大豆SWEET 基因在荚粒发育过程中与逆境胁迫下的表达

doi:10.13304/j.nykjdb.2022.1087

摘要/Abstract

摘要：

植物叶片光合作用产生的糖类物质需要经过糖转运蛋白运输到其他器官进而发挥其重要功能。SWEET（sugars will eventually be exported transporters）是一类可实现糖类物质运输的蛋白，对植物生长发育及抵御外界生物和非生物胁迫具有重要意义。为分析大豆SWEET基因在荚粒发育过程中以及逆境胁迫下的表达，利用栽培大豆和野生大豆最新公布基因组数据鉴定SWEET基因，然后利用转录组数据分析基因在荚粒发育和抵抗花叶病毒与低磷逆境中的表达。结果表明，栽培大豆Williams82最新版本基因组（Wm82a4v1）有48个SWEET基因，分布于15条染色体，编码蛋白长度为174~354个氨基酸；野生大豆W05基因组有51个SWEET基因，分布于16条染色体，编码蛋白长度为84~392个氨基酸；系统进化树分析显示，99个栽培大豆和野生大豆SWEET基因分为3个亚组。对不同大豆品种转录组数据分析发现，16个SWEET基因在豆荚表达，其中Glyma.06G122200、Glyma.14G159900和Glyma.14G160100等随不同品种豆荚发育进程表达量增加；12个SWEET基因在籽粒表达，其中Glyma.08G025100、Glyma.13G041300和Glyma.14G120300随不同品种籽粒发育进程表达量增加，表明其在豆荚和籽粒发育中具有重要作用。接种大豆花叶病毒后，不同抗性品种间SWEET基因的表达存在较大差别，其中Glyma.08G009900和Glyma.13G264400在抗病品种叶片接种后诱导表达，而在感病品种接种前后表达量没有变化，说明其可能参与大豆抗病反应。对SWEET基因在低磷胁迫前后的表达分析发现，Glyma.04G198400、Glyma.14G160100和Glyma.15G211800等在大豆根系受低磷胁迫诱导表达，可能参与大豆耐低磷反应。栽培大豆SWEET基因单核苷酸多态性（single nucleotide polymorphism，SNP）等位变异分析发现，有43个SWEET基因含有220个非同义突变SNPs，其中103个位于编码蛋白的保守域，可能影响基因功能。研究结果为大豆豆荚和籽粒产量以及抗病耐逆分子育种提供了基因资源。

关键词: 大豆, SWEET基因, 荚粒发育, 逆境胁迫, 差异表达

Abstract:

Sugars are always transported to the other organs via sugar transporters to play their important functions in plants. SWEET （sugars will eventually be exported transporters） is a kind of sugar transporters which plays important function in plant growth and development as well as in resistance or tolerance to diverse biotic and abiotic stresses. To analyze the expressions of SWEET genes during pod and seed developments and under different stress conditions in soybean， the SWEET genes were firstly analyzed based on the newly published cultivated and wild soybean genomes in the present study. Subsequently， the gene expressions were evaluated during pod and seed developments， as well as in resistance to soybean mosaic virus （SMV） and tolerance to low phosphorus. The results showed that 48 SWEET genes were identified in the cultivated soybean genome （Wm82a4v1）， which encoded protein with length 174~354 amino acids and located on 15 chromosomes. Meanwhile， 51 SWEET genes were identified in the wild soybean genome （W05）， which encoded length of 84~392 amino acids and located on 16 chromosomes. The phylogenetic tree of SWEET genes in cultivated and wild soybeans showed that they were clustered into 3 sub-groups. Moreover， 16 SWEET genes were expressed in soybean pods with the expressions of Glyma.06G122200， Glyma.14G159900 and Glyma.14G160100 etc. increased during the pod development process. 12 SWEET genes were expressed in soybean seeds， and the expressions of Glyma.08G025100， Glyma.13G041300 and Glyma.14G120300 were increased during the seed development， suggesting their important roles for soybean seed development. The SWEET genes showed different expressions after the inoculation of SMV， and the expressions of Glyma.08G009900 and Glyma.13G264400 were induced in leaves of resistant variety， while had no change in the sensitive variety， implying that they might involve in SMV resistance. The expressions of Glyma.04G198400， Glyma.14G160100 and Glyma.15G211800 were induced in soybean roots after low phosphorus treatment， indicating their important functions in the tolerance to low phosphorus stress. In addition， the single nucleotide polymorphism（SNP）analysis of SWEET genes showed that 43 genes contained 220 nonsynonymous SNPs with 103 located in the conserved domains of encoding proteins. Thus， above results provided candidate genes for molecular breeding of soybean pod and seed yield and diverse stress resistances.

Key words: soybean, SWEET gene, pod and seed development, stress, differential expressions

中图分类号:

S565.1

柯博洋, 李文龙, 张彩英. 大豆SWEET 基因在荚粒发育过程中与逆境胁迫下的表达[J]. 中国农业科技导报, 2023, 25(8): 33-52.

Boyang KE, Wenlong LI, Caiying ZHANG. Expressions of SWEET Genes During Pod and Seed Developments and Under Different Stress Conditions in Soybean[J]. Journal of Agricultural Science and Technology, 2023, 25(8): 33-52.

图/表 17

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Soybase基因号^a Gene ID in Soybase	起始位置 Start position	终止位置 End position	CDS长度 CDS length/bp	蛋白长度Protein length/aa	以往研究基因名称^b Gene name in previous study	Soybase基因号^c Gene ID in Soybase
Glyma.12G234500	40 833 964	40 836 337	708	235	GmSWEET29	Glyma12G36300
Glyma.13G002700	811 844	814 553	774	257	GmSWEET32	Glyma13G10560
Glyma.13G037900	10 829 620	10 833 005	747	248	GmSWEET31	Glyma13G09140
Glyma.13G041300	12 351 382	12 354 214	768	255	GmSWEET30	Glyma13G08190
Glyma.13G169700	27 784 626	27 788 932	738	245	GmSWEET33	Glyma13G23860
Glyma.13G264400	36 140 787	36 144 014	708	235	GmSWEET34	Glyma13G33950
Glyma.14G120300	33 992 941	33 996 064	765	254	GmSWEET36	Glyma14G27610
Glyma.14G159900	37 588 876	37 591 423	783	260	GmSWEET37	Glyma14G30740
Glyma.14G160100	37 857 725	37 860 537	765	254	GmSWEET38	Glyma14G30940
Glyma.15G049200	3 861 273	3 863 135	750	249	GmSWEET39	Glyma15G05470
Glyma.15G149800	12 363 010	12 366 891	738	245	GmSWEET40	Glyma15G16030
Glyma.15G210400	33 183 156	33 190 881	672	223	GmSWEET41	Glyma15G27530
Glyma.15G211800	33 761 630	33 764 272	708	235	GmSWEET42	Glyma15G27750
Glyma.17G090800	7 074 090	7 076 820	681	226	GmSWEET43	Glyma17G09840
Glyma.18G295100	57 533 652	57 535 707	789	262	GmSWEET44	Glyma18G53250
Glyma.18G301100	58 162 823	58 164 693	807	268	GmSWEET45	Glyma18G53930
Glyma.18G301200	58 172 252	58 175 319	816	271	GmSWEET46	Glyma18G53940
Glyma.19G009800	912 713	917 261	741	246	GmSWEET47	Glyma19G01270
Glyma.19G009900	918 263	925 358	741	246	GmSWEET48	Glyma19G01280
Glyma.19G232200	48 770 032	48 773 696	924	307	GmSWEET49	Glyma19G42040
Glyma.20G066500	23 504 782	23 507 682	771	256	GmSWEET51	Glyma20G16160
Glyma.20G082700	31 085 642	31 087 512	543	180	—	—

Soybase基因号^a Gene ID in Soybase	起始位置 Start position	终止位置 End position	CDS长度 CDS length/bp	蛋白长度Protein length/aa	以往研究基因名称^b Gene name in previous study	Soybase基因号^c Gene ID in Soybase
Glyma.12G234500	40 833 964	40 836 337	708	235	GmSWEET29	Glyma12G36300
Glyma.13G002700	811 844	814 553	774	257	GmSWEET32	Glyma13G10560
Glyma.13G037900	10 829 620	10 833 005	747	248	GmSWEET31	Glyma13G09140
Glyma.13G041300	12 351 382	12 354 214	768	255	GmSWEET30	Glyma13G08190
Glyma.13G169700	27 784 626	27 788 932	738	245	GmSWEET33	Glyma13G23860
Glyma.13G264400	36 140 787	36 144 014	708	235	GmSWEET34	Glyma13G33950
Glyma.14G120300	33 992 941	33 996 064	765	254	GmSWEET36	Glyma14G27610
Glyma.14G159900	37 588 876	37 591 423	783	260	GmSWEET37	Glyma14G30740
Glyma.14G160100	37 857 725	37 860 537	765	254	GmSWEET38	Glyma14G30940
Glyma.15G049200	3 861 273	3 863 135	750	249	GmSWEET39	Glyma15G05470
Glyma.15G149800	12 363 010	12 366 891	738	245	GmSWEET40	Glyma15G16030
Glyma.15G210400	33 183 156	33 190 881	672	223	GmSWEET41	Glyma15G27530
Glyma.15G211800	33 761 630	33 764 272	708	235	GmSWEET42	Glyma15G27750
Glyma.17G090800	7 074 090	7 076 820	681	226	GmSWEET43	Glyma17G09840
Glyma.18G295100	57 533 652	57 535 707	789	262	GmSWEET44	Glyma18G53250
Glyma.18G301100	58 162 823	58 164 693	807	268	GmSWEET45	Glyma18G53930
Glyma.18G301200	58 172 252	58 175 319	816	271	GmSWEET46	Glyma18G53940
Glyma.19G009800	912 713	917 261	741	246	GmSWEET47	Glyma19G01270
Glyma.19G009900	918 263	925 358	741	246	GmSWEET48	Glyma19G01280
Glyma.19G232200	48 770 032	48 773 696	924	307	GmSWEET49	Glyma19G42040
Glyma.20G066500	23 504 782	23 507 682	771	256	GmSWEET51	Glyma20G16160
Glyma.20G082700	31 085 642	31 087 512	543	180	—	—

Soybase 基因号^a Gene ID in Soybase	起始位置 Start position	终止位置 End position	CDS长度 CDS length/bp	蛋白长度Protein length/aa	以往研究基因名称^b Gene name in previous study	Soybase基因号^c Gene ID in Soybase
Glyma.02G088400	7 718 092	7 719 984	789	262	GmSWEET1	Glyma02G09710
Glyma.03G065500	11 974 987	11 979 388	711	236	—	—
Glyma.03G209600	42 804 561	42 807 560	1 065	354	GmSWEET2	Glyma03G36790
Glyma.04G198400	45 886 443	45 889 300	777	258	GmSWEET4	Glyma04G37510
Glyma.04G198500	45 896 868	45 899 911	849	282	GmSWEET5	Glyma04G37520
Glyma.04G198600	45 908 432	45 910 736	951	316	GmSWEET6	Glyma04G37530
Glyma.04G238100	49 480 357	49 482 035	525	174	GmSWEET7	Glyma04G41680
Glyma.04G241400	49 764 845	49 768 113	744	247	GmSWEET8	Glyma04G42040
Glyma.05G036500	3 168 199	3 171 964	681	226	GmSWEET9	Glyma05G02070
Glyma.05G122200	31 617 834	31 620 408	852	283	GmSWEET10	Glyma05G25180
Glyma.05G202600	38 618 752	38 621 477	711	236	—	—
Glyma.05G202700	38 630 962	38 633 524	774	257	GmSWEET11	Glyma05G38340
Glyma.06G122200	9 927 550	9 932 286	777	258	GmSWEET13	Glyma06G12740
Glyma.06G125800	10 236 053	10 237 949	765	254	GmSWEET14	Glyma06G13110
Glyma.06G166800	13 866 485	13 868 782	930	309	GmSWEET15	Glyma06G17520
Glyma.06G166900	13 885 783	13 888 665	783	260	GmSWEET16	Glyma06G17530
Glyma.06G167000	13 899 833	13 902 581	777	258	GmSWEET17	Glyma06G17540
Glyma.06G200800	18 221 597	18 227 992	555	184	—	—
Glyma.08G009900	774 563	777 046	885	294	GmSWEET20	Glyma08G01300
Glyma.08G010000	784 374	787 011	765	254	GmSWEET21	Glyma08G01310
Glyma.08G025100	1 979 341	1 982 928	744	247	GmSWEET22	Glyma08G02890
Glyma.08G077200	5 873 073	5 875 662	825	274	GmSWEET23	Glyma08G08200
Glyma.08G183500	14 806 104	14 808 441	843	280	GmSWEET24	Glyma08G19580
Glyma.08G360400	46 604 757	46 607 236	819	272	GmSWEET25	Glyma08G47550
Glyma.08G360500	46 611 971	46 613 923	822	273	—	—
Glyma.09G043200	3 665 263	3 673 798	735	244	GmSWEET28	Glyma09G04840

Soybase 基因号^a Gene ID in Soybase	起始位置 Start position	终止位置 End position	CDS长度 CDS length/bp	蛋白长度Protein length/aa	以往研究基因名称^b Gene name in previous study	Soybase基因号^c Gene ID in Soybase
Glyma.02G088400	7 718 092	7 719 984	789	262	GmSWEET1	Glyma02G09710
Glyma.03G065500	11 974 987	11 979 388	711	236	—	—
Glyma.03G209600	42 804 561	42 807 560	1 065	354	GmSWEET2	Glyma03G36790
Glyma.04G198400	45 886 443	45 889 300	777	258	GmSWEET4	Glyma04G37510
Glyma.04G198500	45 896 868	45 899 911	849	282	GmSWEET5	Glyma04G37520
Glyma.04G198600	45 908 432	45 910 736	951	316	GmSWEET6	Glyma04G37530
Glyma.04G238100	49 480 357	49 482 035	525	174	GmSWEET7	Glyma04G41680
Glyma.04G241400	49 764 845	49 768 113	744	247	GmSWEET8	Glyma04G42040
Glyma.05G036500	3 168 199	3 171 964	681	226	GmSWEET9	Glyma05G02070
Glyma.05G122200	31 617 834	31 620 408	852	283	GmSWEET10	Glyma05G25180
Glyma.05G202600	38 618 752	38 621 477	711	236	—	—
Glyma.05G202700	38 630 962	38 633 524	774	257	GmSWEET11	Glyma05G38340
Glyma.06G122200	9 927 550	9 932 286	777	258	GmSWEET13	Glyma06G12740
Glyma.06G125800	10 236 053	10 237 949	765	254	GmSWEET14	Glyma06G13110
Glyma.06G166800	13 866 485	13 868 782	930	309	GmSWEET15	Glyma06G17520
Glyma.06G166900	13 885 783	13 888 665	783	260	GmSWEET16	Glyma06G17530
Glyma.06G167000	13 899 833	13 902 581	777	258	GmSWEET17	Glyma06G17540
Glyma.06G200800	18 221 597	18 227 992	555	184	—	—
Glyma.08G009900	774 563	777 046	885	294	GmSWEET20	Glyma08G01300
Glyma.08G010000	784 374	787 011	765	254	GmSWEET21	Glyma08G01310
Glyma.08G025100	1 979 341	1 982 928	744	247	GmSWEET22	Glyma08G02890
Glyma.08G077200	5 873 073	5 875 662	825	274	GmSWEET23	Glyma08G08200
Glyma.08G183500	14 806 104	14 808 441	843	280	GmSWEET24	Glyma08G19580
Glyma.08G360400	46 604 757	46 607 236	819	272	GmSWEET25	Glyma08G47550
Glyma.08G360500	46 611 971	46 613 923	822	273	—	—
Glyma.09G043200	3 665 263	3 673 798	735	244	GmSWEET28	Glyma09G04840

Soybase基因号 Gene ID in Soybase	起始位置 Start position	终止位置 End position	编码区长度 Encoding region length/bp	蛋白长度 Protein length/aa
Glyso.W05.03g007786	43 483 758	43 485 728	1 065	354
Glyso.W05.04g010276	46 663 345	46 666 196	777	258
Glyso.W05.04g010277	46 673 703	46 676 709	849	282
Glyso.W05.04g010278	46 685 202	46 687 351	951	316
Glyso.W05.04g010648	50 276 230	50 277 418	525	174
Glyso.W05.04g010679	50 556 688	50 565 022	744	247
Glyso.W05.05g011193	3 163 834	3 167 295	702	233
Glyso.W05.05g012088	33 484 789	33 487 327	852	283
Glyso.W05.05g012863	40 515 232	40 517 709	888	295
Glyso.W05.05g012864	40 527 179	40 529 765	774	257
Glyso.W05.06g014484	10 042 877	10 047 769	744	247
Glyso.W05.06g014520	10 349 792	10 351 809	765	254
Glyso.W05.06g014913	13 980 864	13 983 203	930	309
Glyso.W05.06g014914	14 000 327	14 003 213	783	260
Glyso.W05.06g014915	14 014 412	14 017 191	777	258
Glyso.W05.08g019272	786 619	789 128	885	294
Glyso.W05.08g019273	796 573	799 075	765	254
Glyso.W05.08g019421	1 989 104	1 992 705	744	247
Glyso.W05.08g019912	5 908 560	5 911 103	825	274
Glyso.W05.08g020934	14 817 339	14 819 722	843	280
Glyso.W05.08g022680	47 501 706	47 504 145	819	272
Glyso.W05.08g022681	47 508 969	47 510 852	822	273
Glyso.W05.09g023167	3 632 699	3 641 090	735	244
Glyso.W05.09g023919	26 973 647	26 974 159	417	138
Glyso.W05.11g030581	24 808 431	24 808 848	255	84
Glyso.W05.11g030961	36 013 282	36 017 219	738	245
Glyso.W05.11g031878	44 349 437	44 353 044	708	235
Glyso.W05.12g035305	43 852 265	43 855 848	708	235
Glyso.W05.13g035416	773 032	775 298	774	257
Glyso.W05.13g035646	11 739 631	11 743 028	747	248
Glyso.W05.13g035684	13 262 074	13 265 093	768	255
Glyso.W05.14g038816	34 653 572	34 656 886	750	249
Glyso.W05.14g038879	38 662 759	38 664 694	858	285
Glyso.W05.14g038881	38 928 839	38 931 799	765	254
Glyso.W05.15g040012	3 865 965	3 867 423	807	268