):3302-3310.
29	DAI L X, ZHANG G C, YU Z P, et al.. Effect of drought stress and developmental stages on microbial community structure and diversity in peanut rhizosphere soil [J/OL]. Int. J. Mol. Sci., 2019, 20(9):2265 [2020-12-20]. .
30	孙波,赵其国,张桃林,等.土壤质量与持续环境 Ⅲ:土壤质量评价的生物学指标[J].土壤,1997,29(5):225-234.
	SUN B, ZHAO Q G, ZANG T L, et al.. Soil quality and sustainable environment Ⅲ: biological indicators of soil quality evaluation [J]. Soils, 1997, 29(5):225-234.
31	STENBERG B. Monitoring soil quality of arable land: microbiological indicators [J]. Acta Agric. Scandinavica, 1999, 49(1):1-24.
32	WINDING A, HUND-RINKE K, RUTGERS M. The use of microorganisms in ecological soil classification and assessment concepts [J]. Ecotoxicol. Environ. Safety, 2005, 62(2):230-248.
33	张瑜斌,林鹏,魏小勇,等.盐度对稀释平板法研究红树林区土壤微生物数量的影响[J].生态学报,2008,28:1288-1296.
	ZHANG Y B, LIN P, WEI X Y, et al.. Effect of salinity on microbial densities of soil in the dilution plate technique applied in mangrove areas [J]. Acta Ecol. Sin., 2008, 28:1288-1296.
34	KHAN K S, GATTINGER A, BUEGGER F, et al.. Microbial use of organic amendments in saline soils monitored by changes in the 13C/12C ratio [J]. Soil Biol. Biochem., 2008, 40(5):1217-1224.
35	乔正良,来航线,强郁荣,等.陕西主要盐碱土中微生物生态初步研究[J].西北农业学报,2006,15(3):60-64.
	QIAO Z L, LAI H X, QIANG Y R, et al.. Primary study on microorganism ecology in saline-alkali soil of Shaanxi Province [J]. Acta Agric. Bor-Occid. Sin., 2006, 15(3):60-64.
36	李新,焦燕,杨铭德.用磷脂脂肪酸(PLFA)谱图技术分析内蒙古河套灌区不同盐碱程度土壤微生物群落多样性[J].生态科学,2014,33(3):488-494.
	LI X, JIAO Y, YANG M D. Microbial diversity of different saline-alkaline soil analyzing by PLFA in the Hetao area of Inner Mongolia [J]. Ecol. Sci., 2014, 33(3):488-494.
37	牛世全,景彩虹,廖世齐,等.河西走廊盐碱土细菌种群结构多样性的研究[J].西北师范大学学报(自然科学版),2013,49(2):90-95.
	NIU S Q, JING C H, LIAO S Q, et al.. Bacterial population structure diversity in saline-alkali soil in Hexi Corridor [J]. J. Northwest Normal Univ. (Nat. Sci.), 2013, 49(2):90-95.
38	黄明勇,杨剑芳,王怀锋,等.天津滨海盐碱土地区城市绿地土壤微生物特性研究[J].土壤通报,2007,38(6):1131-1135.
	HUANG M Y, YANG J F, WANG H F, et al.. Microbial properties in urban soils in coastal alkali-saline area of Tianjin [J]. Chin. J. Soil Sci., 2007, 38(6):1131-1135.
39	朱泓,王小敏,黄涛,等.NaCl 胁迫对滨梅根际细菌群落多样性及优势菌群的影响[J].南京林业大学学报(自然科学版),2017,40(4):49-54.
	ZHU H, WANG X M, HUANG T, et al.. Effect of NaCl stress on bacterial community diversity and core microbiome in rhizosphere and bulk soil of beach plum (Prunus maritima Marshall) [J]. J. Nanjing For. Univ. (Nat. Sci.), 2017, 40(4):49-54.
40	YANG Y, SHAO T Y, LONG X H, et al.. Microbiome structure and function in rhizosphere of Jerusalem artichoke grown in saline land [J/OL].Sci. Total Environ., 2020, 724(168):138259 [2020-12-20]. .
41	吉丽.盐碱胁迫下大豆根际微生物多样性分析[D].长春:吉林农业大学,2017.
	JI L. Diversity analysis of rhizosphere microorganisms in soybean under saline alkali stress [D]. Changchun: Jilin Agricultural University, 2017.
42	XIA J B, REN J Y, ZHANG S Y, et al.. Forest and grass composite patterns improve the soil quality in the coastal saline-alkali land of the Yellow River Delta, China [J]. Geoderma, 2019, 349:25-35.
43	SHI W, TAKANO T, LIU S K. Isolation and characterization of novel bacterial taxa from extreme alkali-saline soil [J].World J. Microbiol. Biotechnol., 2012, 28 (5):2147-2157.
44	VALENZUELA-ENCINAS C, NERIA-GONZ LEZ I, NTARA-HERN NDEZ R J, et al.. Changes in the bacterial populations of the highly alkaline saline soil of the former lake Texcoco (Mexico) following flooding [J]. Extremophiles, 2009, 13(4):609-621.
45	VALENZUELA-ENCINAS C, NERIA-GONZ LEZ I, NTARA-HERN NDEZ R J, et al.. Phylogenetic analysis of the archaeal community in an alkaline-saline soil of the former lake Texcoco (Mexico) [J]. Extremophiles, 2008, 12(2):247-254.
46	徐扬,张冠初,丁红,等.干旱与盐胁迫对花生根际土壤微生物群落结构的影响[J]].应用生态学报,2020,31(4):1305-1313.
	XU Y, ZHANG G C, DING H, et al.. Effects of salt and drought stresses on rhizosphere soil bacterial community structure and peanut yield [J]. Chin. J. Appl. Ecol., 2020, 31(4):1305-1313.
47	戴良香,康涛,慈敦伟,等.黄河三角洲盐碱地花生根层土壤菌群结构多样性研究[J].生态学报,2019,39(19):7169-7178.
	DAI L X, KANG T, CI D W, et al.. Comparison of the microbial community in the rhizosphere of peanuts between saline-alkali and non-saline soil at different soil depths and intercropping cultivation in the yellow river delta [J]. Acta Ecol. Sin., 2019, 39(19):7169-7178.
48	田平雅,沈聪,赵辉,等.银北盐碱区植物根际土壤酶活性及微生物群落特征[J].土壤学报,2020,57(1):217-226.
	TIAN P Y, SHEN C, ZHAO H, et al.. Enzyme activities and microbial communities in rhizospheres of plants in salinized soil in north Yinchuan, China [J]. Acta Pedolog. Sin., 2020, 57(1):217-226.
49	张美娟,王冰,黄升财,等.糠醛渣改良土壤增强苕子对盐碱土的适应性[J].农业工程学报,2020,36(6):115-121.
	ZHANG M J, WANG B, HUANG S C, et al.. Enhancing the adaptation of Vicia villosa Roth to salinity-alkalinity soils improved using furfural residues [J]. Trans. Chin. Soc. Agric. Eng., 2020, 36(6):115-121.
50	许来鹏,万鲜花,孙向丽,等.畜禽粪肥和秸秆还田对玉米根际微生物群落结构的影响[J].生物技术通报,2020,36(9):137-146.
	XU L P, WAN X H, SUN X L, et al.. Effects of livestock manure and straw returning to field on microbial community structure around maize rhizosphere [J]. Biotechnol. Bull., 2020, 36(9):137-146.
51	BAI Y C, MEI L J, ZUO W G, et al.. Response of bacterial communities in coastal mudflat saline soil to sewage sludge amendment [J]. Appl. Soil Ecol., 2019, 144:107-111.
52	俞冰倩.我国不同盐碱土生态系统土壤微生物群落多样性研究[D].江苏镇江:江苏大学,2019.
	YU B Q. Research on the diversity of microbial community in different saline-alkaline soil ecosystems in China [D]. Jiangsu Zhenjiang: Jiangsu University, 2019.

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