植物合成生物学领域发展态势分析

doi:10.13304/j.nykjdb.2022.0206

摘要/Abstract

摘要：

植物合成生物学为解决农作物育种及品质改良等重要农业问题带来新机遇。为了使相关科研和管理人员及时了解植物合成生物学的研究进展、把握领域科技前沿、有效开展科研合作、优化研究布局，从文献计量角度分析合成生物学的发展态势。基于Web of Science 核心集检索到2000—2020年研究文献97 551篇。计量分析结果表明，植物合成生物学领域论文呈现稳定增长趋势，中国学者发表的论文数量及总被引频次位居前列。通过对高被引文献以及文献主题聚类分析可知，重要作用元件如转录因子的调控机制、标准化的遗传元件如启动子、功能基因的挖掘和鉴定、植物天然产物合成与代谢通路的解析、基因编辑技术/多组学分析技术等关键技术是本领域的重要主题内容，将持续推动本领域的创新发展。此外，对比分析了国内外合成生物学的资助及研究布局，针对当前我国在植物合成生物学领域的资助现状提出展望，加强国家顶层设计，加大植物合成生物领域的资助。

关键词: 植物合成生物学, 文献计量, 关键技术, 态势分析, 研究布局

Abstract:

Plant synthetic biology brings new opportunities for solving important agricultural problems such as crop breeding and quality improvement. In order to enable researchers and administrators to timely understand of the research progress in plant synthetic biology， grasp the frontiers of science and technology in the field， effectively carry out scientific research cooperation， and optimize the layout of scientific research， this study analyzed the development trend of plant synthetic biology in China from the perspective of literature. Based on constructed retrieval strategy， 97 551 research papers （from 2000 to 2021） were searched from the Web of Science core set. The quantitative indexes reflected the quantity trend of papers published， the quantity and distribution characteristics of scientific research subjects， the content and evolution characteristics of literature topics. The results of indexes analysis showed that the number of papers published by Chinese scholars and the frequency of their citations were in the leading position in the world. According to the topic cluster analysis of the highly cited literature and the subject of the literature， it showed that the regulation mechanism of important functional elements such as transcription factors， the excavation and identification of standardized genetic elements such as promoters， functional genes， the analysis of the synthetic and metabolic pathways of natural plant products， key technologies such as gene editing and multi-group analysis were the important contents of this field， and would continue to drive innovation in this area. In addition， the funding and research layout of synthetic biology at home and abroad were compared and analyzed. Based on the current situation of funding in the field of plant synthetic biology in China， the prospect of strengthening national top-level design was put forward， with funding to be increased for plant synthetic biology.

Key words: plant synthetic biology, bibliometric, key technology, situation analysis, research layout

中图分类号:

Q812

李楠, 许哲平, 郭晓真, 王友华, 张学福. 植物合成生物学领域发展态势分析[J]. 中国农业科技导报, 2022, 24(9): 24-38.

Nan LI, Zheping XU, Xiaozhen GUO, Youhua WANG, Xuefu ZHANG. Development Trend in the Field of Plant Synthetic Biology[J]. Journal of Agricultural Science and Technology, 2022, 24(9): 24-38.

图/表 10

参考文献 48

1	ROELL M S, ZURBRIGGEN M D. The impact of synthetic biology for future agriculture and nutrition [J]. Curr. Opin. Biotechnol., 2020,61: 102-109.
2	王凯悦,陈芳泉,黄五星. 植物干旱胁迫响应机制研究进展[J].中国农业科技导报, 2019,21(2):19-25.
	WANG K Y, CHEN F Q, HUANG W X. Research advance on drought stress response mechanism in plants [J]. J. Agric. Sci. Technol., 2019, 21(2) :19-25.
3	马小倩,杨涛,张全,等.水稻新型育种技术研究现状与展望[J].中国农业科技导报, 2022,24(1):24-30.
	MA X Q, YANG T, ZHANG Q,et al.. Development status and prospect of rice new breeding [J]. J. Agric. Sci. Technol., 2022,24(1):24-30.
4	张先恩.中国合成生物学发展回顾与展望[J].中国科学:生命科学,2019,49(12):1543-1572.
	ZHANG X E. Synthetic biology in China: review and prospects [J]. Sci. Sin. (Vitae), 2019,49(12):1543-1572.
5	邓子新.合成生物学趁最好时代,建物致知,建物致用[J]. 中国科学:生命科学,2019,31(4):323-324.
	DENG Z X. Synthetic biology takes advantage of the best times, builds knowledge, builds use [J]. Sci. Sin. (Vitae), 2019, 31(4):323-324.
6	刘立中, 白阳, 郑海, 等. 合成生物学在基础生命科学研究中的应用[J].生物工程学报,2017, 33(3): 315-323.
	LIU LZ, BAI Y, ZHENG H, et al.. Fundamental aspects of synthetic biology [J]. Chin. J. Biotech., 2017, 33(3): 315-323.
7	江会锋.合成生物技术助力可持续发展[J].生物技术通报,2020,36(4):1-2.
	JIANG H F. Synthetic biotechnology boosts sustainable development [J]. Biotech. Bull., 2020,36(4):1-2.
8	吴杰, 赵乔.合成生物学在现代农业中的应用与前景[J].植物生理学报,2020,56(11):2308-2316.
	WU J, ZHAO Q. The application and prospect of synthetic biology in future agriculture [J]. J. Plant Physiol., 2020, 56 (11): 2308-2316.
9	林敏.农业生物育种技术的发展历程及产业化对策[J].生物技术进展, 2021,11(4): 405-417.
	LIN M. The development course and industrialization countermeasure of agricultural biological breeding technology [J]. Curr. Biotech., 2021,11(4): 405-417.
10	ZHU Q, WANG B, TAN J, et al.. Plant synthetic metabolic engineering for enhancing crop nutritional quality [J/OL]. Plant Commun.,2020, 1: 100017 [2022-02-04]. .
11	SCHWILLE P. Bottom-up synthetic biology: engineering in a tinkerer’s world [J]. Science,2011, 333(6047):1252-1254.
12	LIU W, STEWART C N J. Plant synthetic biology [J]. Trends Plant Sci., 2015,20(5):309-317.
13	李楠,李晓曼,张学福.全球植物科学领域发展态势分析[J].中国农学通报, 2020,36(34):148-159.
	LI N, LI X M, ZHANG X F. Trend and topic analysis of global plant science research [J]. Chin. Agric. Sci. Bull., 2020,36(34):148-159.
14	黄家章,卢士军,姚远,等.基于文献计量的国际营养导向型农业研究进展可视化分析[J].中国农业科技导报, 2020, 22(9): 11-21.
	HUANG J Z, LU S J, YAO Y, et al.. Visualization analysis of research progress of international nutrition-sensitive agriculture based on bibliometrics [J]. J. Agric. Sci. Technol., 2020, 22(9): 11-21.
15	刘佳,魏佳奇,刘玉琴,等.基于专利分析和社会网络分析的基因编辑技术演化研究[J].生物技术通报,2021, 37(12): 274-284.
	LIU J, WEI J Q, LIU Y Q, et al.. Research on evolution of gene editing technology based on patent analysis and social network analysis [J]. Biotech. Bull., 2021, 37(12): 274-284.
16	邱均平.文献信息增长规律与应用[J].情报理论与实践, 2000(2):153-157.
	QIU J P. Law of literature information growth and its application [J]. ITA, 2000(2):153-157.
17	DUBOS C, STRACKE R, GROTEWOLD E, et al.. MYB transcription factors in Arabidopsis [J]. Trends Plant Sci., 2010,15(10):573-581.
18	SZABADOS L, SAVOURÉ A. Proline: a multifunctional amino acid [J]. Trends Plant Sci., 2010,15(2):89-97.
19	RAGAUSKAS A J, BECKHAM G T, BIDDY M J, et al.. Lignin valorization: improving lignin processing in the biorefinery [J/OL]. Science, 2014,344(6185):1246843 [2022-02-04]. .
20	VOGT T. Phenylpropanoid biosynthesis [J]. Mol. Plant, 2010,3(1):2-20.
21	WASTERNACK C, HAUSE B. Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany [J]. Ann. Bot., 2013,111(6):1021-1058.
22	ROBERT-SEILANIANTZ A, GRANT M, JONES J. Hormone crosstalk in plant disease and defense: more than just JASMONATE-SALICYLATE antagonism [J]. Ann. Rev. Phytopathol., 2011,49:317-343.
23	MILLER J C, TAN S, QIAO G, et al.. A TALE nuclease architecture for efficient genome editing [J]. Nat. Biotech., 2011, 29(2):143-148.
24	URNOV F D, REBAR E J, HOLMES M C, et al.. Genome editing with engineered zinc finger nucleases [J]. Nat. Rev. Genet., 2010,11(9):636-646.
25	CHALHOUB B, DENOEUD F, LIU S. Early allopolyploid evolution in the post-neolithic Brassica napus oilseed genome [J]. Science, 2014,345(6199): 950-953.
26	IRAVANI S. Green synthesis of metal nanoparticles using plants [J]. Green Chem., 2011,13:2638-2650.
27	XIE K, MINKENBERG B, YANG Y. Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system [J]. Proc. Natl. Acad. Sci. USA, 2015,112(11):3570-3575.
28	MA X. A Robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants [J]. Mol. Plant, 2015,8(8):1274-1284.
29	GAUDELLI N M, KOMOR A C, REES H A, et al.. Programmable base editing of A.T to G.C in genomic DNA without DNA cleavage [J]. Nature, 2017,551(7681):464-471.
30	AHMED S, AHMAD M, SWAMI B L, et al.. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise [J]. J. Adv. Res., 2016,7(1):17-28.
31	BENELLI G. Plant-mediated biosynthesis of nanoparticles as an emerging tool against mosquitoes of medical and veterinary importance: a review [J]. Parasitol. Res., 2016,115(1):23-34.
32	XU W, DUBOS C, LEPINIEC L C. Transcriptional control of flavonoid biosynthesis by MYB-bHLH-WDR complexes [J]. Trends Plant Sci., 2015,20(3):176-185.
33	REIMAND J, ARAK T, ADLER P, et al.. g:Profiler--a web server for functional interpretation of gene lists (2011 update) [J]. Nucleic Acids Res., 2011,39:W307-W315.
34	VAN DER HEIJDEN M G A, MARTIN F M, SELOSSE M A, et al. Mycorrhizal ecology and evolution: the past, the present, and the future [J]. New Phytol., 2015,205(4):1406-1423.
35	VERMA V, RAVINDRAN P, KUMAR P P. Plant hormone-mediated regulation of stress responses [J/OL]. BMC Plant Biol., 2016,16(1):86 [2022-02-04]. .
36	BORTESI L, FISCHER R. The CRISPR/Cas9 system for plant genome editing and beyond [J]. Biotechnol. Adv., 2015,2015,33(1): 41-52.
37	刘晓,王慧媛,熊燕,等.基因合成与基因组编辑[J]. 中国细胞生物学学报, 2019, 41(11): 2072-2082.
	LIU X, WANG H Y, XIONG Y, et al.. Progress in gene synthesis and genome editing [J]. Chin. J Cell Biol., 2019, 41(11): 2072-2082.
38	BALTES N J, VOYTAS D F. Enabling plant synthetic biology through genome engineering [J]. Trends Biotechnol., 2015,33(2):120-31.
39	周正,李卿,陈万生,等.药用植物天然产物生物合成途径及关键催化酶的研究策略[J].生物技术通报, 2021, 37(8): 25-34.
	ZHOU Z, LI Q, CHEN W S, et al.. Research strategies of natural products biosynthesis pathways and key enzymes in medicinal plants [J]. Biotech. Bull., 2021, 37(8): 25-34.
40	陈国强,王颖.中国"合成生物学"973 项目研究进展[J].生物工程学报, 2015, 31(6): 995-1008.
	CHEN G Q, WANG Y. Progress in synthetic biology of "973 Funding Program" in China [J]. Chin. J. Biotech., 2015, 31(6): 995-1008.
41	SI T, ZHAO H M. A brief overview of synthetic biology research programs and roadmap studies in the United States [J]. Synth. Syst. Biotech.,2016,1(4):258-264.
42	科学技术部政务服务平台.关于国家重点研发计划"合成生物学"重点专项2018年度项目安排公示的通知[EB/OL]. (2021-07-09) [2022-03-05]. .
43	CHEN G Q. Synthetic biology in China, UK and US [J/OL]. Synth. Syst. Biotech.,2016,1(4):215 [2022-02-04]. .
44	周光明, 陈大明, 熊燕, 等.英国合成生物学规划及其影响与启示[J].中国细胞生物学学报,2019, 41(11): 2091-2100.
	ZHOU G M, CHEN D M, XIONG Y, et al. UK Synthetic biology strategic planning and its enlightenment [J]. Chin. J. Cell Biol., 2019, 41(11): 2091-2100.
45	CLARKE L J, KITNEY R I. Synthetic biology in the UK-An outline of plans and progress [J]. Synth. Syst. Biotech.,2016,1(4):243-257.
46	WILLIAMS G. Australia's Synthetic Biology Roadmap [R/OL]. (2021-09-07) [2022-02-05]. .
47	中华人民共和国农业农村部.农业用基因编辑植物安全评价指南(试行) [EB/OL]. (2022-01-24) [2022-03-05]. .
48	The National Engineering Biology Steering Committee. Engineering Biology: A platform technology to fuel multisector economic recovery and biomanufacturing in Canada [R/OL].(2020-11)[2022-02-05]..

国家 Country	发文量 Published articles	总被引频次 Total citations	篇均被引频次 Citations per article
中国 China	11 874	147 210	12.4
美国 USA	6 501	102 992	15.8
德国 Germany	2 380	38 105	16.0
日本 Japan	2 231	26 797	12.0
印度 India	2 009	33 825	16.8
法国 France	1 908	24 337	12.7
英国 England	1 347	20 216	15.0
西班牙 Spain	1 296	16 907	13.0
加拿大 Canada	1 283	16 078	12.5
韩国 South Korea	1 244	22 524	18.1
澳大利亚 Australia	1 154	16 582	14.3
意大利 Italy	1 141	16 631	14.5
巴西 Brazil	965	8 678	8.9
伊朗 Iran	786	9 122	11.6
荷兰 Netherlands	525	10 157	19.3
波兰 Poland	476	8 529	17.9
瑞士 Switzerland	471	8 406	17.8
比利时 Belgium	452	6 961	15.4
瑞典 Sweden	387	7 578	19.5
丹麦 Denmark	362	7 125	19.6

国家 Country	发文量 Published articles	总被引频次 Total citations	篇均被引频次 Citations per article
中国 China	11 874	147 210	12.4
美国 USA	6 501	102 992	15.8
德国 Germany	2 380	38 105	16.0
日本 Japan	2 231	26 797	12.0
印度 India	2 009	33 825	16.8
法国 France	1 908	24 337	12.7
英国 England	1 347	20 216	15.0
西班牙 Spain	1 296	16 907	13.0
加拿大 Canada	1 283	16 078	12.5
韩国 South Korea	1 244	22 524	18.1
澳大利亚 Australia	1 154	16 582	14.3
意大利 Italy	1 141	16 631	14.5
巴西 Brazil	965	8 678	8.9
伊朗 Iran	786	9 122	11.6
荷兰 Netherlands	525	10 157	19.3
波兰 Poland	476	8 529	17.9
瑞士 Switzerland	471	8 406	17.8
比利时 Belgium	452	6 961	15.4
瑞典 Sweden	387	7 578	19.5
丹麦 Denmark	362	7 125	19.6

机构 Organization	发文量 Published articles	总被引频次 Total citations	篇均被引频次 Citations per article
中国科学院 Chinese Academy of Sciences	1 985	41 679	21.0
中国农业科学院 Chinese Academy of Agricultural Sciences	1 359	17 716	13.0
加州大学系统 University of California System	992	23 464	23.6
美国农业部 United States Department of Agriculture	887	14 678	16.5
南京农业大学 Nanjing Agricultural University	852	10 523	12.3
法国国家科学研究中心 Centre National de la Recherche Scientifique	820	14 800	18.0
法国国家农业食品与环境研究院 INRAE	749	14 240	19.0
西班牙高等学术研究委员会 Consejo Superior de Investigaciones Científicas	663	11 069	16.7
华中农业大学 Huazhong Agricultural University	619	10 793	17.4
马克斯·普朗克学会 Max Planck Society	608	13 925	22.9
西北农林科技大学 Northwest A&F University China	604	8 434	13.9
中国农业大学 China Agricultural University	595	8 559	14.3
浙江大学 Zhejiang University	593	9 598	16.1
美国能源部 United States Department Of Energy Doe	592	14 024	23.6
英国研究与创新局 Uk Research Innovation Ukri	438	11 865	27.0
英国生物技术和生物科学研究委员会 The Biotechnology and Biological Sciences Research Council	425	11 661	27.4
加州大学戴维斯分校 UC Davis	400	7 736	19.3
佛罗里达州立大学系统 Florida State University	398	5 833	14.6
印度科学与工业研究理事会 CSIR	378	5 317	14.0
华南农业大学 South China Agricultural University	365	7 551	20.6

机构 Organization	发文量 Published articles	总被引频次 Total citations	篇均被引频次 Citations per article
中国科学院 Chinese Academy of Sciences	1 985	41 679	21.0
中国农业科学院 Chinese Academy of Agricultural Sciences	1 359	17 716	13.0
加州大学系统 University of California System	992	23 464	23.6
美国农业部 United States Department of Agriculture	887	14 678	16.5
南京农业大学 Nanjing Agricultural University	852	10 523	12.3
法国国家科学研究中心 Centre National de la Recherche Scientifique	820	14 800	18.0
法国国家农业食品与环境研究院 INRAE	749	14 240	19.0
西班牙高等学术研究委员会 Consejo Superior de Investigaciones Científicas	663	11 069	16.7
华中农业大学 Huazhong Agricultural University	619	10 793	17.4
马克斯·普朗克学会 Max Planck Society	608	13 925	22.9
西北农林科技大学 Northwest A&F University China	604	8 434	13.9
中国农业大学 China Agricultural University	595	8 559	14.3
浙江大学 Zhejiang University	593	9 598	16.1
美国能源部 United States Department Of Energy Doe	592	14 024	23.6
英国研究与创新局 Uk Research Innovation Ukri	438	11 865	27.0
英国生物技术和生物科学研究委员会 The Biotechnology and Biological Sciences Research Council	425	11 661	27.4
加州大学戴维斯分校 UC Davis	400	7 736	19.3
佛罗里达州立大学系统 Florida State University	398	5 833	14.6
印度科学与工业研究理事会 CSIR	378	5 317	14.0
华南农业大学 South China Agricultural University	365	7 551	20.6

序号 No.	题名 Title	通信作者 Corresponding author	通信作者所在机构 Organization of corresponding author	出版期刊 Published journal	被引频次 Citations	参考文献 Reference
1	MYB transcription factors in Arabidopsis	DUBOS C	法国国家农业科学研究院 Institut National de Recherche Agronomique	Trends in Plant Science	1 790	［17］
2	Proline： a multifunctional amino acid	SZABADOS L	匈牙利科学院生物研究中心 Institute of Genetics Biological Research Center of the Hungarian Academy of Sciences	Tree Physiology	2 216	［18］
3	Lignin valorization： improving lignin processing in the biorefinery	RAGAUSKAS A J	佐治亚理工学院 Georgia Institute of Technology	Science	2 079	［19］
4	Phenylpropanoid biosynthesis	VOGT T	莱布尼茨植物生物化学研究所Leibniz Institute of Plant Biochemistry	Molecular Plant	1 405	［20］
5	Jasmonates： biosynthesis， perception， signal transduction and action in plant stress response， growth and development. An update to the 2007 review in annals of botany	WASTERNACK C	莱布尼茨植物生物化学研究所Leibniz Institute of Plant Biochemistry	Annals of Botany	1 350	［21］
6	Hormone crosstalk in plant disease and defense： more than just JASMONATE-SALICYLATE antagonism	ROBERT-SEILANIANTZ A	剑桥大学University of Cambridge	Annual Review of Phytopathology	1 104	［22］
7	A TALE nuclease architecture for efficient genome editing	REBAR E J	桑莫生物科技公司（美国） Sangamo BioSciences， Inc. （USA）	Nature Biotechnology	1 461	［23］
8	Genome editing with engineered zinc finger nucleases	GREGORY P D	桑莫生物科技公司（美国） Sangamo BioSciences， Inc. （USA）	Nature		［24］
9	Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome	CHALHOUB B	法国国家农业研究院；埃夫里大学 Institut National de Recherche Agronomique （INRA）；Université d’Evry Val d’Essone， Unité de Recherche en Génomique Végétale	Science	1 271	［25］
10	Green synthesis of metal nanoparticles using plants	IRAVANI S	伊斯法罕大学 Isfahan University	Green Chemistry	1 565	［26］